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| Paper Type | : | Research Paper |
| Title | : | Studies on Protein Composition of Pigeon Pea [Cajanus Cajan (L.) Millspaugh] Treated With Sodium Azide and Gamma Radiation |
| Country | : | Nigeria |
| Authors | : | Mathew BA || Sule HA || Toluhi OJ || dachaba SO || Ibrahim AA || Abuh SJ |
Abstract: The aim of this study was to determine the protein composition of pigeon pea Cajnus cajan (L) millspaugh treated with sodium azide and gamma radiation. This was performed by exposing the seeds of landraces pigeon pea to gamma rays at Centre for Radiotherapy and Oncology Department, ABUTH, Shika, Zaria at doses of 0(control), 50, 100, 150 and 200Gy. These seeds were further treated with sodium azide (NaN3) concentrations at 0.00, 0.01, 0.02, 0.03 and 0.04% SA. The analysis of protein content was the determination of the amount of reduced Nitrogen present in food substance, i.e., its –NH2 and =NH2. The proximate analyses were carried out as recommended by the Association of Official Analytical Chemists (AOAC, 1990) using the kjeldahl method. The highest crude protein (CP) percentage value was obtained from 150Gy + 0.03%SA (25.1 %), followed by that due to 100Gy + 0.01%SA (24.7 %), 100Gy + 0.04%SA (24.4 %), 100Gy + 0.03%SA (23.7 %) and 150Gy + 0.00%SA (23.6 %). It is, therefore, concluded that the two mutagens significantly affected the pigeon pea plant composition.
Keywords: Protein composition, crude protein, nitrogen, sodium azide, gamma radiation, pigeon pea, Cajanus cajan.
[1]. A.O.A.C (1990). Association of Official Analytical Chemists. Official Methods of Analysis, 15th Edition., Washington, DC.800 – 950
[2]. Center for New Crops and Plant Products (2002). Cajanus Cajan (L.) Millsp. Purdue University. Http://Www.Hortculture.Purdue.Edu/Newcrop/DukeEnergy/Cajanus-Cajan.Html. P: 6.
[3]. Edwards, D.G. (198I). Development of Research on Pigeon Pea Nutrition, In: Nene, SH., Hall, and Sheila, V.K. The Pigeon Pea. CAB International, Wellingford, UK and New York. P212.
[4]. John, H. (2005). Principles of Food Crop Production. Journal of Food Science, 4: 41 – 47.
[5]. Khan, S., Mujeeb, U. R., Meheraj-Ud-Din, B. and Bahar A. S. (2000). MMS induced biological damage and polygenic variability in greengram [Vigna radiata (L.) Wilczek]; LegumeResearch, 23(2): 126-129.
[6]. Okaka, J.C., Akobundu, E.N.T. and Okaka, A.N. (2002). Human Nutrition: An Integrated Approach. 2ndEdition, OCJANCO Academic Publishers, Enugu, Nigeria, Pp: 312-320.
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| Paper Type | : | Research Paper |
| Title | : | Isolation of Alkaloid from a Medical Plant (A Case Study of Morinda Lucida) |
| Country | : | Nigeria |
| Authors | : | Akpagu Francis C. || Nnamani Emmanuel V. || Eze Chukwuebuka G. I. |
Abstract: The isolation and detection of alkaloids content of Morinda lucida (Ezeogwu) from Rubiceace family, a medicinal plant was carried out using solvent extraction process. The dried powdered leaves of the plant were divided into batches. Different solvents were used on them. After 6 days of occasional shaking, it was filtered. The filtrates were used for testing the presence of alkaloids in Morida Lucida. Mayer's reagents Wagner and Lugol's reagents and 5m sodium hydroxide were used as detecting reagents. Mayer's reagent yields cream precipitate in both acidic and alkaline extracts. Wagner and Lugol's reagents yield reddish brown precipitate in both acidic and alkaline extracts. 5ml sodium hydroxide gave white swirling precipitate. Other coloured precipitate like orange and pale orange was gotten as a result of difference in solvents used for isolation. The presence of the above precipitate indicates the presence of alkaloids in Morinda Lucida.
Keyword: Morinda lucida, Rubiceace, alkaloids, isolation and detection.
[1]. Awasthi, A. and Aepita, V. (2007): '' Phenyal Kyalamines as Calcium Channels Blockers''. Journal of chemistry 199(5): 565-570.
[2]. Bentley, K. W (1999): '' The Chemistry of Natural Products'' . 5th ed. Interscience Fattorusso, E, Tag Lialatela, S and Orazio, V(2008): '' Modern Alkaloids Structure, Isolation, Synthesis and Biology''. 2nd ed., Wiley VCH:USA, 81-431
[3]. Finar, I.L. (2007). ''Stereochemistry and Natural Product" . 5th ed. Thearson Education: India, 710-782.
