Version-1 (March–April 2016)
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| Paper Type | : | Research Paper |
| Title | : | Production and Molecular Characterization of riboflavingenic Strain of Aashbya gossypii |
| Country | : | Khartoum sudan |
| Authors | : | Shami E. A. Bakhiet and Engaz Mustafa Ibrahim Mohamed |
Abstract: Riboflavin, a yellow, water soluble solid, is wide distributed in plants and animals, and plays an important role in live organisms because it is the precursor of flavin mononucleotide (riboflavin 5'-monophosphate, FMN) and flavin adenine di-nucleotide (FAD), which are functioning as coenzymes for wide variety of enzymes in intermediate metabolism. The main objective of the current study is to produce riboflavin by Ashbya gossypii, estimate the concentration of riboflavin and molecular characterization and differentiation between riboflavinogenic and non-riboflavinogenic strains....
Key words: Ashbya gossypii, Flavin adenine di-nucleotide, Riboflavin, riboflavinogenic, Spectrophotometer..
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[3] E Vandamme, Production of vitamins, coenzymes and related biochemicals and biotechnological processes. J. Chem. Technol. Biotechnol. 53 (2), 1992, 313–332.
[4] S Ashby, and W. Nowell, "The Fungi of Stigmatomycosis." Ann Bot. os-40 (1), 1926, 69–84.
[5] H Prillinger, W. Schweigkofler, M. Breitenbach, P. Briza, E. Staudacher, K. Lopandic, O. Molnar, F. Weigang, A. Ellinger, Phytopathogenic filamentous (Ashbya, Eremothecium) and dimorphic fungi (Holleya, Nematospora) with needle-shaped ascospores as new members within the Saccharomycetaceae. Yeast, Davis, 13, 1997, 945-960..
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| Paper Type | : | Research Paper |
| Title | : | Cloning, Expression and Purification of Chalcone Synthase from Solanum Tuberosum |
| Country | : | India |
| Authors | : | Pulak Das || Shuban K Rawal |
Abstract: Chalcone synthase (CHS) gene was amplified by PCR from cDNA of Solanum tuberosum leaf tissue. The gene was cloned into pGEMT easy vector and sequenced. It was then ligated into pET 30 vector to construct recombinant plasmid pET30-CHS. The recombinant was transformed into E.coli BL21 strain and the target gene was over expressed using IPTG. Optimum condition of IPTG concentration and temperature for over expression was found to be 1 mmol L-1 and 37ºC respectively. The results showed that the His-tag- CHS was highly heterogonous expressed in the inclusion bodies into the host cell. However, the His-tag- CHS fusion protein was purified using affinity chromatography with Ni2+-NTA resin column. SDS-PAGE analysis demonstrated that the host with pET30-CHS generated a 42 kD His-tag-CHS fusion protein.
Key words:Chalcone synthase; fusion protein; plasmid; cloning; purification; expression.
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[2]. T Tohge, K Yonekura-Sakakibara, R Niida, A Wantanabe-Takahasi & K Saito ,Phytochemical genomics in Arabidopsis thaliana: A case study for functional identification of flavonoid biosynthesis genes, Pure and Applied Chemistry, 79 (4) (2007) 811–23
[3]. G Hzardina & RA Jensen , Spatial organization of enzymes in plant metabolic pathways, Annul Rev Plant Physiol Plant Mol Biol, 43 (1992) 241–67.
[4]. D Saslowsky & B Winkel-Shirley, Localization of flavonoid enzymes in Arabidopsis roots, The Plant Journal: for cell and molecular biology, 27 (1) (2001) 37–48.
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| Paper Type | : | Research Paper |
| Title | : | Bcl-2(Rs956572) G/A Mutation in Cigarette Smokers and receptiveness to Lung Cancer |
| Country | : | Saudi Arabia. |
| Authors | : | Dilshad Ahmad |
Abstract: Cigarette smokingis the root cause of cancer and death from lung cancer. Singlenucleotidepolymorphisms(SNPs) are the salient location in an individual genome which influences the diseases. Since cigarette smoke contain large number of oxidants which may induce mutations. Bcl-2 SNPs have been found to be associated with predisposition of different cancers. In present study we examine the association of bcl-2 variant rs956572 with the cigarette smokers to predict the risk of smoking related cancers in smokers.....
