Paper Type |
: |
Research Paper |
Title |
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Therapeutic Efficiency of Aristolochic acid on Oral cancer
Induced Experimental Rats |
Country |
: |
India |
Authors |
: |
Mariappan Senthilkumar |
 |
: |
10.9790/3008-0421220  |
Abstract:Aristolochia indica is one of the important medicinal plant sources of bioactive compound
aristolochic acid (AA). The compound was found to produce cytotoxicity at higher doses, thus AA was examined
for therapeutic efficacy against 4-nitroquinoline 1-oxide (4-NQO) induced oral cancer. Purity of the isolated AA
was assayed using UV, IR, NMR, Mass spectral studies were revealed that the maximum wavelength (254 nm),
functional groups and nature (NO2 nitro groups and aromatic), the structure (C17H11NO7) and molecular weight
(341.28) of the compound AA. The dose of AA at 10 mg/kg b.wt/ for 4 weeks. Effect of AA on oral cancer was
studied by exploiting various biochemical and histological parameters such as Glutathione-S-transferase (GST),
Gamma gultamyl transferase (GGT), 5'-Nucleotidase (5'-NT) and Cathepsin D (CD) and glycoconjugates.
There was significant increase in GST and significant decrease in other markers and glycoconjugates were
observed on AA treatment in oral cancer induced rats. Number of tumors, percentage of tumors and tumor
volume was found to be decreased on AA treated rats on oral cancer induced rats. Thus AA was found to
modulate therapeutically on oral cancer regression.
Key Words: Aristolochia indica, Aristolochic acid, anti cancer, 4-Nitroquinoline-1-oxide, oral cancer,
therapeutic efficacy.
[1] F Gonzalez, Inflorescence morphology and the systematics of Aristolochiaceae. Syst. Geogr. Pl. 68, 1999, 159-172.
[2] Nortier JL, Mc. M. Martinez, H.H. Scheiser, V.M. Arlt, C.A. Bieler, M. Petein, M.F. Depierreux, L. De Pauw, D. Abramowitz, P.
Vereerstraeten, J.L. Vanherweghem, Urothelial carcinoma associated with the use of a Chinese herb (Aristolochia fangchi). New
England Journal of Medicine, 342, 2000, 1686-1692.
[3] M Stiborova, E. Freib, H.H. Schmeiser, Aristolochic acids and the kidney disease called Chinese herbs nephropathy. Chemicke
Listy, 94, 2000, 186-189.
[4] K Hashimoto, M. Higuchi, B. Makino, I. Sakakibara, M. Kubo, Y. Komatsu, M. Maruno, M. Okada, Quantitative analysis of
aristolochic acids, toxic compounds, contained in some medicinal plants. J. Ethnopharmacol., 64, 1999, 185-189.
[5] LT Angeles, B.D. Canlas, J.A. Concha, A.S. Sotto, P.L. Aligaen, Toxicity studies on aristolochic acid isolated from Aristolochia
Tagala, Cham. Acta Med Philipp., 6, 1970, 139-148.
[6] A Pakrashi, P. Pakrasi, Antifertility efficacy of the plant Aristolochia indica Linn on mouse. Contraception, 20, 1979, 49-54.
[7] CT Che, M.S. Ahmed, S.S. Kang, D.P. Waller, A.S. Bingel, A. Martin, P. Rajamahendran, N. Bunyapraphatsara, D.C. Lankin, G.A.
Cordell, Studies on Aristolochia III. Isolation and biological evaluation of constituents of Aristolochia indica roots for fertilityregulating
activity. Journal of Natural Products, 47, 1984, 331-341.
[8] T Ganguly, A. Pakrashi, A.K. Pal, Disruption of pregnancy in mouse by aristolic acid: I. Plausible explanation in relation to early
pregnancy events. Contraception, 34, 1986, 625-637.
[9] TS Wu, L.F. Ou, C.M. Teng, Aristolochic acids, aristolactam alkaloids and amides from Aristolochia kankauensis. Phytochemistry,
36, 1994, 1063-1068.
[10] W Martz, Plants with a reputation against snakebite. Toxicon, 30, 1992, 1131-1142.