Version-1 (Sep–Oct 2015)
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Detection of Alpha-Amylase Activity from Soil Bacteria |
| Country | : | Nigeria |
| Authors | : | Salihu, Y. || Saidu, A. Y. || Rabiu, G. A. || Umar A.A. || Zeynep, A. || Unzile, G. |
Abstract: Background: Alpha-amylase is one of the industrial enzymes that hydrolyze starch molecules into polymers composed of glucose units. The enzyme has potential application in a wide number of industrial processes such as food, textile, paper, detergent, fermentation and pharmaceutical industries. Alpha-amylase can be produced by microorganisms, plants or animals. Aim: The aim of this study is to detect the activity of alpha-amylase from bacteria isolated from soil environment.
[1] Pandey, A., Nigam P., Soccol VT., Singh D. and Mohan R. Advances in microbial amylases. Biotechnol. Appl. Biochem, Vol. 31, pp. 135-152, 2000.
[2] Kathiresan, K. and Manivannan S. Alpha-amylase production by Penicillium fellutanum isolated from mangrove rhizospheric soil. Afr. J. Biotechnol, Vol. 5, pp. 829-832, 2006.
[3] Schmidt, S., Martin A., Schadt, C., Lipson, D., Meyer, A., Costello, E., Nemergut, D., West, A., and Metchalf, J. Profound seasonal changes in microbial diversity and function Alpine Environment. Mc-Graw Hill, New York, pp. 776-795, 1991.
[4] Rusell, E.W. Soil conditions and plant Growth. 10th eds. William Clowes and Sons Ltd., London, pp. 150, 1977.
[5] Octavio, L.F., Daniel, J.R., Francislete, R.M., Carlos Bloch Jr., Carlos, P.S. and Maria F.G. Activity of wheat alpha-amylase inhibitors towards burchid alpha-amylase and structural explanations of observed specificities. Eur. J. Biochem, Vol. 267, pp. 2166-2173, 2000.
[6] Richele, D.W., M.B. and Lubbert, D. Engineering of factors determining alpha-amylase and cyclodextrin glycosyltransferase specificity in the cyclodextrin glycosyltransferase from Themoanaerobacterium thermosulfurigenes EMI. European Journal of Biochemistry, Vol. 253 (3), pp. 598-605, 1998.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | DNA construct instability in bacteria used for Agrobacterium mediated plant transformation |
| Country | : | Nigeria |
| Authors | : | Ihuoma C. Okwuonu || Ome K. Achi || Uchechi N. Ekwenye || Solomon O. Afuape || Francis A.Nkaa || Chiedozie N Egesi. |
Abstract: The use of plasmid in the production of genetically modified (GM) crops is highly essential in research and in commercial production of GM plants. However plasmid instability constitutes a major problem in the use of recombined microorganisms in the production of GM crops. In this study we evaluated the stability of p8114 carrying a gene coding for a transcription factor (TFIIIA) driven by Cassava Vein Mosaic Virus (CsVMV) promoter and an nptII selectable marker driven by 35S promoter in the T-DNA. The plasmid was amplified in E.coliDH5α strain on Luria Broth (LB)agar supplemented with 100 μg/ml kanamycin. The colonies were confirmed by Restriction Fragment Length Analysis (RFLA) and by DNA sequencing. The confirmed colonies were stored as glycerol stock at -800C and as DNA extracts in TE buffer at 40C. Agrobacterium strains LBA4404, EHA 105 and AGL1 were also transformed with DNA from the confirmed colonies. Plasmid stability was evaluated after 3 months.
[1]. N. Kuniechick, R. Munareto, T. Milech de Assunção, G. Volpato, J. Chies, C. PaivaNunes, and D. Santiago Santos. "Determination of the stability of recombinant plasmid containing the coding sequence of the human growth hormone in bioreactor."XSalão de IniciaçãoCientífica PUCRS, 2009, 2829-2831.
[2]. A. Guruatma, K. Jiaquot;. Mama Ji's Molecular Kitchen. ASU - Ask A Biologist. Retrieved September 10, 2015 from http://askabiologist.asu.edu/plasmids
[3]. P. Kumar, H. Maschke, K. Friehs, and K. Schügerl. Strategies for improving plasmid stability in genetically modified bacteria in bioreactors. Trends in biotechnology, 9(1), 1991, 279-284.
[4]. F. Hoffmann, and U. Rinas. Stress induced by recombinant protein production in Escherichia coli. In Physiological Stress Responses in Bioprocesses Springer Berlin Heidelberg 2004, 73-92.