Key words: Bcl-2, (rs956572) G, smokers, lung cancer, Single nucleotide polymorphisms
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[5]. Li A, Ojogho O, Escher A. Saving death: apoptosis for intervention in transplantation and autoimmunity.Clin. Dev. Immunol. 13 (2–4): 273–282, 2006.
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| Paper Type | : | Research Paper |
| Title | : | Purification of Glycated Hemoglobin from Hemolysate of Blood Samples and Its Application in Immunoassay. |
| Country | : | Pakistan |
| Authors | : | Mahak Fatima || Farkhanda Ghafoor || Mahjbeen Saleem |
Abstract: This study was embarked on, to produce working standards for HbA1c ELISA assay, using locally accessible materials.A pool of blood samples with high levels of HbA1c were used to make hemolysate. Estimation of Hemoglobin level and % HbA1c was done, by Bradford assay and Biorad D-10 respectively, prior to loading on Sephadex G-100 column. Peaks eluted for different hemoglobin variants were purified and confirmed by SDS PAGE. The range of working standards for in-house ELISA assay for HbA1c was made from purified fractions of HbA1c......
Key words: Diabetes mellitus,Glycated Hemoglobin, HbA1c ELISA.
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| Paper Type | : | Research Paper |
| Title | : | Microalbuminuria: An Out of Sight Risk among Non-Diabetic Hypertensive Cases Residing In A Rural Area of Lahore |
| Country | : | Pakistan |
| Authors | : | Saima Naz Mohsin || Farkhanda Ghafoor || Zunaira Safdar || Muhammad Aasim |
Abstract: Background: Microalbuminuria is an indicator of generalized vascular damage and a marker of cardiovascular complications in hypertensive. Early detection and prevention of microalbuminuria can help to control these related problems. The purpose of this pilot study was to determine urinary albumin creatinine ratio as measure of microalbuminuria in non-diabetic hypertensive patients residing in a rural area of Lahore.......
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[2]. Safdar S, Omair A, Faisal U, Hasan H. Prevalence of hypertension in a low income settlement of Karachi, Pakistan. J Pak Med Assoc. 2004 Oct; 54(10):506-509.
[3]. Bigazzi R, Bianchi S, Campese VM, Baldari G. Prevalence of microalbuminuria in a large population of patients with mild to moderate hypertension. Nephron 1992; 61: 94 – 97.
[4]. Parving HH, Jensen HE, Mogensen CE, Evrin PE. Increased urinary albumin excretion in benign essential hypertension. Lancet 1974; 1: 231 – 237.
[5]. Losito A, Fortunati F, Zampi I, Del Favero A. Impaired functional reserve and albuminuria in essential hypertension. Br Med J 1988; 1: 1562 – 1564.
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| Paper Type | : | Research Paper |
| Title | : | Correlation of Serum Bilirubin with Inflammatory Marker hsCRP in Metabolic Syndrome Disorder |
| Country | : | India |
| Authors | : | Dr. Pradipta Ghosh || Dr. Jayita Dasgupta (Ghosh) || Dr Tanima Mandal || Dr Debojyoti Bhattacharjee |
Abstract: In India the incidence and prevalence of metabolic syndrome (MS) are on the rise as suggested by various surveys [1]. Different cross sectional and longitudinal studies revealed that MS is strongly associated with inflammation [2-4]. Increased proinflammatory cytokines are seen in patients of MS. The increases in proinflammatory cytokines, including Interleukin-1 (IL-1), Interleukin-6(IL-6), Interleukin-18(IL-18), tumour necrosis factor (TNF), and C-reactive protein (CRP), gamma interferon (IFN gamma) reflect overproduction by the expanded adipose tissue mass.......
Key words: Metabolic syndrome, Bilirubin, hs-CRP, Antioxidant
[1]. Grundy SM.Metabolic syndrome pandemic . Arterioscler Thromb VascBiol 2008;28:629-36
[2]. Rutter MK, Meigs JB, Sullivan LM, D'Agostino RB, Sr., Wilson PW. C-reactive protein, the metabolic syndrome, and prediction of cardiovascular events in the Framingham Offspring Study. Circulation. 2004 Jul 27;110(4):380-5.
