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Paper Type	:	Research Paper
Title	:	Effect of Dietary Fibers on Blood Lipid Parameters in Hypertension Patients of Indore District
Country	:	india
Authors	:	Khurana N, Dhakad N
	:	10.9790/3008-00520104

Abstract: Each year, over three million deaths worldwide are tied directly to diabetes and even greater number die from cardiovascular disease. Modification in lifestyle and proper medication can delay and prevent cardiovascular diseases in high-risk groups. Eating whole grain carbohydrates and moderate exercise and avoiding excessive weight gain could eliminate over eighty per cent of cardiovascular diseases. The SITE study, sponsored by a multinational pharmaceutical company, Sanofi, tested almost 16,000 people living in urban cities across eight states over the last three years. It concluded that one in every five Indian adults living in urban cities suffers from hypertension and diabetes. Your blood pressure is the force exerted on your artery walls by the blood flowing through your body. A blood pressure reading provides two measures: Systolic Pressure and Diastolic Pressure .Both are expressed as millimeters of mercury (mm Hg).Systolic pressure is measured as the heart pumps. Diastolic pressure is measured between beats, as blood flows back into the heart. High blood pressure is often called the "silent killer" because it has no symptoms and can go undetected for years. It is important to have your blood pressure checked regularly. Dietary fiber (alternate names: roughage, bulk, diet-fiber) is a complex of substances of cell walls of plants which are not digested and not absorbed in a human's digestive tract. There are two major kind of dietary fibers Insoluble and Soluble type. Soluble and insoluble fiber act in totally different ways. Eating whole grain carbohydrates and moderate exercise and avoiding excessive weight gain could eliminate over eighty per cent of cardiovascular disease. This study is a clinical Trial with 40 non-smoker Patients which had no renal and liver disorder. The patients were selected through questionnaire data collection method, by ways of various health care camps organized in the city. They were randomly divided into 2 groups; the first group was given 30-45 grams of dietary fibers per day (intervention group). The control group remained normal with no additional dietary supplements. All the groups were observed for a period of2 years. Lipid parameters viz. HDL, LDL, TOTAL CHOLESTROL, TG was measured every year during the study for each group.Mean and SD were calculated. The significance of differences in the means of intervention group and control group were found using t test both paired and unpaired.
Keywords: cardiovascular disease, SITE study, Systolic Pressure, Diastolic Pressure, Lipid parameter, Dietary fiber s.

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Paper Type	:	Research Paper
Title	:	Determination of Maximum Herbaceous Production in Anyigba, a Derived Savanna
Country	:	Anyigba
Authors	:	Aina, D. O., Okayi, E. And Usman, S. S.
	:	10.9790/3008-0520509

Abstract :In this investigation, an attempt was made to estimate the herbaceous production or the carrying capacity of the derived Savanna vegetation of Anyigba area, Kogi State, Nigeria. Three plots of a hectare each were randomly marked out. Within the 3 plots (burnt, slashed and standing/control), 30 tussocks each were marked out and pegged for weekly observations of new flush or tiller regeneration for a period of six months. By random sampling, monthly fresh weights of herbaceous materials in a 1m2 quadrat were clipped and weighed with a spring balance in the field and oven dried to obtain the dry weights of the herbaceous materials. There was significant difference in herbaceous production between the plots. There was significant difference in tiller regeneration between the plots. Tiller regeneration was adduced to be responsible for corresponding herbaceous production. The implications of these in a derived savanna were discussed.
Key words: Quadrat, herbaceous materials, Derived Savanna, tiller regeneration, herbaceous production.