[5]. M. Fakruddin, R. Mohammad Mazumdar, K. S. Bin Mannan, A. Chowdhury and M. N. Hossain, Critical factors affecting the success of cloning, expression, and mass production of enzymes by recombinant E. coli. ISRN biotechnology, 2013.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Polyesters From Chemically Modified Oil of Renewable Source |
| Country | : | Nigeria |
| Authors | : | O.FOsagie || Oriji A.B |
Abstract: Rubber seeds, agricultural wastes without any known nutritional value, were blended to obtain oil. The oil was employed in the production of polyesters (alkyd resins)usingalcoholysis method. The rubber seed oil (RSO) and its alkyd resins were characterized and evaluated by standard methods for their physicochemical properties, drying schedule and chemical endurance. RSO had an iodine value of 99.40, saponification values of 202.36, acid value of 22.16, viscosity of 25.72 free fatty acid of 11.20 and golden yellow colour. Also, the RSO prepared polyesters have coating properties whose values are comparable to those of commercial polyesters. It is therefore recommended that the oil obtained from the seeds of natural rubber (agricultural waste) be exploited in Nigeria for the production of environmentally friendly polyesters in other to optimize its application in non pigmented surface coating.
Keywords: Wastes, Rubber seeds, Environmentally friendly, Renewable source, polyesters
[1]. Abdullah B. M.; Salimon O (2009) Physicochemical characteristics of Malaysian rubber (Heverebrasiliensis) seed oil. Eur. J. Sci. Rej., 31, 431-445.
[2]. Abulude, F. O., Ogunkoya, M. O., &Ogunleye, R. F. (2007). Storage properties of oils of two Nigeria oil seeds Jatrophacurcas(physic nut) and Helianthus annuus(sunflower). Amer. J. Food Tech., 2, 207-211. http://dx.doi.org/10.3923/ajft.2007.207.211
[3]. Adefarati, F.B., (1986): Development of Non-Edible Oils for use in the Nigerian Surface Coating Industry with particular reference to Rubber (Heveabrasiliensis) Seed Oil for manufacture of Air-Drying Oil modified Alkyds. In: Enabor EE (ed.) Industrial Utilization of Natural Rubber (Heveabrasiliensis) seed, latex and wood, Rubber Research Institue of Nigeria. 27-38.
[4]. Aigbodion, A.I (1991): Prelimanary Studies on the preparation of rubber seed oil, Indian journal of Natural Rubber Resaerch, 4(2): 114-117.
[5]. Aigbodion, A.I and Pillai, C.K.S (2001); Synthesis and molecular weight characterization of Rubber Seed Oil- modified Alkyd Resin; Journal of Applied Polymer Science. Vol. 32, No9, pp 1105-1108.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Cystatin C as a marker of Cardio metabolic disorder in obese South Indian individuals |
| Country | : | India |
| Authors | : | Deepa K. || Rahul M. H || Shubha Jayaram || Meera S || Sudhir |
Abstract: Background: Human obesity is strongly associated with cardio metabolic disease. Cystatin C is a naturally occurring protease inhibitor and marker of cardiovascular disease. The main objective of present study was to estimate the serum levels of Cystatin C in individuals with normal BMI, and obese, aged between 18-39 Yrs and to compare the levels of serum Cystatin C among these individuals and to correlate the levels of serum Cystatin C with cardio metabolic risk factors. Material & Methods: The study population was taken from healthy volunteers of Mysore city, aged between 18-39 years of either sex. The study population was divided into 2 groups based on BMI. Each group contains sample size of 45. Fasting serum sample was analyzed for Blood glucose, Total cholesterol, Total Triglycerides, Direct HDL cholesterol, Direct LDL Cholesterol by enzymatic method and serum Cystatin-C by immunoturbidimetric method using auto analyser.
[1]. World Health Organization, 'Preventing chronic disease: a vital investment,' WHO global report, World Health Organization, Geneva, Switzerland, 2005.
[2]. Dandona P,Aljada A, Chaudhuri A, Mohanty P, Metabolic syndrome: a comprehensive perspective based on interactions between obesity, diabetes& inflammation. Circulation 2005; 111:1448-1454.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Assessment of genetic fidelity of in vitro propagated clones of Celastrus paniculatus Willd by using RAPD based PCR Amplification |
| Country | : | India |
| Authors | : | Anusha T S || Manju Mohan || Deepti Madayi || Deepthi V C || M V Joseph || K K ELYAS |
Abstract: Celastrus paniculatus Willd belonging to the family Celastaceae is an endangered Indian medicinal plant having high pharmaceutical application. The objective of the present investigation was to assess the the clonal fidelity of in vitro propagated clones of Celastrus paniculatus with the field grown mother plant to confirm their true to type nature. Micropropagation is an alternative method for the large scale production of endangered medicinal plants. The genetic stability of in vitro raised clones of celastrus paniculatus were assessed by using RAPD analysis. Genomic DNA was isolated from healthy and fresh leaves of both mother plant and in vitro raised plants of Celastrus paniculatus by using CTAB method. Based on the reproducibility of the primers, 15 RAPD primers were selected for the present investigation. The selected primers gave rise to a total of 75 scorable bands with an average of 5.1 bands ranging from 300-2700 bp. The number of bands varied from three (OPQ-07, OPA-13) to seven (OPC-20, OPN-16).
