Version-1 (Jan–Feb 2016)
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| Paper Type | : | Research Paper |
| Title | : | Biochemical and Organoleptic Study of the Mahua Flower and Mahua Flower Wine. |
| Country | : | India |
| Authors | : | Dr. Jyoti D Vora || Padma Srinivasan || Tejasa Singnurkar |
Abstract: Mahua (Madhucaindica) is a large, deciduous tree native to the northern and central parts of India. Tribal populations across the country value the flowers of this tree as a source of food. The mahua flowers are one of the most underutilized nutrient sources due to their extremely rare consumption among the Indian populace. The flowers are nutritionally balanced as a rich source of carbohydrates, proteins, dietary fibers in addition to minerals like iron and calcium. The most interesting attribute of the mahua flowers is their long shelf life after drying. In the dried state these flowers maintain the integrity of nutrients, thus significantly reducing nutrient losses upon storage. Due to all these reasons, exploration of the nutritional profile of the mahua flowers contributes a new perspective to biochemical research.
[1]. C.R. Kothari, Research Methodology Methods and Techniques- 2ndEdition, (New age international (P) Ltd. Publishers, ISBN 81-224-1522-9).
[2]. Sadashivam S, Manickam A, Biochemical Methods- 3rd Edition (New age international (P) Ltd. Publishers. ISBN 81-224-0976-8).
[3]. Aditi Gupta et al, Potential applications of Mahua biomass, Waste and Biomass Valorization, Volume 3 Issue 2, Pages 175-189.
[4]. Fessler JH , Guideline to practical winemaking, P. O. Box 5276, Elmwood Station, Berkelery,CA, 1971.
[5]. Association of Official analytical Chemists (A.O.A.C.), Official methods of analysis, Washington, D. C., U. S. A. 1985.
[6]. Jackson RS Wine Science: Principles and Applications. Academic Press, 1994, page 92.
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| Paper Type | : | Research Paper |
| Title | : | Raised Lipid Profile In Rheumatoid Arthritis- A Risk For CVD |
| Country | : | India |
| Authors | : | Dr. Soobia Karim Ansari || Dr.Geeta Jaiswal |
Abstract: Rheumatoid arthritis(RA) is an autoimmune disorder, which causes chronic inflammation of the joints.Patients with newly diagnosed rheumatoid arthritis have adverse serum lipid profile. We sought to determine the effects of treating rheumatoid arthritis with NSAIDs on these abnormal lipid profile. The study population selected was from the city of Allahabad (U.P.). They were further classified into control group, Consisting of healthy males and females, Group-I Consisting of individual suffering from RA and Group II Consisting of individual suffering from RA and taking anti-inflammatory drugs..In all subjects a blood sample was collected after an overnight fast plain vials are used for the determination of lipid profile.Of the Blood lipid profile studied i.e.
[1]. Krishnamurthy N.V., Seethalakshmi R., Poornima K. Murthy &Venkatesh Rao K.L.(1988).: Study on the effect of Tab Arthnex Forte on rheumatoid arthritis and osteSoarthritis. The Antiseptic.Vol.100 No. 8,P, 305-307.
[2]. Maradit-Kremers H,Crowson CS,Nicola PJ,Ballman KV,Roger VL,Jacobsen SJ,Gabriel SE. Increased unrecognized coronary heart disease and sudden deaths in rheumatoid arthritis: a population-based cohort study.Arthritis Rheum.2005;52(2):402-411.
[3]. Nicola PJ,Maradit-Kremers H, Roger VL,Jacobson SJ,Crowson CS,Ballman KV,Gabriel SE. The risk of congestive heart failure in rheumatoid arthritis: a population-based study over 46 years.Arthritis Rheu.2005:52(2):412-420.
[4]. Full LE,Ruisanchez C,Monaco C. The inextricable link between artherosclerosis and prototypical inflammatory diseases rheumatoid arthritis and systemic lupus erythematatosus.Arthritis Res Ther.2009;11(2):217.
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| Paper Type | : | Research Paper |
| Title | : | Phytochemical Comparative Screening of Aqueous Extracts of the Leaves, Stem barks, and Roots of Hura crepitans (L) using GC – FID. |
| Country | : | Nigeria |
| Authors | : | Adindu, Eze Azubuike || Elekwa, Iheanyichukwu || Ogwo Joseph Ikedi |
Abstract: The phytochemical screening of the aqueous extracts of leaves,stem bark and roots of Hura crepitans were analysed using the Gas Chromatography – Flame Ionization Detector (GC – FID) method. The various parts of the plants were collected and air-dried under shade for one month after which they were sent for analysis. All the various parts showed varying amounts of phytochemicals: Flavonoids, Carotenoids, Sterols, Terpenes, Alkaloids, Saponins and Phenolic acids.