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Biological Sciences, University of Maiduguri, Nigeria.
[2] Abdullahi, M.B. and Sanusi, S.S. (2006). Phytosociological Survey of herbaceous vegetation of Gaji flood plains, Nigeria. Nigerian
Journal of Botany 19: 61-67.
[3] Afolayan, T.A. (1980). Range Management, Manipulation and Improvement in Kainji Lake Basin. Proceed. MAB-State-of
Knowledge Workshop on Nigerian Savanna, Kainji, Nigeria.
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grasses. East Afri. Wild L. J. 16: 97-104.
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diversity gradient and among land-use types. Ecol. Lett., 7: 684 – 694.
[6] Carr P.M., Poland, W.W. and Tisor L.J. (2005). Natural reseeding by forage legumes following wheat in Western North Dakota.
Agron. J., 97: 1270-1277.
[7] De-Leeuw, P.N. (1978). The Primary and Secondary Productivity of the Savanna in the middle belt of Nigeria. Paper presented at
Annual Conf. of the Nigeria Society of Animal Production. A.B.U., Zaria.
[8] Fagbenro, F.A. (1980). Effects of fire on soil with special reference to Tropical Africa. MAB-State-of-Knowledge Workshops on
Nigerian Savanna, Kainji, Nigeria.
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Oversees Government, UK. 109–211.
[10] Isichei, A.O. (1979). Elucidation of Nitrogen Stocks and Flows in some Nigerian Grassland ecosystems. Unpublished Ph.D. Thesis,
Univ. of Ife, Nigeria.

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Paper Type	:	Research Paper
Title	:	The anti-osteoporotic effect of Moringa oliefera on osteoblastic cells: SaOS 2
Country	:	India
Authors	:	Chirag Patel, Ayaz Rangrez, Pragna Parikh
	:	10.9790/3008-0521017

Abstract : M.oliefera oliefera (MO) is a middle-sized tree, cultivated throughout India. It is a multipurpose tree, used as a vegetable, spice, a source of cooking and cosmetic oil and as a medicinal plant. It is reported to contain alkaloids, flavonoids, anthocyanins. It possesses anti-inflammatory, antioxidant, antimicrobial, antihyperlipidaemic, antifertility and anticancer activities. An in-vivo study in our laboratory has proved the osteoprotective potential of the plant. In the present study an attempt is made to explore the osteoblastogenic potential of the different parts of the plant on the osteoblast cell line SaOS2. Methanolic extracts of MO components showed a positive effect, and of all the three components, flower and fruit were found to have significant osteoblast stimulating property. Flower extract was found to be increasing the number of osteoblastic cells; while the fruit extract was having more elaborative effect as it increased ALP activity, induced bone formation, increased hydroxyproline content and bone mineral formation. In conclusion, Methanolic Extact of MO on SaOS2 cell line affirmed the beneficial effects and thus indicates its potential in preventing osteoporosis.
Keywords- MO, MO Flower, MO Fruit, MO Leaves, Moringa oliefera, Osteoblasts, SaOS2

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Epimedium koreanum Nakai on the proliferation and differentiation of primary osteoblasts, Phytomedicine 15(1-2), 2008, 55–61.
[10] Vali B., Rao L.G., El-Sohemy A., Epigallocatechin-3-gallate increases the formation of mineralized bone nodules by human
osteoblast-like cells, Journal of Nutritional Biochemistry 18(5), 2007, 341 – 347.

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Paper Type	:	Research Paper
Title	:	Epidural Tramadol with low dose local anaesthetics for anaesthesia and postoperative analgesia in NYHA class III orthopaedic patients – A prospective randomized controlled study
Country	:	India
Authors	:	Paranjpe JS , Gaikwad SA , Patil MS, Bandgar SP, Deshpande TS
	:	10.9790/3008-0521822

Abstract : Aims & Objectives: 1.To assess the effect of epidural Tramadol with low dose local anaesthetics on quality of anaesthesia, postoperative analgesia, haemodynamic stability, and outcome in patients undergoing major orthopaedic surgeries on lower limb. Patients & Method: One hundred & seventy NYHA class III patients (age >60 yr) posted for major orthopaedic surgery on lower limbs during 1st July 2007 to 31st July 2010 were selected for the study. They were randomized into control (group A) & study group (group B). Group A received sixteen ml of an equal mixture of 1% Xylocaine and 0.25% Bupivacaine while Group B received Tramadol 50 mg in addition to the mixture of LAs epidurally. Inadequate level of sensory block necessitated further supplementation with 5 ml of 0.25% Bupivalocaine. For postoperative analgesia, group A received 5cc 0.125% (10 mg) Bupivacaine while group B received 5cc (50 mg) Tramadol.Anaesthetic, haemodynamic effects, pain scores, blood pressure, respiratory rate, side effects, complications and level of satisfaction were monitored for 72 hours. Adequacy of postoperative pain relief was judged by VAS. Statistical analyses were done by mean ± standard deviation. Correlation was examined with Pearson correlation coefficients (significance assumed with p < 0.05).
Keywords- Cardiac patient, Low dose epidural anaesthesia, Postoperative analgesia, Epidural Tramadol