[1]. Ahmad N, Anis M An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L. European Journal of Forest Reaearch 2011;130:135-144
[2]. Asthana P, Jaiswal VS, Jaiswal U Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis. Acta Physiologiae 2011;33:1821-1829
[3]. Doyle JJ, Doyle JJ Isolation of plant DNA from fresh tissue. Focus 1990; 12:13-15
[4]. Lal D, Singh N Mass multiplication of Celastrus paniculatus Willd an important medicinal plant under in vitro conditions using nodal segments. Journal of American Science 2010;6:55-61
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Isolation and characterization of lipolytic Pseudomonas spp. from oil contaminated water samples |
| Country | : | India |
| Authors | : | Vivek S. Vishwe || Shrikant P. Gajbhiye || Shashikant P. Vaidya || Abhay S. Chowdhary |
Abstract: Oil contaminated water samples collected from different areas in Pune were screened for the selective isolation of lipolytic Pseudomonas spp. Screening medium containing Tributyrin (1% v/v) and Rhodamine B was used for the specific isolation of lipolytic Pseudomonas spp. from oil contaminated water samples. Lipolytic bacteria showing zone of clearance around the colonies were selected for the present study. Isolated bacteria were identified upto genus level with the help of morphological and biochemical testing by following Bergey's manual. Spectrophotometric Lipase assay showed that out of 49 lipolytic Pseudomonas spp., WP23 exhibits the maximum Lipase activity of about 45.4733 U/ml.
[1]. R. Sharma, Y. Chisti and U.C. Banerjee, Production, purification, characterization, and applications of Lipases: Review, Biotechnology Advances, 19, 2001, 627-662.
[2]. D. Sharma, B. Sharma and A.K. Shukla, Biotechnological Approach of Microbial Lipase: Review, Biotechnology, 10(1), 2011, 23-40.
[3]. N. Verma, S. Thakur and A.K. Bhatt, Microbial Lipases: Industrial Applications and Properties: Review, International Research Journal of Biological Sciences, 1(8), 2012, 88-92.
[4]. R Aravindan, P. Anbumathi and T. Viruthagiri, Lipase applications in food industry: Review, Indian Journal of Biotechnology, 6, 2007, 141-158.
[5]. S. Thakur, Lipases: Its Sources, Properties and Applications: Review, International Journal of Scientific & Engineering Research, 3(7), 2012, 01-29.
[6]. F. Hasan, A.A. Shah, and A. Hameed, Industrial applications of microbial Lipases: Review, Enzyme and Microbial Technology, 39, 2006, 235-251.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Effect Of Exposure To Lead And Cadmium In Drinking Water Sources From Nigeria's Niger Delta Region On Fecundity In Albino Wister Rats. |
| Country | : | Nigeria |
| Authors | : | Eteng, M.U || Berena, G.A || Anacletus, F.C || Bassey, S.C.O |
Abstract: As Nigeria strives towards attaining the Millennium Development Goal for water, this study was carried out to investigate the effect of exposure to lead (Pd) and cadmium (Cd) in drinking water source in the Niger Delta region on fecundity and reproductive organ weights. Prior to the study, levels of heavy metals in samples were assessed and compared to WHO permissible limit. The levels of Pb and Cd were higher than that of WHO limit in some samples selected and used for the study. WHO permissible limit of lead and cadmium in the drinking water are 0.01 and 0 003mg/L respectively. Forty eight albino Wistar rats (210-240g) of both sexes were assigned into three groups: control (16), Pb-treated (16) and Cd-treated (16). The control group was given distilled water, while the Pd-treated and Cd-treated group were administered drinking water randomly collected from natural source with Pd (0.00-0.142mg/L) and Cd (0.00-0.024mg/L/) .
[1]. Alexander, H. Checkoway, H. and Can-Netten, C. (1996). serum quality of men employed at a lead smelter. Occupational and Environmental Medicine, 53:411-416
[2]. Aoyagi, T., Ishikawa, H., Miyaji, k., Hayakawa, K, and Hata, M . (2006). Cadmium-induced testicular damage in a rat model of subchronic intoxication. Reproductive Medicine and Biology, 1:59-63
[3]. Apostoli, P. and Catalani, S. (2011). Metal ions affecting reproduction and development. Metal Ions in life Science, 8:263-303
[4]. Benoff, S., Jacob, A. and Hurley, I.R. (2001). Male infertility and environmental exposure to lead and cadmium. Human Reproductive Update, 6:107-121
[5]. Eteng, M.U. Onwuka, F.C. Umoh, I.B. and Abolaji, A.O. (2008). Transgenerational effects of cadmium toxicity on gonadal steroid levels and reproductive outcomes of Wistar rats, Journal od Applied Sciences Reasearvh, 4(7(:925-928
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Gamma Glutamyl Transferase Activity (Ggt) In Albino Rats Treated With Orphenadol Analgesics |
| Country | : | Nigeria |
| Authors | : | Agbafor K.N. || Ebenyi L.N. || Ominyi M.C. |
Abstract: The use of orphenadol as a wide spectrum painkiller (analgesics) has been reported to elicit some toxic effects. This research examined the hepatobiliary effect of orphenadol solution on hepatobiliary system in albino rats. Twenty male albino rats, distributed into four groups (A, B, C and D), with five rats in each group, were used in this research. Groups A, B and C were given oral treatment of 21, 42 and 84mg/kg respectively of the drug solution for seven days consecutively, while the animals in group D were kept as control. Treatment of animals with the drug sample resulted to a decrease in physical activities, body weights, and feed and water intake during the period of treatment relative to the control. Measurement of the total protein concentration in the serum of the rats did not reveal any significant difference (P>0.05) between the test and the control groups.