[1]. Abidi, S.L., (2001). Chromatographic analysis of plant sterols in foods and vegetable oils. Journal of Chromatography 935:173-201.
[2]. Adedire, C.O. and Ajayi, O.E., (2003). Potential of Sandbox, Hura crepitans seed Oil for Protection of Cowpea Seeds from CallosobruchusMaculatusFabricius (Coleoptera: Bruchidae) Infestation. Journal of Plant Diseases and Protection 110 (6):602-610.
[3]. Bajaj, Y.P.S., (1988). Biotechnology in Agriculture and Forestry. Springer-Verlag Berlin Heidelberg New York. Pp 2-80.
[4]. Barbieri,L; Falasca, A; Franceschi,C; Licastro,F; Rossi,F and Stripe, F., (1983). Purification and properties of two lectins from the latex of Euphorbiaceous plants Hura crepitans and Euphorbia characias. Biochem. J.215:433-439
[5]. Boutanaev, A. M; Moses, T; Zi, J; Nelson, D. R; Mugford, S. T; Peters, R. J; Osbourn, A, (2015). Investigation of terpene diversification across multiple sequenced plant genomes. Proc. Natt. Acad. Sci. 112 (1): E81 – E88.
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| Paper Type | : | Research Paper |
| Title | : | Experimental (FT-IR, UV-visible, NMR) spectroscopy and molecular structure, global reactivity parameter, Fukui function, NBO, NLO, HOMO-LUMO, MESP and QTAIM analyses of Abacavir using density functional theory |
| Country | : | India |
| Authors | : | Shashi Kumar Gangwar || Ambrish Kumar Srivastava || R.B. Singh |
Abstract: In the present work, FT-IR, 1H NMR, UV-visible spectra of [(1S,4R)-4-[2-amino-6-(cyclopropylamino) purine-9-yl]cyclopent-2-en-1-yl] methanol (Abacavir) have been analyzed at the room temperature. The equilibrium geometry, harmonic vibrational frequencies, infrared intensities, 1H-NMR and bonding properties have been calculated by density functional theory employing B3LYP hybrid exchange-correlation functional with complete relaxation in the potential energy surface. The fundamental vibrational modes have been assigned on the basis of the potential energy distribution.
[1]. B.D. Cheason, M.I. Keating, W. Plunkett (1997) Nucleoside analogous in cancer therapy. Dekker, New York.
[2]. P. Sarmah, R.C. Deka Anticancer activity of nucleoside analogues J Mol Model (2010) 16:411-418.
[3]. J. Jacquesn, L.B.H.Lorraine, O.France, Sylvain BAR, Herriette G (2003) cancer Chemother Pharmacol 52:497-506.
[4]. P. Sarma , C.Ramesh J. Deka J Mol Model (2010) 16:411-418.(doi 10.1007/s00894-009-0551-9.
[5]. A Carr, C Workman, D.E. Smith, J Hoy, J. Hudson Jama, 2002.
[6]. F. Fermandez , X. Garcia-Mera,Morales M. Mera Morales, Rodriguez-Borges JE(2001) Synthesis 2:239-242.
[7]. F. Abad, Alvarez. F. Alvarege, F. Fermandez, X.Garcia-Mera, J.E. Rodriguez-Borges (2001) Nucleosides. Nucleic Acids 20:1127-11228.
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| Paper Type | : | Research Paper |
| Title | : | Effect of mow procedure on physiological and biochemical properties of blood in sheep |
| Country | : | Iraq |
| Authors | : | Moaead A.A tweech || Abbas .G. Hamad || Areej Ghazi || Ali .N || marwa,A,A ||| mayes .A |
Abstract:In this study18 female sheep aged between 20-25 months was used in this experiment in alqasim
city .the mow procedure was performed in a closed area using traditional method with shearers.
The aim of study was to evaluate the stress response and the oxidant antioxidant balance against shearing
process in sheep. Blood malonaldldhyde (MDA)and glutathione (GSH)concentration , were measured in 18
female sheep one day before a traditional mow procedure. The study show increase of respiration and heart
rate . Circulating MDA concentrations were dramatically increased compared to initial values after shearing
whereas GSH concentrations were significantly depressed .the variations of other biochemical parameters
were not significant .these results demonstrate that MDA and GSH are the most powerful markers for
evaluating the oxidant /antioxidant status and that mow was a stressful situation leading to an oxidative stress .