[4]. George, R.K. and Kelvine, M.C. (2009). '' Isolation of alkaloids from plant''. Plenum Press, New York, 52-101
[5]. Hesse, M. (2005): '' Alkaloid Nature's Curse or Blessing'' , 1st ed. Plenum Press: New York, 11-14
[6]. Holland, M.; Heber, L.; Jian-Xian, G.; Francisco, O.; Mercedes, C., Paula, F. and Andrew, J.W. (1999): Enanto Selective Synthesis and Pharmacological Evaluation''. Journal of Pharmaceutical Research, 16, 281-287.
[7]. Keay, R.W. ; Onochie, A. and Stanfield, D.P (1998). '' Useful Plants of West Africa'', Federal Department of Forest Research: Ibadan, 27-30.
[8]. Kuznestsova, O.F. ; Mosevich, I.K.; Korsakov, M. C.; Fedorova, O.S and Krasokowa, R.N. (2002): ''Asymmetric Synthesis of Dihydropheny Alamine'' Radiochemistry, 44 (6): 582-587.
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| Paper Type | : | Research Paper |
| Title | : | Estimation of Protein Content in Soyabeans Consumed In Abakaliki, Ebonyi State, Nigeria |
| Country | : | Nigeria |
| Authors | : | Akpagu Francis C. || Nnamani Emmanuel V. || Eze Chukwuebuka G. I. |
Abstract: The percentage composition of protein, ash content and moisture present in soyabeans consumed in Abakaliki, Ebonyi state were studied. The result showed that sample A which is cultivated at International Institute for Rural Development (IIRD) Igbeagu, Izzi Local Government Area has the highest Percentage protein content of 39.23 with ash content of 5.30% and moisture content of 8.20% sample B which were bought from Benue State has a Percentage protein content of 37.68, ash content of 5.00% and moisture content of 10.33%. while sample C which were bought from Taraba State has the lowest protein content of 36.74%, ash content of 4.57% and moisture content of 10.97% all the soyabeans sample studied meets the protein standard value of between 36.50 -40 percent.
Keywords: Protein, ash content, soyabeans, Abakaliki and Ebonyi state.
[1]. Mayhew, S and Penny, A. (1988). Tropical and Subtropical Foods, 2nd Edition, Academic press, London. Pp. 81-84.
[2]. Reynold, M.W. (1962). Technology and Culture. Henry Ford's Science and Technology for Rural America. John Hopkins University Press. Vol 3, No.3 Pp 247-253
[3]. Derbyshire, E.; Wright, D.J and Boulter, D. (1976). Legumin and Vicilin, Storage Proteins and Legume seeds. Journal of Phytochemistry (Elsevier Science Ltd).
[4]. Food and Agriculture Organization of the United Nations (2007). Soybean Countries by Commodity 2007. http//faostat.fao.org/site/339/default.aspx.9/8/2010.
[5]. Raj, P.(2008). Stuffed and Starved from Farm to Fork, the Hidden Battle for the World Food System. Portobello Books Ltd, London PP. 169-173.
[6]. Thomas, R.J.M and Deman, L. (1989). Soymilk and Tofu Properties as Influenced by Soybeans Storage Conditions. American Journal of Oil Chemistry. 66: 772-782.
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| Paper Type | : | Research Paper |
| Title | : | Cystic Diseases of the Organs |
| Country | : | India |
| Authors | : | Sathialakshmi V. || Swayam Jothi S. || Saroja Sundararajulu. || Kafeel Hussain A., Sree Lekha D. || Rajeshwar Rao || N. Sssmc & Ri & |
Abstract:The cystic diseases in various organs were observed in the anatomy and OBG depaartment, in that the polycystic disease of ovary was very common compared to all other organs cystic diseases
[1]. Bae KT, Zhu F, Guay-Woodford LM, et al. Magnetic resonance imaging evaluation of hepatic cysts in early autosomal dominant polycystic kidney disease. Clin J Am Soc Nephrol 2006; 1: 64–69.
[2]. BBaaee KKTT,, ZZhhuu FF,, CChhaappmmaann AABB,, TToorrrreess VVEE,, GGrraanntthhaamm JJJJ,, GGuuaayy--Woodford LM, Baumgarten DA, King BF, Wetzel LH, Kenney PJ, Brummer ME, Bennet WM, Klahr S, Meyers CM, Zhang X, Thompson PA, Miller JP. Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP); Magnetic resonance imaging evaluation of hepatic cysts in early autosomal dominant polycystic kidney disease: the Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease Cohort. Clin J Am Soc Nephrol. 2006; 1; 64-9. (PubMed: 17699192)
[3]. BBiisscceeggiillaa eett aall RReennaall CCyyssttiicc DDiisseeaasseess :: A Review ,Advances in Anatomic pathology Jan 2006-vol 13 ,Issue 1 pp26-56
[4]. Balen A, Michaelmore K. What is polycystic ovary syndrome? are national views important? Hum Product 2002; 17(9); 2219-2227.