[1]. Harper, D. (2012). Analgesic effect of orphenadol. International journal, 14(9): 636 – 7.
[2]. Dworkin, R.H. (2003). Advances in neuropathic pain: diagnosis, mechanisms, and treatment recommendations". Arch. Neurol. 60 (11): 1524–34.
[3]. Backonja, A.A., and Rowbotham, C.E. (2011). Neuropathy and pain associated with it. Pharmacological therapy, 107(5): 1 – 40
[4]. Labout, JJ; Thijssen, C; Keijser, GG; Hespe, W (2002). Difference between single and multiple dose pharmacokinetics of orphenadrine hydrochloride in man. European journal of clinical pharmacology, 21 (4): 343–50.
[5]. ROBITSCHER, J.B., PULVER, S.E. ( 2008). Orphenadrine in the treatment of depression; a preliminary study. The American Journal of Psychiatry, 114 (12): 1113–5.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Glutathione Peroxidase Activity in Albino Rats Treated With Starcimol Extra (Analgesics) |
| Country | : | Nigeria |
| Authors | : | Agbafor K. N. || Onuoha S. C. || Arinya O. F. |
Abstract: The use of analgesic in the treatment/management of pains has been shown to produce several side effects. This research examined the effect of starcimol Extra on the glutathione peroxidase activity in albino rats. Twenty albino rats used in this research were separated and grouped into four (A, B, C and D), five rats per group. Groups A, B and C were treated orally with 22.5, 45.0 and 90.0mg/kg body weight of Starcimol Extra solution for seven (7) successive days, while group D served as the control. There was a decrease in body weight of the treated animals unlike the control which increased in body weight. There was increase in feed and water intake of the treated rats relative to the control.
[1]. Ahmad, L. S. (2010). Management of Aging, Academic Press, Lagos, Nigeria, 173-500.
[2]. Akil, A. and Simon, P. (2003). Mechanism of Opioids, 7th edition, American Press, New York, USA. 7-43.
[3]. Allen, D. D. and Rossel, P. O. (2007). Trends in Oxidative Aging, 7th edition, Oxford University Press, London. 65-87.
[4]. Auret, M. O. (2008). Hepatology and Transplant, 5th edition, Oxford University Press. London, England. 1-23.
[5]. Broe, W. A. (2014). Effects of Paracetamol on Glutathion Peroxidase, American Journal of Glutathione Peroxidase, 5(2): 30-54.
[6]. Clementi, R. O. (2000). Management of Pain, Oxford University Press. London, England. 800-1230.
[7]. Dalton, F. O. (2000). Classification of Pain, 9th edition, American Press, New York, USA. 4500-5432.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Interleukin16 and Interleukin 28B Genes Polymorphism in HBV Infected Saudi patients. |
| Country | : | Egypt |
| Authors | : | Eed EM || HusseinYM || Ghonaim MM || Khalifa AS || Alzahrani SS || Alhazmi AS || Alwakeel HR |
Abstract: The course of hepatitis B virus (HBV) infection is variable depending on many factors. In this study, we investigated single nucleotide polymorphisms of interleukin-28B and interleukin-16 as possible host factors which may determine the occurrence of hepatocellular carcinoma (HCC) in Saudi patients. Chronic hepatitis B (CHB) patients (75), HCC patients (42), and healthy controls (70) were analyzed for polymorphisms of the IL16 and IL28B genes using PCR and restriction fragment length polymorphism (RFLP). Results showed that HCC and chronic HBV patients had higher prevalence of rs11556218TG genotype than controls.
[1] Cheng L, Chen HL, Hsiang H, The I H, Chien W S, Ming CH, Hui CH, Kuei CL, Che CC, Ming W L, Han CL and Shou DL. IL28B polymorphism correlates with active hepatitis in patients with HBeAg-Negative chronic hepatitis B. PLoS ONE 8(2), 2013, e58071. doi:10.1371.
[2] Ganem D and Prince AM. Hepatitis B virus infection – natural history and clinical consequences. N. Engl. J. Med. 2004, 350:1118–1129.
[3] Abdo AA, Sanai MF and Faleh ZA. Epidemiology of viral hepatitis in Saudi Arabia: Are we off the hook? Saudi J. of Gastroenterol. 18 (6),2012, 349-357.
[4] Al-Thaqafy MS, Balkhy HH, Memish Z, Makhdom YM, Ibrahim A, Al-Amri A and Al-Thaqafi A. Hepatitis B virus among Saudi National Guard personnel: seroprevalence and risk of exposure. J. Infect. Public Health 6(4), 2013, 237-245.
[5] Al-Ajlan A (2011) Prevalence of hepatitis B and C among students of health colleges in Saudi Arabia. East Mediterr. Health J.;17(10):759-762.