Key word: sheep, mow, cholesterol
[1]. Y. ALEKSIEV.2011. Feed intake and milk yield responses to shearing in Pleven Blackhead sheep with different levels of
production Bulgarian Journal of Agricultural Science, 17 (No 5) 2011, 673-679Agricultural Academy
[2]. ARNEMOJ.M.NEGARDT,SOLIN.E.Chemicsl capture of free-ranging red deer (cervus elaphus)with medetomidine –ketamine
rangirer 1994,14,123-127-.
[3]. GLENN J,.LARSON S,. MARKEGARD G,.NELSON A,.PHILLIPS R,.PRICE.E,.STULL C.;Sheepcare practices
BEALLG(ed),Sheep workgroup animal care series ,university of California cooperative extension davis 1996,Retrived 11.09.2007
from http://www.vetmed.ucdavis.edu /vetext/INF-SH-CarePrax.pdf.
[4]. CARGANGIU V.,VACCA G.M.,PARMEGGIANI A,MURA M.C,.PAZZOLA M,.DETTORI M.L,.BINI P.P,;The effect of
shearing procedures on blood levls of growth hormone and other stress haematochemical parameters in sarda sheep Animal
2008,2,606-612..
[5]. HARGREAVES A.L.,HUTSON G.D,;Changes in heart-rate ,plasmacortisol and haematocrit of sheep during ashearing procedure
appl.Anim.behav.Sci,.1990,26,19-101.
[6]. KATZ D,.MAZOR D,.DVILANSKY A,.MEYERSTEIN N,;Effect of radiation on red cell membrane and intra cellular oxidative
defense system .free red Res.,1996,24,199-204.
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| Paper Type | : | Research Paper |
| Title | : | O- cresol Biodegrdation by Bacillus badius D1 isolated from Salt Lake Lonar (MS), India. |
| Country | : | India |
| Authors | : | Vasudeo D. Sarwade |
Abstract: Cresols are known to be dominant lethal mutations in germ cells in male mice. Cresols may persist in extremely oligotrophic water bodies, and/or those under anaerobic conditions, such as in some sediments and groundwater aquifers The Strain Bacillus badius D1 was employed for biodegradation of o-cresol at higher concentration isolated from Lonar Crater Lake Buldana (MS), India. It has degraded 350 mg/L concentration within 30 hr. The optimum pH and temperature observed was 9.00 and 32 0C respectively. The intermediates of catabolism were trapped as salicylic acid and catechol. This organism can be employed for bioremediation at polluted site or in bioreactors.
Key Words: Bacillus badius, alkaliphile, o-cresol, biodegradation
[1]. OVERVIEW, Potentiall Forr Human Exposure, pp- 147-186, 2006.
[2]. OECD SIDS m- / p-CRESOL, pp-1-337, 2003
[3]. V.D. Sarwade, Biodegradation of Benzidibe by Alkaliphilic Strain Bacillus badius D1, IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-ISSN: 2319-2402, Volume 8, Issue 11 PP 73-80, 2014.
[4]. V. D. Sarwade1, K.R. Gawai, Biodegradation of phenol by alkaliphiic Bacillus badius D1, IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-ISSN: 2319-2402,p- ISSN: 2319-2399.Volume 8, PP 28-35, 2014
[5]. P.Balamurugan, B.Preetha, Statistical Evaluation of Biodegradation of O-Cresol using Aspergillus fumigatesInternational Journal of ChemTech Research CODEN (USA): IJCRGG ISSN : 0974-4290 , Vol.6, No.12, pp 4934-4939, 2014
[6]. Rafael G. Dolatto, Iara Messerschmidt, Determination of Phenol and o-Cresol in Soil Extracts by Flow Injection Analysis with Spectrophotometric Detection, J. Braz.Chem. Soc, Vol. 23,(5), pp - 970-976, 2012.
