Version-1 (Jan–Feb 2016)
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| Paper Type | : | Research Paper |
| Title | : | In vitro Physico-chemical, Phytochemical and Fluroscence Assessment of Mucuna sps. |
| Country | : | India |
| Authors | : | S. N. Murthy || S. Sangvikar || M. M. Malgaonkar || C. Sharma || Y. R. Kulkarni |
Abstract: Mucuna is commonly called as "Kapikacchu', which is known to have various medicinal properties and being used in Ayurveda since ages. Medicinal plants have bioactive compounds which are used for curing various diseases. The present investigation is aimed to screen the various bioactive compounds present in seeds of Mucuna pruriens (L.) DC., Mucuna cochinchinensis (Lour.)A. Chev. black seeds variety and Mucuna cochinchinensis (Lour.) A. Chev. white seeds variety. In the present study, an attempt was made to analyse and evaluate the presence of various phytoconstituents as well as fluorescent characteristics of seeds of Mucuna sps. Along with this, during the research work, physicochemical parameters were also determined.
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[3]. Eilittä, M., Bressani, R., Carew, L.B., Carsky, R.J., Flores, M., Gilbert, R., Huyck, L., St-Laurent, L. and Szabo, N.J. (2002). Mucuna as a food and feed crop: an overview. In: Mucuna as a Food and Feed: Current Uses and the Way Forward. Ed. By Flores M, Eilittä M, Myhrman R, Carew L, Carsky R, Workshop held April 26-29, 2000 in Tegucigalpa, Honduras. CIDICCO Honduras. pp. 18-46.
[4]. Janardhanan K. and Lakshmanan K.K. (1985). Studies on the pulse, Mucuna utilis. Chemical composition and anti-nutritional factors. J. Food Sci. Tech. 22: 369-37.
[5]. Mohan, V.R. and Janardhanan, K. (1993). Studies on the Indian tribal pulses. In proceedings of the national seminar on biodiversity: strategies for conservation and future challenges. Department of Botany. Bharathiar University. Ed. by Udaiyan K, Janardhanan K, Manian S, Reddy VRKP. pp. 116-125.
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| Paper Type | : | Research Paper |
| Title | : | Screening and Identification of traits in plant growth promoting rhizobacteria from rhizospheric soils of Persea bombycina |
| Country | : | India |
| Authors | : | Banasmita Das || Hridip Kumar Sarma || Gargee Konwar || Juri Saikia || Jintu Rabha |
Abstract: The rhizosphere is the region of soil that is influenced by root secretions and associated soil microorganisms. Plant growth promotingrhizobacteria (PGPR) are a heterogeneous group of bacteria found in the rhizosphere . Here, an attempt has been made to screen and identify PGPR traits in bacteria isolated and characterized from Perseabombycinarhizospheric soils. Samples were collected from rhizosphere of Som (Perseabombycina) growing in the Regional Botanical Garden, Department of Botany, Gauhati University, Guwahati. 14 pure cultures were obtained in solid Nutient Agar Media. For in vitro screening of isolates for their PGP activities, tests include Indole Acetic Acid Test, quantitative analysis of IAA, production of ammonia (NH3), production of hydrogen cyanide (HCN).
[1]. Anith, K. N., Tilak, K. V. B. R. and Kanuja, S. P. S. 1999. Molecular basis of antifungal toxin production by Fluorescent Pseudomonas sp. strain EM85-a biological control agent. Curr. Sci., 77: 671-677.
[2]. Brick, J.M., Bostock, R.M. and Silverstone, S.E., 1991. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on nitrocellulose membrane. Appl. Environ. Microbiol. 57: 535–538.
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[4]. Corbett, J. R., 1974. Pesticide design. In :The Biochemical Mode Action Pesticides, Academic Press, Inc., London, pp. 44-86.
[5]. Dave, A. and Patel, H. H., 1999. Inorganic phosphate solubilizing soil Pseudomonas. Indian J. Microbiol., 30: 305-310.
[6]. Defago, G., Berling, C. H., Borger, U., Keel, C. and Voisard, C., 1990. Suppression of blackrot of tobacco by a Pseudomonas strain : Potential applications and mechanisms. In: Biological Control Soil Borne Plant Pathogens. Eds. D. Hornby, R. J. Cook and Y. Henis, CAB International, pp. 93-108.