[6] Ministry of Health of Saudi Arabia (MOH). A review of health situation. The Annual Health Statistics Book 10, 2013, 117-134.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Study of serum magnesium and fasting blood glucose in hypertension |
| Country | : | India |
| Authors | : | Dr.Gulab kanwar || Dr.Surekha kirad || Dr.Lokesh chawala || Dr.Mamta Yadav || Dr.Kusum bala jain |
Abstract: A study was carried out to determine the fasting blood glucose and serum magnesium levels in patients with hypertension. A total of 80 hypertensive patients were evaluated, of which, 58 were males and 22 were females. The mean ages of male and female hypertensive subjects were 48.06±6.53 and 50.83±7.62 respectively. Hypomagnesemia was observed in 61.25% subjects and raised fasting blood glucose was identified in 55.1% of individuals. Therefore occurrence of low serum magnesium and raised blood sugar level in patients with hypertension was observed.
Keywords: Hypertension, Serum Magnesium, fasting blood glucose.
[1]. Giles TD, Berk BC, Black HR, Cohn JN, Kostis JB, Izzo JL, Weber MA, 2005. Expanding the Definition and Classification of Hypertension. Journal of Clinical Hypertension, 7(9): 505-512.
[2]. Datla, S.R. and K.K. Griendling, 2010. Hypertension Highlights Reactive Oxygen Species, NADPH
[3]. Oxidases and Hypertension. Hypertension, 56: 325-330.
[4]. Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J, 2005. Global burden of hyper tension: Analysis of worldwide data. Lancet, 365(9455): 217-223.
[5]. Chobanian AV, Bakris GL, Black HR, Cushman WC, Green LA, Izzo, JL, Jones DW, Materson BJ, Oparil S, Wright JT, Roccella EJ, 2003. Seventh report of the joint national committee on prevention, detection, Evaluation and treatment of high blood pressure. Hypertension, 42: 1206-52.
[6]. Gindler EM, Heth DA, 1971. Colorimetric determination with bound calmagite of magnesium in human blood serum. Clinical Chemistry, 17: 662.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Expression Purification and Immunodetection of a fusion protein Glutathione S Transferase from pGEX-3X vector in BL21 |
| Country | : | India |
| Authors | : | Apurva Tathe |
Abstract:Glutathione S Transferase(GST) is an enzyme of a multi gene family which is involved in reducing oxidative damage to cells and detoxification of Xenobiotic compounds and plays critical role in life processes. The entire work was completely qualitative and the objective of my work was to deal with the induction, extraction and purification of the GST fusion protein from pGEX 3X vector.In order to achieve high degree of transformed cells,the E.Coli BL21 host strain was made competent using 0.1M CaCl2 and adding of pGEX 3X vector into host made it transformed.
[1]. David L. Eaton and Theo K. Bammler, Concise Review of the Glutathione S- Transferases and their significance toToxicology, Toxicological sciences 49, 1999,156-164.
[2]. Philip J. Sherratt and John D. Hayes, Enzyme systems that Metabolise Drugs and Other Xenobiotics
[3]. J D Hayes and D J Pulford , The glutathione S-transferase supergene family: regulation of GST and contribution of the isoenzymes to cancer chemoprotection and drug resistance, Critical Reviews in Biochemistry and Moecular. Biology,30,1995, 445-600.
[4]. David L. Nelson and Michael M. Cox, Lehninger Principle of Biochemistry (Newyork,W.H. Freeman and Company,2008).
[5]. Jeremy M. Berg, John L.Tymoczko and Lubert Stryer, Biochemistry (W.H. Freeman and Company,).
[6]. Robert K. Murray, Daryl K. Granner, Peter A. Mayes and Victor W. Rodwell, Harper's Illustrated Biochemistry (NY, McGraw-Hill Companies,2003).
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Comparative Study of The Antioxidant Activities of Monodora Myristica And A. Sceptrum on Protein And Lipid Levels of Diabetic-Induced Rats. |
| Country | : | Nigeria |
| Authors | : | Nwankwo, P. O. |
Abstract: Diabetes is known to involve oxidative stress and changes in lipid metabolism. Many secondary plant metabolites have been shown to possess antioxidant activities improving the effects of oxidative stress on diabetes. Wistar albino rats (male and female) weighing 160g – 200g were induced with diabetes by administration of streptozotocin (50mg/kg body weight) intraperitoneally. This study evaluated the antihyperlipidaemic effect of methanolic extracts of Aframomum sceptrum and Monodora myristica in comparison with vitamin E (a known antioxidant) in the streptozotocin-induced diabetic rats. Marked increase in plasma and liver levels of total protein, triacylglycerol, total cholesterol, low density lipoprotein cholesterol and decreased level of high density lipoprotein cholesterol were observed in the streptozotocin-induced diabetic rats.
[1]. Karachalias, N., Babaei, J. R., Rabbani, N. and Thonalley, P. J. (2010). Increased protein damage in renal glomeruli, retina, nerve, plasma and urine and its prevention by thiamine and benfotiamine therapy in a rat model of diabetes. Diabetologia, 53 (7): 1506-1516.