[5]. Baert L.(1978) Kidney Int. 13, 519-525.
[6]. Davila S, Furu L, Gharavi AG, et al. Mutations in SEC63 cause autosomal dominant polycystic liver disease. Nat Genet 2004; 36: 575–77.
[7]. Drenth JP, Te Morsche RH, Smink R, Bonifacino JS, Jansen JB. Germline mutations in PRKCSH are associated with autosomal dominant polycystic liver disease. Nat Genet 2003; 10: 10.
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| Paper Type | : | Research Paper |
| Title | : | Alcoholic liver cirrhosis & herbs |
| Country | : | India |
| Authors | : | Dr Sudhir Kr Sinha |
Abstract:Alcohol is a toxin in higher doses, when it is associated with polyunsaturated fatty acids (PUFA) induces oxidative stress & hepatotoxicity Alcohol destroys the cells lining in the stomach and intestines that mediate the absorption of nutrients. Alcohol greatly increases lead absorption. Low concentration of lead may disturb normal biochemical process in the hepato billary system. Lead overload causes liver cells to become enlarged into hyperplasia (initiating the formation of tumours in the liver); Liver cells die more rapidly and are replaced by fatty deposits. Acetaldehyde, a major toxic metabolite, is one of the principal culprits mediating fibrogenic and mutagenic effects of alcohol in the liver. Liver cirrhosis leads to depletion of nutrients The by-products of alcohol metabolism generate oxidants that can contribute to cell damage. An imbalance between oxidants and antioxidants (substances that neutralize oxidation) can create oxidative stress, a state marked by continued production of oxidizing agents and escalation of cell damage,
[1]. "Alcoholic Liver Disease: Introduction". Johns Hopkins Medicine: Gastroenterology & Hepatology. Baltimore, MD: Johns Hopkins Hospital. 2010. Retrieved 27 January 2010. [2]. "Alcoholic Liver Disease". Common Gastrointestinal Problems: A Consumer Health Guide. Arlington, VA: American College of Gastroenterology. 2010. Retrieved 27 January2010. (Plain text version also available.)
[3]. Borowsky SA, Strome S, Lott E. Continued heavy drinking and survival in alcoholic cirrhotics. Gastroenterology 1981; 80:1405–1409.
[4]. Luca A, Garcia-Pagan JC, Bosch J, Feu F, Caballeria J, Groszmann RJ, et al. Effects of ethanol consumption on hepatic hemodynamics in patients with alcoholic cirrhosis. Gastroenterology 1997; 112: 1284–1289. [5]. Bjelakovic G, Gluud LL, Nikolova D, Bjelakovic M, Nagorni A, Gluud C (2011). Bjelakovic, Goran, ed. "Antioxidant supplements for liver diseases". Cochrane Database Syst Rev(3): CD007749. doi:10.1002/14651858.CD007749.pub2. PMID 21412909. [6]. Ferenci P, Dragosics B, Dittrich H, Frank H, Benda L, Lochs H, et al. (1989). "Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver". J Hepatol 9 (9): 105–113. doi:10.1016/0168-8278(89)90083-4.
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| Paper Type | : | Research Paper |
| Title | : | UV Mutagenesis Enhanced Biotransformation Efficiency of Rutin to Isoquercitrin by Bacillus litoralis |
| Country | : | India |
| Authors | : | Qiuyue Wang, Ye Li || Jing Wang, Zhitang Lu |
Abstract: In order to obtain high biotransformation efficiency of rutin to isoquercitrin (quercetin-3-O-glucoside), Bacillus litoralis C44 was treated by UV mutagenesis to screen the thermo- and alkali-tolerant mutants, for these conditions allow for a very high substrate concentration. The optimal mutagen dose for strain C44 was irradiation for 50s with a 15W UV lamp from 30 cm away. The mutants were preliminary screened by quantitative TLC, and 16 mutant strains were faced to second-screening by HPLC. As a result, a genetic stable mutant strain UV-2-45 was obtained, which got a biotransformation rate of 3.9 times more than the original strain Bacillus litoralis C44, and its mole yield reached as high as 91% from 3 g/L of rutin in glycine-sodium hydroxide buffer (pH 9.0) at 45°C for 2 days.
Keywords: Isoquercitrin, UV Mutagenesis, Bacillus litoralis
[1]. K. Valentová, J. Vrba, M. Bancířová, Isoquercitrin: Pharmacology, toxicology, and metabolism, Food and Chemical Toxicology, 68, 2014, 267-282. [2]. S. H. Jung, B. J. Kim, E. H. Lee, et al. Isoquercitrin is the most effective antioxidant in the plant Thuja orientalis and able to counteract oxidative-induced damage to a transformed cell line (RGC-5 cells), Neurochemistry international,57(7),2010, 713-721.
[3]. R. Zhang, Y. Yao, Y. P. Wang, Antidiabetic activity of isoquercetin in diabetic KK-Ay mice, Nutrition & Metabolism, 8(85), 2011,2-6.