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| Paper Type | : | Research Paper |
| Title | : | Benzyl isothiocyanate production from Salvadorapersica L. callus cultures |
| Country | : | Egypt |
| Authors | : | Ghada A. Hegazi || Nashwa A. El-Hanafy || Zakia A. Abu-Elkheir || Ismail A. Hussein |
Abstract: In vitro production of valuable secondary metabolites is the best suited alternative over traditional field cultivation and chemical synthesis, for mass production of natural pure chemicals more cheaply and rapidly, independently from climate change and negative biotic and abiotic influences on secondary metabolites production in naturally grown plants. Benzyl isothiocyanate (BITC), a major compound in Salvadorapersica L., is one of the naturally occurring isothiocyanates, and has antioxidant and anticancer properties. In this study, an in vitro method was developed for the production of BITC from callus cultures of S. persica, a rare medicinal plant in Egypt. Two types of explants; leaf and stem sections were cultured on Murashige and Skoog (MS) medium, supplemented with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) independently or in combination with kinetin (Kn), for callus induction and mentainance..
[1]. Aftab, F., Akram, S. &Iqbal, J. (2010).Estimation of fixed oils from various explants and in vitro callus cultures of Jojoba (Simmondsiachinensis).Pakistan Journal of Botany, 40 (4), 1467-1471.
[2]. Ahmad H. &Rajagopal, K. (2013).Biological Activities of Salvadorapersica L. (Meswak).Medicinal and Aromatic Plants, 2 (4), p. 129.
[3]. Akhtar, J., Khalid, M.S., Salma, B.&Mujeeb, M. (2011).A review on phytochemical and pharmacological investigations of miswak (Salvadorapersica Linn).Journal of Pharmacy andBioallied Sciences, 3 (1),113–117.
[4]. Al-Bagieh, N.H. (1992).Antiherpes simplex virus type 1 activity of benzylisothiocyanate.Biomedical Letters, 47, 67-70.
[5]. Al-Bagieh, N.H. & Weinberg, E.D. (1988).Benzylisothiocyanate: A possible agent for controlling dental caries.Microbios Letters, 39, 143-151.
[6]. Anonymous (2001).National Horticultural Research and development Foundation. Nasik, Maharashtra, NHRDF Newsletter.
[7]. Arora1, M. & Gupta, V.K. (2011).Phytochemical and biological studies on SalvadorapersicaWall: A review.Pharmacologyonline,1, 591-601.
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| Paper Type | : | Research Paper |
| Title | : | Microbial Terrorism- A Boon to Terrrorists & Threat to Human Society |
| Country | : | India |
| Authors | : | Vivek Chauhan || Vandana Singh || Archana Tiwari |
Abstract: Microbial terrorism as the name itself signifies is the use of microbes among the certain group of people or among animals which are close to the humans or by any other means which proves to be fatal and thus causes an epidemic to occur in the areas where these Microbes have been released. As soon as the microbes are released they start showing there adverse effects like it can lead to mutation and these changes can lead to the genetic alterations which leads to death of the person once he gets effected by these microbial spores. The most well known attack of bioterrorism in the present century is that by the anthrax spores in New York in Oct.2001. It is understood that even a small bioterrorist attack can lead to the destruction in the complete economy of the country and this can prove to be the most deteriorating situation. Microorganisms act as good weapons since their mass production from a single cell is easy without much skill and infrastructure in comparison to the devastation and disease they cause.
Keywords: microbial spores, genetic alterations, anthrax, devastation, disease, bioterrorism
[1]. Budowle B, Schutzer SE, Einseln A, Kelley LC, Walsh AC, Smith JA et al. Public health. Building microbial forensics as a response to bioterrorism. Science, 2003; 301
[2]. Budowle B, Schutzer SE, Morse SA, Martinez KF, Chakraborty R, Marrone BL et al. Criteria for Validation of Methods in Microbial Forensics. Appl. Environ. Microbiol, 2008; 74(18): 5599–600.
[3]. Budowle B, Schutzer SE, Burans JP, Beecher DJ, Cebula TA, Chakraborty R et al. Quality sample collection, handling, and preservation for an effective microbial forensics program. Appl. Environ. Microbiol, 2006; 72: 6431–38
[4]. Pattnaik P, Sekhar K. Forensics for tracing microbial signatures: Biodefence perspective and preparedness for the unforeseen. Indian J of Biotechnology, 2008; 7: 23-31.
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[7]. Ussery DW, Binnewiesm TT, Oliveira G, Jarmer R, and Hallin PF. Genome update: DNA repeats in bacterial genomes. Microbiology, 2004; 150: 3519–521.