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Anaesthesiol Clin Pharmacol 2011;27:139-41.
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Anaesthesia 2002;14:1-2.
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anesthesia in geriatric patients. Reg Anesth Pain Med 2003;28:215-20.
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Epidural Anesthesia and Analgesia on Stress Response and Immune Function in Patients Undergoing Radical Esophagectomy.
Anesth Analg 2005;101:1521-7
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major surgery: a randomized trial. Lancet 2002;359(9314):1276-82
[9] Chaturvedi S, Chaturvedi A. Postoperative pain and its management. Indian J Crit Care Med 2007;11:204-11.
[10] Susan M Nimmo. Benefit and outcome after epidural analgesia. Contin Educ Anaesth Crit Care Pain 2004;4:44-7.

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Paper Type	:	Research Paper
Title	:	The Isolation & Characterization of Several Strains of Naphthalene Degrading Bacteria obtained from oil-contaminated sites
Country	:	India
Authors	:	Sidhu.Navdeep, Rai.Yuvneet, Kaur.Maninder, Gupta.Ashna
	:	10.9790/3008-0522328

Abstract : Naphthalene is the simplest example of a PAH. PAHs occur in oil, coal, and tar deposits, and are produced as byproducts of fuel burning (whether fossil fuel or biomass). Common naphthalene degrading bacteria include Pseudomonas spp., Vibrio spp., Mycobacterium spp., Staphylococcus spp., and Sphingomonas spp. A well studied example of naphthalene catabolism is the naphthalene degradation (nah) pathway of Pseudomonas putida NCIB 98164. Five different isolates have been isolated which are Gram +ve bacteria and they all are naphthalene degrading by nature. It has been concluded that the bacterial strain capable of utilizing naphthalene is able to degrade it in 6 days. From HPLC analysis it is concluded that the concentration of naphthalene decreases as compared from blank solution which do not contain bacteria. HPLC analysis reveals that bacteria catabolises naphthalene within 6 days and decreases its absorbance due to less number of naphthalene present in the solution. Screening for relatively fast-growing naphthalene-degrading bacteria from different sites which were contaminated with crude oil resulted in the recovery of three isolates B. naphthovorans strain MN-003, Staphylococcus sp. strain MN-005 and Micrococcus sp. strain MN-006. Although the isolation methods were unbiased and could select for both Gram-positive and Gram-negative bacteria, all three candidate strains were Gram-positive. This dominance of Gram-positive bacteria is demonstrated in the high relative abundances of B.naphthovorans strain MN-003, Staphylococcus sp. strain MN-005 and Micrococcus sp. strain MN-006 in the enrichment culture.
Keywords-biomass, inoculum, gram staining, p-phenylenediamine, incubation, Bacillus; biodegradation, Gram-positive bacteria, Micrococcus, naphthalene

[1] Akhavan Sepahi. A., Dejban Golpasha. I., Nakhoda. A.M. Journal of Microbial World, 2009, 1(1), 5-14.
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Microbiology, 2005, 55 (4), 237-242.
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(2), 113117.
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Paper Type	:	Research Paper
Title	:	Comparative Study of Aqueous and Ethanolic Leaf Extracts Of Spondias Mombin on Neurobehaviour in Male Rats
Country	:	Nigeria
Authors	:	O. R Asuquo, C .E Fischer, O. E. Mesembe, A. O. Igiri, J .I Ekom
	:	10.9790/3008-0522935

Abstract : Remedies for anxiety and fear have been investigated through both orthodox and traditional medicine; this study compared the effects of chronic administration of graded doses of aqueous and ethanolic extracts of Spondias mombin on anxiety and fear in Albino Wistar rats. Fifty rats of Wistar strain (100-120g body weight) were divided into five major groups A-E with each group made up of ten rats (n=10). Spondias mombin (250, 400,500 and 800mg/kg) were orally administered for 4 weeks before testing. The elevated plus maze was used to assess anxiety behaviour. Following administration of graded doses of aqueous and ethanolic extracts of Spondias mombin for a period of four weeks, decreased anxiety and fear in the albino rats was revealed (p<0.05). The order of the anxiolytic property following these graded dose of administration was; duration and frequency in open arm of the 500mg/kg group was significantly higher (90.2 ± 37.2s & 4.4 ± 1.29/5min respectively) compared to control, and other treatment groups of Spondias mombin (P< 0.05).
Keywords-Spondias mombin, Fear, Anxiety, Frequency, Duration, Ethanol, Elevate plus maze.