[4]. Y. Kim, S. Narayanan, K.O. Chang, Inhibition of influenza virus replication by plant-derived isoquercetin, Antiviral Research, 88(2), 2010, 227-235.
[5]. A. Gasparotto Junior, F. M. Gasparotto, M. A. Boffo, Diuretic and potassium-sparing effect of isoquercitrin—An active flavonoid of Tropaeolum majus L, Journal of ethnopharmacology, 134(2), 2011, 210-215.
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| Paper Type | : | Research Paper |
| Title | : | DNA damage repair Neil3 gene Knockout in MOLT-4 |
| Country | : | Iraq |
| Authors | : | Perry H. Saifullah |
Abstract: RNAi is superannuated cellular mechanism that protect organism against viruses that replicate through double- stranded RNA. RNAi can be used to diminish gene expression from plasmid expressing and inserted sequence repeat. A stable harpin would be expressed after the vector was integrated into the genome. In this paper a shiRNA expressing vector for Neil3 was designed and developed which is capable of replication in MOLT-4. This shiRNA vector had the ability to arose the RNAi pathway, and reduce the gene expression of Neil3. This was assessed by using pSilence 4.1CMV as a vector, and Gapdh as positive control.
Key words: Neil3, Gapdh, shiRNA, MOLT-4, gene knockout
[1]. Kim YJ1, Wilson DM 3rd. Overview of base excision repair biochemistry, Curr Mol Pharmacol. 2012 Jan;5(1):3-13
[2]. Yngve Sejersteda,b,c,1, Gunn A. Hildrestranda,c,1, David Kunkea,c,2, Veslemøy Rolsetha,c, Silje Z. Krokeidea,et.al Endonuclease VIII-like 3 (Neil3) DNA glycosylase promotes neurogenesis induced by hypoxia-ischemia, 18802–18807 | PNAS | November 15, 2011 | vol. 108 | no. 46.
[3]. Jia Zhou1, Minmin Liu1,3, Aaron M. Fleming2, Cynthia J. Burrows2, Susan S. Wallace, Neil3 and NEIL1 DNA glycosylases remove oxidative damages from quadruplex DNA and exhibit preferences for lesions in the telomeric sequence context( 2013), Journal of Biological Chemistry, 288, 27263-27272.
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| Paper Type | : | Research Paper |
| Title | : | Ropivacaine A Review of Its Use In Regional Anaesthesia, Choric Pain Management And In Patients With Cardiac Diseases In Non Cardiac Surgeries |
| Country | : | India |
| Authors | : | Dr. Vasudha Jadhav || Dr. Ranjeetsinh Jadhav || Dr. B. M. Diwanmal, |
Abstract: Ropivacaine is a long acting amide local anesthetic agent ,it is the pure S(-)- enantiomer of propivacaine , and is a long acting amide local anaesthetic agent , eliciting nerve block via reversible inhibition of sodium on influx in nerve fibres. Ropivacaine is less lipophilic than bupivacaine and is less likely to penetrate large mylinated motor fibres, resulting in a relatively reduced motor blockade .The reduced lipophilicity is also associated with decreased potential for central nervous system toxicity and cardiotoxicity.
[1]. Hansen TG. Ropivacaine: A pharmacological review. Expert Rev Neurother 2004 ;781-91.
[2]. Kindler CH , Paul M, Zou H, Liu C , Winegar BD, Gray AT ,et al.Amide local anesthetics potently inhibit the human tandem pore domain background K+ channel TASK -2 (KCNK5). J Pharmacol EXP Ther 2003;306:84-92.
[3]. Graf BM, Abraham I, Eberbach N, Kunust G, Stowe DF ,Martin E. Differences in cardiotoxicity of bupivacaine and ropivacaine are the result of physicochemical and stereoselective properties . Anaesthesiology 2002 ;96:1427-34.
[4]. Graf BM. The cardiotoxicity of local anaesthetics : The place of ropivacaine . Curr Top Med Chem 2001;1:207-14.
[5]. Cederholm I, Evers H, Lofstrom JB. Skin blood flow after intradermal injection of ropivacaine in various concentrations with and without epinephrine evaluated by laser Doppler flowmetry. Reg Anesth 1992 ;17:322-8.
[6]. Porter J. Crowe B , Cahill M, Shorten G. The effects of ropivacaine hydrochloride on platelet function: An assessment using the platelet function analyser (PFA-100). Anaesthesia 2001;56:15-8.
[7]. Batai I, Kerenyi, Falvi J, Szabo G . Bacterial growth in ropivacaine hydrochloride .Anaesth Analg 2002;94:729-31.
[8]. Kampe S, Potter C, Buzello S, Wenchel HM, Paul M, Kiencke P, etal .Ropivacaine 0.1% with sufentanil 1microg/mL inhibits in vitro growth of Pseudomonas aeruginosa and does not promote multiplication of staphylococcus aureus . Anesth Analg 2003;97:409-11.