[2]. Henzen, C. (2012). Monogenic diabetes mellitus due to defects in insulin secretion. Swiss Medical Weekly, 142: W13690.
[3]. Bartelt, A., Merkel, M. and Heeren, J. (2012). A new, powerful player in lipoprotein metabolism: brown adipose tissue. Journal of Molecular Medicine, 90: 887-893.
[4]. Pu, L. J., Zhang, R. Y., Du, R., Shen, Y., Zhang, Q., Yang, Z. K., Chen, Q. J. and Shen, W. F. (2013). Glycation of apoprotein A-1 is associated with coronary artery plague progression in type 2 diabetic patients. Diabetes Care, 36 (5): 1312-1320.
[5]. Ann, S. B. (2012). Obesity and sedentary life-style: Risk for cardiovascular disease in women. Texas Heart Institute Journal, 39 (2): 224-227.
[6]. Akinmoladun, A. C., Obutor, E. M. and Farombi, E. O. (2010). Evaluation of antioxidant and free radical scavenging capacities of some Nigerian indigenous medicinal plants. Journal of Medicinal Food, 13 (2): 444-451.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | High prevalence of metabolic syndrome among competitive exam appearing students – An observational study from Guntur city, Andhra Pradesh |
| Country | : | India |
| Authors | : | NRVK Swamy || GDV Satya Murthy || M. Siva Durga Prasad Nayak |
Abstract: Introduction: Metabolic syndrome is a pool of risk factors which are indicators of future development of cardiovascular disease and type 2 diabetes mellitus in adults. Stress is strongly associated with metabolic syndrome. Adolescents are more prone to academic stress and thus to metabolic syndrome also. Aim& Objectives: To determine prevalence of metabolic syndrome among the adolescent students appearing for competitive exams. Methods: A comparative descriptive study was conducted among the intermediate students of 16-19 years age group. Students were selected randomly from students residing at hostel in Sri Chaitanya junior college, Guntur.
[1]. Malik S, Wong ND, Franklin SS, Kamath TV, L'Italien GJ, Pio JR, Williams GR 2004. Impact of the metabolic syndrome on mortality from coronary heart disease, cardiovascular disease, and all causes in United States adults. Circulation 110:1245–1250
[2]. Laaksonen DE, Lakka HM, Niskanen LK, Kaplan GA, Salonen JT, Lakka TA 2002. Metabolic syndrome and development of diabetes mellitus: application and validation of recently suggested definitions of the metabolic syndrome in a prospective cohort study. Am J Epidemiol 156:1070–1077
[3]. Rosmond R, Dallman MF, Bjorntorp P. Stress-related cortisol secretion in men relationships with abdominal obesity and endocrine, metabolic and hemodynamic abnormalities. J Clin Endocrinol Metab 1998;83:1853–9.
[4]. Raikkonen K, Hautanen A, Keltikangas-Jarvinen L. Association of stress and
[5]. depression with regional fat distribution in healthy middle-aged men. J Behav Med 1994;17:605–16.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Role of Serum Copper Ans Zinc In Pathogenesis of Psoriasis |
| Country | : | India |
| Authors | : | Dr.Margit Gajjar || Dr.H.B.Sirajwala || Dr.Dipti Gajjar || Dr.Ipsa Pandya |
Abstract: Psoriasis is a hyper proliferative cutaneous disease of multifactorial etiologies: genetic background, environmental factors, vascular and immune system disturbances 1.Typical psoriatic lesions are erythomatous papules which form plaques characterized by sharp borders and increased scaling.
[1]. Nagat Sobhy Mohamad: Trace elements homeostatic imbalance in mild and severe psoriasis: a new insight in biomarker diagnostic value for psoriasis. Our Dermatol Online. 2013; 4(4): 449-452
[2]. Estabraq ARK Alwasiti, Wasan T Al-Rubayee, Tammimy:Serum Copper, Zinc and Oxidative Stress in Patients with Psoriasis, Al-Nahrain College of Medicine ISSN 1681-6579, IRAQI J MED SCI, 2011; VOL.9 (2).
[3]. Carl A Burtis, Edward Ashwood, David E Burns: Teitz Textbook of Clinical Chemistry and Molecular diagnostics;Fifth edition, Chapter 31:Vitamin and trace elements;948-964.
[4]. National committee for Clinical Laboratory Standards Approved guidelines: Control of pre-analytical variation in trace element determination 1997; 17:1-30.
[5]. Siva ME, Subramanian KN: Kinetic models of trace element and mineral metabolism during development. CRC, 1995, Boca Raton, pp 159–70.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | "A Study on Coagulation Profile in Pregnancy Induced Hypertension Cases" |
| Country | : | India |
| Authors | : | Dr.Asiya Naaz MD || Dr.Suhasini Padugupati MD|| Dr.DVHS Sarma MD || P.Sushma MSc |
Abstract:Pregnancy is a physiological process in women but it may be associated with certain risks to the
health and life of both the mother and child. Hypertensive disorders complicating pregnancy are common and
form one of the deadly triad along with haemorrhage and infection that results in maternal and perinatal
morbidity and mortality. Pregnancy can induce hypertension in normotensive women or aggravate already
existing hypertension or appears for the first time during pregnancy. The identification of this clinical entity and
effective management play a significant role in the outcome of pregnancy, both for the mother and the baby. In
the developing countries with uncared pregnancy, this entity on many occasions remains undetected till major
complications supervene.