[7]. Omura T. and Sato T.R., The Carbon Monoxide-binding Pigment of Liver Microsomes, J. Of Biol. Chem, 239, pp - 2370-2378, 1964
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| Paper Type | : | Research Paper |
| Title | : | "Ethnomedicinal Studies Of Deori Tribes Of Bihpuria Sub Division, Lakhimpur District, Assam" |
| Country | : | India |
| Authors | : | Bharat Hazarika || Debajit Dutta |
Abstract: Ethno medicinal knowledge is very ancient. It provides information regarding the traditional uses of plants which played a key role in the health systems of different ethnic groups living in remote areas of North East India. Ethnomedicinal study of the Deori Community of Bihpuria sub division, Lakhimpur district Assam was done during 2013/2014. In the present study 60 species of plant growing wild in Rural, semi forest, cultivated frield and cultivated garden which are commonly used by people of this region. Deori community utilized Ethno medicinal plants for the treatment of diseases and disorders like diarrhoea, dysentery, fever, headache, skin diseases, boils, and blisters, rheumatism and gout, piles, jaundice, ophthalmic diseases, toothacte, cut and wounds, cough cold, asthma and other common diseases. The present paper reports the some common plant species and their scientific name, local name, family, distribution, parts used and medicinal used for curing diseases.
Keywords: Ethno medicine, Bihpuria, North Eastern area
[1]. Jain S. K. 1967. Ethno botany its scope and study. Bull Indian mus. 2(1):39-43
[2]. Jain S. K. 1986. Ethno botany. Interdisciplinary Sci. Rev. 11(3): 285-292
[3]. Jain S. K. 1987. A manual of Ethno botany. Sci. Publ., Jodhpur.
[4]. Kanjilal U. C., Das A., Kanjilal P.C. and De R.N. (1934-1940). Flora of Assam. Vol 1-5. Govt of Assam, Shillong (Vol. 5 by N.L Bor). (Reprinted 1982)
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| Paper Type | : | Research Paper |
| Title | : | Evaluation of Tumor necrosis factor-alpha in patients with Coronary Artery Disease |
| Country | : | India |
| Authors | : | Laikangbam Shaini || Ahilya P || Th. Sachin Deba Singh || Soumyadip Sarma || Konsam Photan Singh || Rupak Das || Suman Deb Nath |
Abstract: Tumor necrosis factor-α (TNF-α) is a multifunctional circulating cytokine derived from endothelial and smooth muscle cells as well as macrophages associated with coronary atheroma. A large number of genetic and epidemiological studies have identified TNF-α as a risk factor for atherosclerotic disease such as Coronary Artery disease and stroke. Based on our knowledge, this is the first study to estimate serum TNF-α concentration in Manipur.
[1] Longo DL, Fauci AS, Kasper DL, Hauser SL and Jameson JL, Loscalzo editors. Harrison's Principle of Internal Medicine, 18th ed. USA: McGraw Hill Co; 2012.
[2] Rosa MS, Pinto M. Cytokines. In: Burtis CA, Ashwood ER, Bruns DE, editors. Teitz textbook of clinical chemistry and molecular diagnostics. 4th ed. St Louis: Saunders Elsevier;2006.p.645-744.
[3] Weis M, Schlichting SC, Engelman EG, Cooke JP. Endothelial determinants of dendritic cell adhesion and migration: new implications for vascular disease. ArteriosclThrombVasc Biol 2002;22(11):1817-23.
[4] Bruunsgaard H, Skinhoj P, Pederson AN, Schroll M, Pederson BK. Ageing, tumor necrosis factor-α(TNF-α) and atherosclerosis. Clin Exp Immunol 2000;121(2):255-60.
[5] Ross R. Atherosclerosis: an inflammatory disease. N Engl J Med 1999;340:115-26.
[6] Brett J, Gerlach H, Nawroth P, Steinberg S, Godman G, Stern D. Tumor necrosis factor/cachectin increases permeability of endothelial cell monolayer by a mechanism involving regulatory G proteins. J Exp Med 1989;169(6):1977-91.
[7] Collins T, Read MA, Neish AS, Whitley MZ, Thanos D, Maniatis T. Transcriptional regulation of endothelial cell adhesion molecules: NF-kappa B and cytokine-inducible enhancers. FASEB J 1995;9(10):899-909.
[8] Grundy SM,Brewer HB Jr, Cleeman JI, Smith SC Jr, Lenfant C. Definition of the metabolic syndrome: Report of the National heart, lung, and blood institute/American Heart Association conference on scientific issues related to definition. Circulation 2004;109(3):433-38.