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Paper Type	:	Research Paper
Title	:	"Medicinal Plants from the Landfill Site of Doon Valley"
Country	:	India
Authors	:	Seema Manwal, Dr. S.P. Joshi
	:	10.9790/3008-0523642

Abstract : Solid Waste generation and its disposal to safer sites are posing a threat to environment and human existence. Due to poor solid waste management practices, problems of health, sanitation and environmental degradation are unavoidable. In the current paper we have tried to assess the total Municipal Solid Waste (MSW) generated in Doon Valley and its disposal to landfill site along with the plant species that grow on this site. 116 plant species were recorded growing in landfill site. They included 2 tree species, 4 shrub species and remaining herb species. These included many invasive species like Lantana camara, Eupatorium adenophorum, Ageratum conyzoides and Parthenium hysterophorus.
Keywords-Phytoremediation, solid waste, Nagar Nigam, Landfill, MSW

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investigations. Proceedings of Sardinia ‟95, Fifth International Landfill symposium, S. Margherita di Pula, Italy, 2 -6 October, 1995.
[2] CPCB, 2000, Status of Municipal Solid Waste Generation, Collection Treatment, and Disposal in Class 1 Cities, Central Pollution
Control Board, Ministry of Environment and Forests, Government of India, New Delhi.
[3] NEERI, 1995, "Strategy Paper on SWM in India‟, National Environmental Engineering Research Institute, Nagpur.

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Paper Type	:	Research Paper
Title	:	Prevalence of Risk Factors of Non-Communicable Diseases amongst Female Prisoners of Pakistan
Country	:	Pakistan
Authors	:	Salina Mukhtar, Atif Mahmood, Asima Faisal, Saima Ejaz, Fahmida Khatoon
	:	10.9790/3008-0524348

Abstract : Objective: To determine the prevalence and the differences in the prevalence of risk factors for noncommunicable diseases among the female prisoners of all four provinces of Pakistan. Method: Cross-sectional study which was conducted at the Female Prison of Karachi (Sindh), Koth Lakh Patt (Punjab), Quetta (Baluchistan) and Haripur Jail (Khyber Pakhtoon Khuwah). Sample size was determined by the rule of thumb and 269 adult female prisoners of all the four Prisons (66% population of each prison) were recruited, thereby, determining one prison of each province of Pakistan, the age of whom ranged from 16 years and onwards. The blood pressure, random blood sugar and anthropometric measurements including height, weight, BMI and waist hip circumference of the prisoners were performed.
Keywords-Prevalence; Non-Communicable diseases; Risk factors; Pakistani Female Prisoners; Prevalence

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efforts. Addiction, 2004 Jul;29(5):1015-1019
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[10] Robertson P.B, Walsh M.M, Greene J.C. Oral effects of smokeless tobacco use, Advances in Dental Research 1997;11(3):307-312.

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Paper Type	:	Research Paper
Title	:	Chronic Smoking and Buerger's Disease: A Hospital Based Study
Country	:	India
Authors	:	Shamim Akhtar , Gumashta Jyotsna, Gumashta Raghvendra
	:	10.9790/3008-0524954

Abstract : Introduction: The role of tobacco smoking and Buerger's Disease has been examined in different countries. However, still there is need to understand the basic pathology and the conclusive important reasons of its causation because of differing observations among smokers of various socio-economic and geopolitical settings. Hence, the study highlights the scenario, as observed in a developing country, for further designing and undertaking of the research in this regards. Purpose: To assess the prevalence of Buerger's Disease among various groups of traditionally used locally manufactured bidi smokers.
Keywords-Angiography; Buerger's Disease; Thromboangitis obliterans; Venography

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(Buerger's disease). Circulation. 1990 Nov;82(5 Suppl):IV3-8.
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England 1992; 74:115-118.
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(Torino). 1980 Jan-Feb;21(1):77-84.
[7] Szuba, Cooke. An Update on Buerger's Disease. WJM, Apr1998;168(4):256.