[3]. Wang YY ,Lin SY , Liu PH ,Cheung BM, Laiwa. Association between hematological parameters and metabolic syndrome components in a Chinese population.J.Diabetes Its Complicat 2004; 18:322-7
[4]. Festa A, D'Agostino Jr R, Howard G ,Myk Kanen L, Tracy RP, Haffner SM. Chronic subclinical inflammation as part of the insulin resistance syndrome :the Insulin Resistance Atherosclerosis Study (IRAS). Circulation 2000; 102:42-7
[5]. Mark B, Pepys and Gideon M, Hirsachfield. C-reactive protein; a critical update 2003; J Clin Invest. 111(12); 1805-1812.
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| Paper Type | : | Research Paper |
| Title | : | Evaluation of Ischemia Modified Albumin in Hyperlipidemia Patients |
| Country | : | India |
| Authors | : | Dr. Margit Gajjar || Dr.H.B.Sirajwala |
Abstract: Introduction: Lipid disorders are mainly related to abnormalities in metabolism of lipoproteins. Hypercholesterolemia is associated with oxidative stress, resulting into atheromatous plaque development and it reduces the capacity of albumin to bind cobalt owing to ischemia, resulting in an increased IMA (Ischemia Modified Albumin). IMA formation appears to be associated with oxidative stress and ischemia.The present study is undertaken to evaluate level of IMA in patients suffering from hyperlipidemia.......
Key words: Ischemia modified albumin, Oxidative Stress, Atheromatous plaque.
[1]. Emmanuelle Mothes, Peter Feller, The albumin cobalt binding test for detecting myocardial ischemia;France biochemistry,2007
[2]. Enas A.,Clinical implications:Dyslipidemia in the Asian Indian population;American association of physicians of Indian origin, 2002.
[3]. Elena Citkowitz, William L Ishley, Hypercholesterolemia, Polygenic study;2009.
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[5]. Louis P, Florence M,Christine B.Hypercholesterolemia increases endothelial dysfunction, lipid content and accelerated atherosclerosis;Journal of Atherosclerosis and Thrombosis, Vol 20,2000.
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| Paper Type | : | Research Paper |
| Title | : | An Insight into Simulated Product Development: Hibiscus Tea |
| Country | : | India |
| Authors | : | Dr. Jyoti D. Vora || Aditi U. Patwardhan || Padma Srinivasan |
Abstract: Simulated product development is the new innovation in the field of Food Science and Food technology. It is an armchair science that accurately estimates the pulse of consumer preferences. The product is developed in reference to an imaginary framework based on fixed attributes of the proposed product. The simulated attributes are usually product attributes, which are of prime importance to both the manufacturer and the consumer........
Key words: Hibiscus, Hibiscus tea, novel product development, simulated product development.
[1] SerbanC ,Sahebkar A, Ursoniu S, Andrica F, Banach M.; Effect of sour tea (Hibiscus sabdariffa L.) on arterial hypertension: a systematic review and meta-analysis of randomized controlled trials. Journal of Hypertension.2015 Jun;33(6):1119-27. doi: 10.1097/HJH.0000000000000585.
[2] Hopkins AL, Lamm MG, Funk JL, Ritenbaugh C.; Hibiscus sabdariffa in the treatment of hypertension and hyperlipidemia: a comprehensive review of animal and human studies. Fitoterapia. 2013 Mar;85:84-94. doi: 10.1016/j.fitote.2013.01.003. Epub 2013 Jan 17.
[3] Lin HH, Chen JH, Wang CJ.; Chemopreventive properties and molecular mechanisms of the bioactive compounds in Hibiscus sabdariffaLinne. Current Medicinal Chemistry. 2011;18(8):1245-54.
[4] Nwachukwu DC, Aneke E, Nwachukwu NZ, Obika L, Nwagha UI, Eze AA.; Effect of Hibiscus sabdariffaon blood pressure and electrolyte profile of mild to moderate hypertensive Nigerians: A comparative study with hydrochlorothiazide. Nigerian Journal of Clinical Practice. 2015 Nov-Dec;18(6):762-70. doi: 10.4103/1119-3077.163278.