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| Paper Type | : | Research Paper |
| Title | : | Interference of serum indices in the analyzer function |
| Country | : | India |
| Authors | : | Usha Adiga || Dr. Usha Sachidananda Adiga |
Abstract: Analyzer has the capability to detect hemolysis, icterus, and lipemia in samples and produces semi quantitative unit less index values for hemoglobin, bilirubin, or intralipid (so-called serum indices), but these serum index functions are not intended for diagnostic purposes. With increasing amounts of the specific interfering compounds, increasing index values are generated, which are linearly correlated with the amount of interferent. Aim of our study is to find the influence of serum indices on the functioning of the analyzer. Methodology: Blood glucose estimation was done for twenty samples. Serum indices analysis was done for the same set of samples along with glucose in the next run. Time taken for analysis was noted. Results: The time taken for the estimation of glucose by automated chemistry analyzer is 9.27 minutes. When analyzed with serum indices, time required is 9.45 min.
[1]. M,Plebani and P, Carraro, Mistakes in a stat laboratory: types and frequency. Clin Chem, 43(8 pt 1),1997,1348–51.
[2]. D.L.Witte , S.A.VanNess , D.S.Angstadt , B.J.Pennell, Errors, mistakes, blunders, outliers, or unacceptable results: how many? Clin Chem,43(8), 1997,1352–6.
[3]. M.H.Kroll, R.J.Elin, Interferences with clinical laboratory analyses. Clin Chem,40 (11 pt 1),1994,1996–2005.
[4]. H.Behrendt, Chemistry of erythrocytes. Springfield, IL: Charles C Thomas,1957,227.
[5]. W.T.Caraway, Chemical and diagnostic specificity of laboratory tests, effect of hemolysis, lipemia, anticoagulants, medications, contaminants and other variants. Am J Clin Pathol,37, 1962,445–64.
[6]. Giuseppe Lippi , Paola Avanzini , Daniele Campioli. Systematical assessment of serum indices does not impair efficiency of clinical chemistry testing: A multicenter study, Clinical Biochemistry, 46(13-14),2013 ,1281–1284.
[7]. G.Lippi , G.C.Guidi, Risk management in the preanalytical phase of laboratory testing, Clin Chem Lab Med , 45(6),2007, 720–7.
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| Paper Type | : | Research Paper |
| Title | : | Detection of Porcine Rotaviruses Group A in Free-ranging and Farm Pig Population of North East India |
| Country | : | India |
| Authors | : | Sushmita Das || P.J.Handique |
Abstract: Piglet diarrhea caused by rotavirus is one of the major threats leading to serious economic losses in swine industry of the North East India. Thus, there is a dire need of rapid and sensitive detection techniques in identifying the causative agent for control and containment of the disease. In our study, porcine rotavirus group A was detected using the gold standard method, native RNA PAGE followed by reverse transcription (RT) PCR in 208 fecal samples from diarrheic piglets of 0-6 months of age reared under different management systems in Assam and Tripura states of North East India. The study also indicated that the use of RNA PAGE followed by RT PCR of VP4 as well as VP7 gene is more confirmative over PAGE alone. In addition, the present investigation revealed that the use of RNA PAGE is advantageous in knowing the group prevalent amongst the circulating rotavirus strains of a region whereas RT PCR besides acting as an effective tool in detection of porcine group A rotavirus, comes out as an efficient means for further genetic studies of the same.
Keywords: Native RNA PAGE, North East India, Piglet diarrhea, Porcine rotavirus Group A, RT PCR
[1] R. Smitalova, L. Rodak, I. Psikal, and B. Smid, Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses, Veterinarni Medicina, 51(5), 2006, 288-295.
[2] S. E. Midgley, K. Banyai, J. Buesa, N. Halaihel, C. K. Hjulsager, F. Jakab, J. Kaplon, L. E. Larsen, M. Monini, M. Poljsak-Prijatelj, P. Pothier, F. M. Ruggeri, A. Steyer, M. Koopmans, and B. Bottiger, Diversity and zoonotic potential of rotaviruses in swine and cattle across Europe, Veterinary Microbiology, 156, 2012, 238-245.
[3] B. R. P. Barbosa, N. T. C. G. Bernardes, L. A. R. Beserra, and F. Gregori, Molecular characterization of porcine group A rotavirus NSP2 and NSP5/6 genes from Sao Paulo State, Brazil, in 2011/12, The Scientific World Journal, doi:10.1155/2013/241686, 2013.
[4] A. Z. Kapikian, Y. Hoshino, and R. M. Chanock, Rotaviruses fields- virology (London: Lippincott Williams & Wilkins, 2001) 1787-1833.
[5] M. K. Estes, D. Y. Graham, and D. H. Dimitrov, The molecular epidemiology of rotavirus gastroenteritis, Progress in Medical Viroogy, 29, 1984, 1-22.