ABSTRACT: The present investigation was undertaken to evaluate potential inhibitory effect of indigenous bio-control agents against Fusarium oxysporum f. sp. Lycopersici, (FOL), a causal organism of wilt disease in tomato crops. Twenty indigenous Trichoderma species were isolated from tomato rhizosphere soil collected from tomato growing fields in and around Mysore district, which is geographically located in Southern part of Karnataka, India. Amongst twenty Trichoderma isolates, eight isolates displayed significant activity against the test pathogen. Among the eight isolates two isolates, T. harzianum and T. viride, exhibited excellent inhibitory effects on the test pathogen in dual culture technique. The obtained preliminary results are valuable and promising enough for further studies towards isolation and characterization of antifungal agent responsible for activity.
Key words: Tomato wilt, Fusarium oxysporum, Trichoderma, Rhizospheric soil, Bio control agents.
[1] H. Behzad, T. G. Mousa, R. M. Mohammad, and D. Mahdi, Biological potential of some Iranian Trichoderma isolates in the control of soil borne plant pathogenic fungi, African Journal of Biotechnology, 8, 2008, 967-972.
[2] D. K. Bell, H. D. Wells, and C. R. Markham, In vitro antagonism of Trichoderma species against six fungal plant pathogens, Phytopathology, (72), 1982, 379-382.
[3] L.W Burgess, B. A. Summerell, S Bullock, K. P. Gott, and D. Backhouse, Laboratory manual for Fusarium research, 3, 1994, 9-11.
[4] J. P. Gilreath, J. P. Jones, A. J. Overman, Soil borne pest control in mulched tomato with alternative to methyl bromide. Proceedings of the Florida State Horticultural Society, 107, 1994, 156-159.
[5] ISTA, International rules for seed testing, Rules Draper (ed) Switzerland, The International Seed Testing Association, 2003, 1- 47.
[6] R. Kumar Kiran, K. S. Jagadeesh, P. U. Krishnaraj, M. S. Patil, Enhanced growth promotion of tomato and nutrient uptake by plant growth promoting rhizobacterial isolates in presence of tobacco mosaic virus pathogen, Karnataka Journal of Agricultural Sciences, 2, 2008, 309-311.
[7] M. Marlatt, G. Gilardi, M. L. Gullino, A. Garibaldi, Biological control potential of Achromobacterxylosoxydoms for suppressing Fusarium wilt of tomato, International Journal of Botany, 4, 2008, 369-375.
[8] M. Radwan, Barakat, A. M. Fadel, S. Mohammed, AliShtayeh, I. A. M. Mohammad, Biological Control of Rhizoctonia solani by Indigenous Trichoderma spp. Isolates from Palestine, Hebrew University of Jerusalem, 3, 2007, 1 – 15.
[9] Shafiquzzaman S , Umi K Y , Kausar H, Sarwar J, In vitro studies on the potential Trichoderma harzianum for antagonistic properties against Ganoderma boninense, Journal of Food Agriculture and Environment, 4, 2009, 970 - 976.
[10] V. Shanmugam, N. Kanoujia, Biological management of vascular wilt of tomato caused by Fusarium oxysporum f. sp. lycospersici by plant growth-promoting rhizo-bacterial mixture, Biological control, 57, 2011, 85-93.