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| Paper Type | : | Research Paper |
| Title | : | Dissimilitude Response Of Peroxidases Of Dicranopteris Linearis (Burm.F.) Underw. Against Desiccation And Rehydration Stress |
| Country | : | India |
| Authors | : | Kavitha C H || Murugan K |
Abstract:Drought is one of the major environmental stress affecting water potential and turgor in the plants leading to physiological imbalance. A wide variety of lower plants are known for their desiccation tolerance during their life cycle. In the present study, desiccation tolerance mechanism of Dicranopteris linearis (Burm.f.) Underw commonly called as forking fern was analyzed in terms of ROS production (H2O2) and the varied activity of the scavenging enzyme peroxidase. Plants exposed to desiccation-rehydration stress for different periods, i. e.., 2 d to 10 d in a controlled growth chambers and the respective controls were maintained in room temperature. Desiccation induced oxidative stress was exhibited as significant rise in the level of hydrogen peroxide (H2O2) and it correlated positively with duration. Subsequently, the activity of peroxidases (POX) enzyme (both cytosolic and cell wall bound) was also examined.
[1]. Oliver, M. J., Tuba, Z., and Mishler, B. D. 2000a. The evolution of vegetative desiccation-tolerance in land plants. Plant Ecol. 151:
85–100.
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vascular plants. Plant Ecol. 151: 19–28.
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Asplenium ceterach L. Arch. Biol. Sci., Belgrade, 62 (4), 1071-1081.
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not involved in the inhibition of the auxin regulated ro 1B gene expression in tobacco leaf plants. Plant Physiology, 122 : 1379-
1385.
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| Paper Type | : | Research Paper |
| Title | : | The Antioxidant Potential of Astaxanthin on Arsenic Trioxide Induced Cardiac Damage in Male Wistar Rats |
| Country | : | India |
| Authors | : | Binu P || Mathews V Varghese || Manju Alex || Abhilash S || Vineetha R C || Harikumaran Nair R |
Abstract:Arsenic is an environmental toxic metal implicated in human diseases. Carotenoids are the most widespread group of pigments found in nature. Astaxanthin is a xanthophylls keto carotenoid with antioxidant property and commonly found in marine environment. The purpose of this study was to examine a possible cardioprotective effect of astaxanthin against arsenic induced toxicity in a rat model. Twenty four male wistar rats were randomly divided into four experimental groups, as follows: control group, arsenic trioxide treated group (4 mg/kg body weight), astaxanthin treated group (50 mg/kg body weight) and arsenic was coadministered with astaxanthin (4 mg/kg body weight + 50 mg/kg body weight) for a period of 30 days. In experimental rats, oral administration of arsenic trioxide significantly induced cardiac damage which was evident from the increased levels of serum lactate dehydrogenase (LDH), creatine kinase (CK), calcium level with a significant (p< 0.05) decrease in level of potassium. Arsenic treatment also significantly decreased the activity of non enzymatic antioxidant reduced glutathione (GSH) level in myocardium. A markedly increased level of malondialdehyde (MDA), an index of lipid peroxidation was shown in arsenic treated group.
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| Paper Type | : | Research Paper |
| Title | : | Hemolytic index – A tool to measure hemolysis in vitro |
| Country | : | India |
| Authors | : | Usha Adiga || Yogish S |
Abstract: Introduction: Hemolysis is the commonest causeof pre analytical error. Hemoglobin released interferes with analyte concentration chemically and optically. Contents of RBCs released might falsely elevate analyte concentration. This is one of the commonest cause of sample rejection which poses problem in fresh sample collection. Inconvenience caused to patients results in non co-operation for further investigations. The aim was to use HI as an automated determinant of hemolysis in venous blood specimens sent to our clinical chemistry laboratory and measure the extent of hemolysis
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| Paper Type | : | Research Paper |
| Title | : | Antimicrobial property of different parts of Citrus limon |
| Country | : | India |
| Authors | : | Sony Kumari || Firdus M. H. Siddique || Saloni D. Gupta || and Anindita Baruah |
Abstract: Lemon (Citrus) are acidic fruits of family Rutaceae. Lemon is called the tree of life because of its various medicinal properties. Almost all parts of the fruit can be used against different diseases due to its wide range of medicinal property. Other than nutritional content they also have antioxidant, antimicrobial, insect repellent, medicinal and many other properties. Different biocompoundsof the fruit can replace the function of synthetic drugs which are available in market. In the present study, the antimicrobial extent of crude juice, different extracts of leaf (matured and young), peel and standards streptomycin and tetracycline (1mg/mL) was determined against E. coli by agar diffusion method. The highest and minimum inhibition zone was found in crude juice (more than the standard) and matured leaf respectively. MIC for the crude juice was recorded as 0.01μL/mL (v/v) and for streptomycin and tetracycline was recorded as 0.1μg/mL and 0.01μg/mL respectively. The study clearly points out the antimicrobial potential emphasizing the importance of incorporating lemon as a regular component in diet.
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