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| Paper Type | : | Research Paper |
| Title | : | Influence Of Different Nitrogen And Organic Carbon Sources On Microalgae Growth And Lipid Production |
| Country | : | India |
| Authors | : | Amit Kumar Sharma || Pradeepta Kumar Sahoo || Shailey Singhal |
Abstract: Microalgae based biofuels are getting attention due to energy crisis and enviromental protection. In the present study, the Chlorella sp. was cultivated in BG-11 medium at batch mode. The effect of different nitrogen (sodium nitrate, potassium nitrate and urea) and organic carbon (glucose, glycerol and sucrose) sources were analyzed on growth and lipid accumulation on this species. The highest biomass growth and biomass productivity of chlorella sp. was found 1.29±0.04 g/l, 76.96±4.5mgl-1d-1 in urea. However in case of organic sources, the biomass growth and productivity was found maximum in glucose (1.43±0.075 g/l 86.04±3.2 mgl-1d-1). The lipid content was examined using folch method and found better in potassium nitrate nitrogen source (11.84%) . Among organic carbon sources, the maximum lipid content (13.22% and lipid yield 189.94 mg/l were found in case of glucose, followed by glycerol and sucrose. Various properties of biodiesel obtained from chlorella sp. such as Cetane number, Saponification value, Iodine value and Degree of unsaturation were followed standerds set by the national petroleum agency (ANP255), ASTMD6751 and EN14214. Keywords: Biodiesel, Biomass growth, Chlorella sp., Lipid extraction, Transesterification
[1]. I.B. Muhit, D. Baidya, Nurangir Nahid, Prospect of Algal Biodiesel Production in Bangladesh: Overview from Developed Countries, IOSR-JMCE, 11(1), 2014, 49-54.
[2]. Antoni. D.; Zverlov, V.V.: Schwarz, H. Biofuels from Microbes. Appl. Microbiol. Biotechnol. 2007, 77, 23-35.
[3]. BP Statistical Review of the world energy; June 2008.
[4]. Abdelaziz, A.E., Leite, G.B., Hallenbeck, P.C.,. Addressing the challenges for sustainable production of algal biofuels: I. Algal strains and nutrient supply. Environ. Technol. 34, 2013a, 1783–1805.
[5]. Amaro, H.M., Guedes, A., Malcata, F.X.,. Advances and perspectives in using microalgae to produce biodiesel. Applied energy, 88, 2011, 3402–3410.
[6]. Leite, G.B., M.Abdelaziz, A.E., Hallenbeck, P.C.,. Algal biofuels; challenges and opportunities. Bioresource Technology. 145, 2013, 134–141.
[7]. Chisti, Y., 2007. Biodiesel from microalgae. Biotechnol. Adv. 25, 2007, 294–306
[8]. Yoo C, Jun SY, Lee JY, Ahn CY, Oh HM () Selection of microalgae for lipid production under high levels carbon dioxide. Bioresour Technol 101, 2010, 71–74
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| Paper Type | : | Research Paper |
| Title | : | D-Value Determination Of Environmental Isolates From Sterile Manufacturing |
| Country | : | Pakistan |
| Authors | : | W.Jamil1, A. Zahid || A.Jabbar || Z.Ali || S. Faraz Ikram |
Abstract: Aqueous spore suspensions of 3 different species of Bacillus isolated from Sterile manufacturing were heated at different temperatures for various time intervals in a water bath, counted by plate count method on Tryptic Soy Agar (TSA), and incubated at 32.5 °C. Remaining or resistant spore of isolated Bacillus species form sterile manufacturing of form colonies on TSA. D-Value, the decimal reduction time (time required at a given temperature for destruction of 90% of the cells), when survivors were colonized on TSA and the calculated D-Value are for SMF-B-001, 13.19 ± 0.05919, for SMF-B-002, 12.25 ± 0.051919 and SMF-B-003, 14.09 ± 0.05319 respectively. Recovery on TSA resulted in reduced decimal reduction time showing that damage occurred early in the thermal dealing. Heat sensitive spores showed sensitivity also to other post processing environmental factors such as incubation temperatures, a pH of 7.3 ± 0.3 for the medium.
Keywords: Spore= Most resistant form of vegetative cells ,TSA= Tryptic Soy Agar, Incubation= specicific temperature or condition which helps microorganism to grow, SMF= sterile manufacturing facility.
[1] P. Thereza Christina Vessoni, Irene A. Machoshvili, Maria Elena Santos Taqueda, and Carlos Augusto Montenegro Ferraz. Bacillus Stearothermophilus Sporulation Response to Different Composition of Media to evaluate the effectiveness of 11 commonly used ingredients to improve Bacillus stearothermophilus ATCC 7953 sporulation. PDA J Pharm Sci Technol 52 1998.198-208.