[1]. Darnforth editor Obstetrics & Gynaecology (1999), Lippincott Williams andWilkins, New York, 309-327.
[2]. Cunningham FG, MacDonald PC & Gant NF (1989). Hypertensive disorders in pregnancy. In: Cunningham FG, MacDonald PC &
Gant NF (Editors), Williams Obstetrics. Prentice-Hall, Norwalk, 653-694.
[3]. Andrea G. Witlin, and Sibai B M. Hypertension in pregnancy: current concepts of preeclampsia. Ann Rev Med 1997 Feb; 48: 115-
127.
[4]. J.G.L. Ramos, S.H. Martins-Costa, J.B. Kessler, C.A. Costa and E. Barros.Calciuria and preeclampsia. Braz J Med Biol Res, 1998,
31(4) 519-522.
[5]. D.C Dutta Text Book of Obstetrics; 6th edition.
[6]. William's Obstetrics; Cunningham F, Gray; 4th edition.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | The Growth of Microalgae in Shrimp Hatchery: Impact of Environment on Nutritional Values |
| Country | : | India |
| Authors | : | G. RAJ KUMAR, (PhD) || Prof. D.E. BABU |
Abstract: India is the second-largest aquaculture producer in the world. Like the largest producer, China, India's Aquaculture is dominated by carp production: about 80% of India's aquaculture production is composed of carps of Indian and Chinese origin. In the last 20 years, carp production has intensified in several parts of India. The challenges ahead include feed cost management which needs to be achieved through optimization of feed ingredients, and increasing farm gate price of fish though efficient post-harvest preservation and marketing techniques. Also species diversification to include higher value fish in farming will pave way for widespread feed-based farming and bring better returns to the farmer.
[1]. GP Kumaraswamy Acharya and kkaumudi menon, A New and simple medium for phytoplankton culture, seaweed, Res. Utila, 16(1 & 2) pp 161-166(1993).
[2]. Sushanth Vishwanath Rai and Madaiah Rajashekhar* Effect of ph, salinity and temperature on the growth of six species of marine phytoplankton, J. Algal Biomass Utln. 2014, 5 (4): 55-59.
[3]. Vishwanath Patil , Fatty acid composition of 12 microalgae for possible use in aquaculture feed, Aquacult Int (2007) 15:1–9.
[4]. Arnaud Muller-Feuga,The role of microalgae in aquaculture: situation and trends, journal of applied phycology, 12(3-5),pp527-534. 2000.
[5]. M.R. Brown, S.W. Jeffrey, J.K. Volkman, G.A Dunstan,Nutritional properties of microalgae for mariculture, Aquaculture 151 (1997) 315-331.
[6]. Ivaylo Sirakov, Katya Velichkova, Stefka Stoyanova, Yordan Staykov,The importance of microalgae for aquaculture industry. Review. International Journal of Fisheries and Aquatic Studies 2015; 2(4): 81-84.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Role of serum trace elements magnesium, copper and zinc, level in Libyan patients with bronchial asthma |
| Country | : | Egypt |
| Authors | : | Khaled S. Al-salhen || Sayed A. M. Mahmoud || Salmah M.Omran || Safaa K. Mohammed |
Abstract: The present study compared the levels of serum Mg, Cu and Zn between the adult with asthma and healthy volunteer in order to evaluate the associations of these nutrients with asthmatic patients. Thirty-three adults patients of both (19 males, 14 females) with the median age 39 years with bronchial asthma, who were admitted by the Department of Pulmonary Diseases of Shat and Alkyfaya Hospitals, were included in the study. Thirty-three adult patients of diagnosed bronchial asthma of both sexes (19 males and 14 females) attending Pulmonary Diseases Care Unit at Shat and Alkyfaya Teaching Hospital in El-Beida and Benghazi Cities and 31 healthy volunteers (17 males, 14 females) were studies.
[1] N. Lukác, P. Massányi, Effects of trace elements on the immune system. Epidemiol. Mikrobiol. Imunol. 56, 2007, 3-9.
[2] Q. Shazia, Z. Mohammad, I. Taibur Rahman, U. Hossain, Correlation of oxidative stress with serum trace element levels and antioxidant enzyme status in beta thalassemia major patients: A review of the literature. Hindawi publishing corporation, anemia, 7, 2012, 20-23.
[3] N.N. Jarjour, W.J. Calhoun, Enhanced production of oxygen radicals in asthma. . J. Lab. Clin. Med. 123, 1994, 131-137.