[2] I I.R, A.E Oji, O.K Nwosu, E.S Amadi. Antimicrobial activiy salvon, Izal and Z-germicide Against clinical isolates of peseudomona aeruginosa from hospitals wards.European Jouranl of dentistry and medicine 3 (1) 2011, 32-35.
[3] O.OA, AB Olayemi, KIT Eniola, OA Adeyeba. Anti bacterial activity of some selected disinfectant regualrly used in hospitals.African Journal of Clinical and Experimental Microbiology 5(1) 2004, 126-130.
[4] B.Maria Lucia Morcerf, Vania Lucia Silva1, Felipe Augusto Sa Moreira1, Girlene Alves Silva2 and Claudio Galuppo Diniz. Antimicrobial resistance and disinfectants susceptibility of persistent bacteria in a tertiary care hospital.Journal of Microbiology and Antimicrobials Vol. 2(8), 2010, 105-112.
[5] G.Masood, Zahra Azimi. Isolation Identification and Antimicrobial Susceptibility of Pseudomonas spp. Isolated from Hospital Environment in Tonekabon, North of Iran Journal of Applied & Environmental Microbiology,2 (4), 2014, 97-101.
[6] A.Ranya Aly, Safinaz Aly Mady, Hoda H. Yusef, and Soraya Sabry. Determination of decimal reduction time (D-value) of chemical agents used in hospitals for killing airborne isolated bacteria Due to the growing numbers of infection outbreaks in hospitals. African Journal of Microbiology Research Vol. 7(26), 2013 , 3321-3330.
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| Paper Type | : | Research Paper |
| Title | : | Ethnopharmacological survey of Layyah District Punjab, Pakistan |
| Country | : | Pakistan |
| Authors | : | Akhtar J || C.M. Ayyub || K.Y. Abbasi || I.Qadir Bhabha || S. Kamal || A. Manan. |
Abstract: Ethnobotanical survey was conducted district Layyah, during 2010. The survey was conducted along with Chowk Azam road in some of villages including chaks i.e chak no 145.A/T.D.A,146.A/T.D.A,151/T.D.A,128/T.D.A and chak no 120/T.D.A tehsil and district Layyah. From each village 20 People were selected randomly by lottery method for consultation. For more ethnobotanic information some traditional healers were interviewed. Present study focused on local inhabitants who use traditional resources for self-medication with particular reference to ethnopharmacological application of plant species for diseases and natural cosmetics.
[1]. Abbasi, A.M., G. Dastagir, F. Hussain, P. Sanaullah. 2005. Ethnobotany and marketing of crude drug plants in district Haripur, Pakistan. Pakistan Journal of Plant Sciences 11, 103–114.
[2]. Abbasi, A.M., M.A. Khana, M. Ahmad, M. Zafar, S. Jahan and S.Sultanaa.2010.Ethnopharmacological application of medicinal plants to cure skin diseases and in sfolk cosmetics among the tribal communities of North-West Frontier Province, Pakistan. Journal of Ethnopharmacology 128, 322–335.
[3]. Abbasi, A.M., M.A. Khan, M. Ahmad, M. Zafar, H. Khan, N. Muhammad, S. Sultana. 2009. Medicinal plants used for the treatment of jaundice and hepatitis based on socio-economics documentation. African Journal of Biotechnology 8,1643–1650.
[4]. Abdel-Hassan, I.A., J.A. Abdel-Barry, Tariq Mohammeda, S. 2000. The hypoglycemic and antihyperglycemic effect of Citrullus colocynthis fruit aqueous extract in normal and alloxan diabetic rabbits. Journal of Ethnopharmacology 71 (1_/2), 325_/330.
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| Paper Type | : | Research Paper |
| Title | : | Study of Ligand Based Virtual Screening Tools in Computer Aided Drug Designing For Oral Cancer |
| Country | : | India |
| Authors | : | Vijay Laxmi Saxena1 || Shrasti Gupta || Shrashti Srivastava |
Abstract: Insilico potential drug target identification involves chemical compounds for inhibiting or promoting chemical reactions in living organism computational methods are needed for screening through large database of these to identify a inhibitor for receptor 0ral Multi-AGC Kinase was employed for docking.We investigated the detailed pharmacology and antitumour activity of the novel clinical drugs and natural ligand. It is associated with Oral Multi-AGC kinase protein. The various ZINC analogs on basis of 99%, 95%, 90% similarity for best docking results with protein for predicting a ZINC analog which can be identified under the category of becoming a potential drug candidate in future. The best mol dock score were for ZINC analog ZINC ID 72131268 for Irinotecan is -169.087 kcl/mol, ZINC ID 04166028 for Teniposide is -178.235 kcl/mol, ZINC ID 30731084 for Topotecan is -166.939 kcl/mol and ZINC ID 00899824 for Curcumin is -141.537 kcl/mol. The two parameter for toxicity properties i.e. Oral LD50 and Mutagenicity were calculated. Oral LD50 and Mutagenicity values are found.