[4] D.J. Fraenkel, S.T. Holgate, Etiology of asthma: Pathology and mediators , In: C.W. Biermann, D.S. Pearlman, G.G. Shpiro, W.W. Busse. Allergy, Asthma and Immunology from Infancy to Adulthood. WB Sauners Company., Philadelphia, 1996.
[5] A. Kocyigit, F. Armutcu, A. Gurel, B. Ermis, Alterations in plasma essential trace elements selenium, manganese, zinc, copper, and iron concentrations and the possible role of these elements on oxidative status in patients with childhood asthma. Trace Elem Res 97, 2004, 31-41.
[6] F. Kelly, I. Mudway, A. Blomberg, A. Frew, T. Sandstrom, Altered lung antioxidant status in patients with asthma. Lancet. 354, 1999, 482.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Preservative potentials of crude bacteriocins produced by Lactobacillus tucceti CECT 5920 and Lactobacillus mindensis TMW on Escherichia coli 0157:H7 and Staphylococcus aureus NCTC 8325 |
| Country | : | Nigeria |
| Authors | : | OBI, C. N. |
Abstract: Preservation potentials of lactic acid bacteria (LAB) isolated from traditional fermented foods were evaluated by inoculating 0.1ml aliquots of appropriate dilution of the samples on De Man Rogosa Sharpe (MRS) agar fortified with 50mg of nystatin for the isolation of LAB. The LAB isolates recovered were screened for bacteriocin production by the Agar Well Diffusion assay and two best bacteriocin producers characterized by (GTG)5-PCR and 16S rDNA protocol as Lactobacillus tucceti CECT 5920 and Lactobacillus mindensis TMW. They were tested for their bio-preservative potentials against Staphylococcus aureus NCTC 8325 and Escherichia coli 0157:H7 in "Five Alive" and La casera fruit drinks. L. tucceti and L. mindensis had the same level of bacteriocin production and antimicrobial activity (P<0.05).
[1]. AOAC 2005. Association of Official Analytical Chemists. Official Methods of Analysis 15th Edition (Helrick, K.ed.). AOAC, Arlington, Virginia.
[2]. Adesokan, I. A., Odetoyinbo, B. B and Okanlawon, B. M. (2009) Optimization of Lactic Acid Production by Lactic Acid Bacteria Isolated from
[3]. Some Traditional Fermented Food in Nigeria. Pakistan Journal of Nutrition 8 (5): 611-615.
[4]. Bizani, D and Brandelli, A (2002) Characterization of bacteriocin produced by a newly isolated Bacillus sp. strain 8A, J. Appl. Microbiol. 93: 512–519.
[5]. Bromberg, R., Moreno, I., Zaganini, C. L., Delboni, R. R and De Oliveira, J (2004) Isolation of bacteriocin producing lactic acid bacteria from meat and meat products and its spectrum of inhibitory activity, Braz. J. Microbiol. 35:137–144.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Effect of Techniques in Preparing VCO Nanoparticles |
| Country | : | Libya |
| Authors | : | Farah Diana Ariffin || Rosnani Hasham || Mariani AbdulHamid || Ahmad M. Eid || Aref T. Mohamed || Eseldin I. Keleb || Assad Elmahgoubi || Yosef S. Issa || Nagib A. Elmarzugi |
Abstract: This study presents the effects of two techniques in preparing solid lipid nanoparticles (SLN) incorporated with virgin coconut oil (VCO). VCO-SLNs were prepared using two different techniques which were high pressure homogenizer (HPH) and probe sonicator. HPH was done at 400 bars for six cycle and for probe sonicator, three different amplitudes were used; 30, 50 and 70. The VCO-SLNs were characterized with respect to particle sizes, polydispersity index (PDI) and size distribution by intensity. VCO-SLNs using HPH had an average diameter of 289.4 and PDI of 0.680. The average diameter and PDI for probe sonicator with amplitude 30, 50 and 70 were 113.4 and 0.336, 345.4 and 0.441, and 276.7 and 0.386, respectively. This study demonstrates that different techniques in preparation of SLNs can manipulate the physicochemical properties of SLNs especially particle size and PDI.
Keywords: Nanoemulsion, Nanoemulgel, Emulsifying method, Liposomes, Thixotropic, Hydrogel
[1] A. Saupe, T. Rades, Solid lipid nanoparticles inM.R. Mozafari (Ed), Nanocarrier Technologies: Frontiers of Nanotherapy (Netherlands: Springer 2006) 41-50.
[2] S.A. Wissing, R.H. Müller, Cosmetic applications for solid lipid nanoparticles (SLN), International Journal of Pharmaceutics, 254 (1), 2003, 65-68.
[3] S. Utreja, N. Jain, Solid lipid nanoparticles in N.K. Jain (Ed), Advances in controlled and novel drug delivery(New DelhiCBS Publishers2001) 408-425.
[4] R.H. Müller, M. Radtke, S.A. Wissing, Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations, Advanced Drug Delivery Reviews, 54, 2002, S131-S155.
[5] W. Mehnert, K. Mäder, Solid lipid nanoparticles: production, characterization and applications, Advanced Drug Delivery Reviews, 47(2), 2001, 165-196.