Keywords: Computational methods screening, Database, Insilico, Drug target, Inhibitor, Receptor.
[1]. Brad W.Neville, DDS;TerryA.Day,MD,FACS.Oral Cancer and Precancerous Lesions.CACancer J Clin 2002;52;195-215
[2]. Courtney KD, Corcoran RB, Engelman JA. The PI3K pathway as drug target in human cancer. J ClinOncol 2010;28:1075–83.
[3]. Chandarlapaty S, Sawai A, Scaltriti M, Rodrik-Outmezguine V, Grbovic-Huezo O, SerraV, et al. AKT inhibition relieves feedback suppression of receptor tyrosine kinase expression and activity. Cancer Cell2011;19:58–71.
[4]. Engelman JA. Targeting PI3K signalling in cancer: opportunities,challenges and limitations. Nat Rev Cancer 2009;9:550–62.
[5]. FaliS.Mehta, M.B.Gupta, J.J.Pindborg,R.B. Bhonsle,P.N. Jalnawalla,&P.N. Sinor. An intervention study of oral cancer and precancer in rural Indian population:a preliminary report .Bullenten of the World Healtj organization 60(3):1982.
[6]. Fan QW, Knight ZA, Goldenberg DD, Yu W, Mostov KE, Stokoe D, et al.A dual PI3 ,kinase/mTOR inhibitor reveals emergent efficacy in glioma.Cancer Cell 2006;9:341–9.
[7]. John Doorbar. The Papillomavirus life cycle. Doi:10.1016/j.jcv.2004.12006.
[8]. Liu P, Cheng H, Roberts TM, Zhao JJ. Targeting the phosphoinositide 3-kinase pathway in cancer. Nat Rev Drug Discov 2009;8:627–44.
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| Paper Type | : | Research Paper |
| Title | : | Microbiological Investigations of Selected Flies of Public Health Importance from a Waste Dump Site in Port Harcourt, Nigeria |
| Country | : | Nigeria |
| Authors | : | Ogbalu, O. K. || Douglas, S. |
Abstract: Abstract:Bacteria associated with flies of public health importance in Nigeria are not well known and their ecology is also not well understood. We aim to determine the bacteria associated with flies of waste dump site. Three flies of public health significance were collected from a waste dump site of the Rivers State University of Science and Technology, Port Harcourt. The three dipterous flies were Luciliasericata, Chrysomyasp and Musca domestica..The three flies were all of medical importance.The microbial load on three species of flies was investigated using standard plate count methods.
[1]. Ogbalu, O. K., Orlu, E. E., Eze, C. N. and Wokem, G. [2014]. Factors Associated with the Prevalence of Vulvar Myiasis Infection in Rural Nursing Mothers of the Niger Delta, Nigeria IOSR Journal of Pharmacy and Biological Sciences. Vol. 9, Issue3:81-87.
[2]. Ogbalu, O.K., Achufusi, T. G. O., Orlu, E. E. [ 2013]. Epidemiology of human furuncularmyiasisofCordylobiaanthropophagaGrunberg] in Nigeria. International Journal of Dermatology, Vol. 52 Issue 3: 331-336.
[3]. Zurek, L and Ghosh, A. [2014].Insects represent a Link Between Food Animal Farms and the Urban Environment for Antibiotic-resistant Traits Cross Reference].
[4]. CDC [2013]. Antibiotic Resistant Threats in the United States, 2013.A Cross reference.
[5]. Zurek, L. [2013]. HousefliesMay Carry Antibiotic-Resistant Bacteria Found in Wastes
[6]. Ogbalu,O. K. and Adebanji, A.[2014].The distribution of Hermetiaillucens in Oil Palm Piths of the Niger Delta. IOSR JAVS [In Press].
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| Paper Type | : | Research Paper |
| Title | : | Medium Composition Effects on Growth Kinetic of Cordyceps militaris Cells Using Agar Plate Method |
| Country | : | Egypt |
| Authors | : | Mohammad Soltani || Mohanad Al-Ali || Nor Zalina Othman || Roslinda Malik || Nagib Elmarzugi || Ramlan Aziz || Hesham A. El-Enshasy |
Abstract:Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process.
[1]. Y Jiang, and YJ Yao, Current understanding of molecular systematics of Cordyceps. Journal of Fungal Research, 2, 2004, 58-67.
[2]. H El Enshasy, and R Hatti-Kaul, Mushroom Immunomodulators: unique molecules with unlimited applications. Trends in Biotechnology, 31, 2013, 668-677.
[3]. GH Sung, NL Hywel-Jones, JM Sung, JJ Luangsa-ard, B Shrestha, JW Spatafora, Phylogenetic classification of Cordyceps and the Clavicipitaceous fungi. Studies in Mycology, 57, 2007, 5-59. [4]. MS Torres, and JF White, Clavicipitaceae: Free-Living and Saprotrophs to Plant Endophytes, in M. Schechter (ed.), Encyclopedia of Microbiology, Vol. 1, 3rd. Ed. (Oxford, Academic Press, 2009) 422-430.
[5]. JM Sung, HK Lee, and KJ Yang, Classification of Cordyceps spp. by morphological characteristics and protein banding pattern. Korean Journal of Mycology, 23, 1995, 92-104.
[6]. J Yang, W Zhang, P Shi, J Chen, and X Han, Effects of exopolysaccharide fraction (EPSF) from a cultivated Cordyceps sinensis fungus on c-Myc, c-Fos, and VEGF expression in B16 melanoma-bearing mice. Pathology Research and Practice, 201, 2005, 745-750.
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| Paper Type | : | Research Paper |
| Title | : | Antibacterial Activity of Leaf Methanolic Extract of S. Caryophyllatum (L.) Alston against Human Pathogenic Microorganisms |
| Country | : | India |
| Authors | : | Savitha Rabeque C || Jelly Louis |
Abstract:S.caryophyllatum (L.) Alston belongs to the family Myrtaceae is an endangered species. It possesses traditional as well as pharmacological properties. The objective of the present investigation was to find out the antibacterial activity of S. caryophyllatum leaf methanolic extract against some human pathogenic bacteria. It was followed by Disc Diffusion method using gram positive and gram negative bacterial strains such as Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Bacillus megaterium, Sarcina lutea, Esherichia coli, Pseudomonas aeruginosa, Klebsiella Spp., Salmonella typhi and Proteus mirabilis. The result showed that the inhibitory effect on Bacillus subtilis (24mm) was high when compared to E. coli (21mm) and Bacillus cereus (20mm).This effect on the bacterial strains may be due to the presence of secondary metabolites present in the leaf methanolic extract of Syzygium caryophyllatum.
Keywords: S. caryophyllatum, Bacillus subtilis, E. coli, Bacillus cereus.
[1]. T.M. Tsakala, O. Penge and K. John, Screening of in vitro antibacterial activity from Syzygium Guineense (Willd) hydrosoluble dry extract. Ann Pharm Fr, 54(6), 1996, 276-79.
[2]. G.D. Bagchi, A. Singh, S.P.S. Khanuja, R.P. Bansal, S.C. Singh and S.Kumar, Wide spectrum antibacterial and antifungal activities in the seeds of some coprophilous plants of north Indian plains. J Ethnopharmacol, 64, 1999, 69-77.
[3]. D.C.Djipa,M. Delmee and L.J. Quetin, Comparison of antimicrobial activities of Syzygium jambos (L.) extracts with other tannin containing plant extracts, J Ethnopharmacol,71, 2000, 307-13.
[4]. G. Nascimento, P. Locatelli, C. Freitas and G. Silva, Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J Microbiol,31, 2000, 247-56.
[5]. M. Berenbaum, Phototoxicity of plant secondary metabolites: Insect and mammalian perspectives, Arch Insect Biochem Physiol, 29, 1995, 119-34.
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| Paper Type | : | Research Paper |
| Title | : | United Arab Emirates Pharmacists' Practices and Views on Adverse Drug Reaction and Medication Error Reporting and Health Professionals' Expectations |
| Country | : | India |
| Authors | : | Majd Dameh |
Abstract: Adverse drug reactions (ADRs) constitute a huge burden on health systems, and medication errors (MEs) are the most common preventable cause of adverse drug events. In developed countries pharmacists contribute to a great extent in ADR monitoring and reporting, improving patient quality of care and safety. This review aims to explore pharmacists' practices and views on ADR reporting, extent and causes of MEs and other health professionals expectations of pharmacists in this regards. An extensive literature search was conducted using pertinent electronic health databases (ProQuest, PubMed, Embase, and International Pharmaceutical Abstracts, and the Cumulative Index to Nursing & Allied Health Literature). Hand-searching of the references retrieved was also performed.
1]. American Society of Health-System Pharmacists. ASHP guidelines on adverse drug reaction monitoring and reporting. Am J Health-Syst Pharm, 1995;52:417-419.
[2]. Aronson JK. Medication errors: definitions and classification. Br J Clin Pharmacol, 2009;67(6): 599–604.
[3]. Bates DW, Boyle DL, Vander MB, Schneider J, Leape L. Relationship between medication errors and adverse drug events. J Gen Intern Med. 1995;10:199–205.
[4]. Al Faouri IG, Hayajneh WA, Habboush DM. A five years retrospective study of reported medication incidents at a Jordanian teaching hospital: patterns and trends. Int J Humanit Soc Sci. 2014;4(7), 280-287.
[5]. United Arab Emirates, National Bureau of Statistics. UAE in figures 2010. (http://www.uaestatistics.gov.ae/English Home/LatestReportsDetailEnglish/ tabid/106/Default.aspx?ItemId=1925)