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Paper Type	:	Research Paper
Title	:	Impact of Co-Processing on Some Fundamental Physicochemical and Functional Properties of Microcrystalline Cellulose
Country	:	Nigeria.
Authors	:	Philip F. Builders, Kabele-Toge B., Adarki W. Pongri , Oluranti D.
	:	10.9790/3008-0525567

Abstract : The aim of this study was to evaluate the effect of co-processing of microcrystalline cellulose (MCC) with Eudragit L100(R), cellulose acetate phthalate and ethyl cellulose respectively on the compaction, flow and disintegration properties of MCC. Co-processing MCC with the different polymers enhanced the flow and disintegration properties of MCC as a direct compression excipient for tablets production. When evaluated with the Heckle model, there was no remarkable change in the packing characteristics of the modified motifs in relation to the unmodified; however there was a slight but variable decrease in dilution capacity. This study shows the potential attribute of co-processing MCC with other polymers.
Keywords-Microcrystalline cellulose, Eudragit L100, cellulose acetate phthalate, ethyl cellulose, coprocessing

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Paper Type	:	Research Paper
Title	:	Protective Effects of Cool Extraction of Black Seed (Nigella Sativa) Oil Against CCl4-Induced Oxidative Damages in Wistar Rats Testis
Country	:	Nigeria
Authors	:	J. Danladi, Ahmed S. A, Akpulu S. P, Owolagba G. K, Iduh M. U, Mairiga A. A
	:	10.9790/3008-0526874

Abstract : Over many centuries humans have been mining the bounties of nature for discovering substances that have been used for the treatment of all human diseases. Traditionally various human diseases of kidneys, hormonal imbalance and sexual diseases are treated with Nigella sativa oil. In the present study, protective effects of cool extraction of Nigella sativa oil was evaluated against CCl4-induced oxidative damages in rat testis. To examine the protective effects of cool extraction of Nigella sativa oil on testis against oxidative stress of carbon tetrachloride in male rats, 35 male albino rats were equally divided into 7 groups (5 rats). Group 1 rats were administered normal Saline (volume per body weight) orally. Group 2 rats were administered olive oil 4ml/kg body weight orally. Group 3 rats were administered 2ml/kg body weight of N. sativa oil orally. Group 4 rats were administered 4ml/kg body weight of N. sativa oil orally for 2 weeks. Group 5 rats were administered 4ml/kg body weight CCl4 (30% CCl4 in 70% olive oil) orally. Group 6 rats were administered 2ml/kg body weight of N. sativa oil plus 4ml/kg body weight CCl4 (30% CCl4 in 70% olive oil) orally. Group 7 rats were administered 4ml/kg body weight of N. sativa oil plus 4ml/kg body weight CCl4 (30% CCl4 in 70% olive oil) orally. Protective effects of Nigella sativa oil were observed on the biochmical (SOD, CAT, GSH and LPO) and histological paramters. Results of the present study revealed that treatment of CCl4 caused elevated level of TBARS and significantly reduced sperm concentration comparatively to control. N. sativa treatment positively protects the alterations in biochemical variables SOD, CAT, GSH and LPO in the CCl4 + N. sativa-treated rats. N. sativa markedly reduced elevated TBARS. Histopathological changes were observed. Testis tissues in group 1, 2, 3 and 4 showed normal testicular architecture. There was a widespread degeneration in the spermatogenic cell lines of the seminiferous epithelium of group 5 rats. N .sativa oil showed protective effect on the histological section of the testis in groups 6 and 7. From the results it is suggested that cool extraction of Nigella sativa oil extract has the ability to protect testis against oxidative and histological damages, possibly through antioxidant effects of its bioactive compounds.
Keywords-CCl4, N. sativa, Biochemical Assay, Testis, Wistar Rats

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Paper Type	:	Research Paper
Title	:	Determination of Chemoattractant factor (Interleukin -8) and Gamma interferon (IFN- γ) among Lymphoma patients (Hodgkin's and Non Hodgkin).
Country	:	Iraq
Authors	:	Raheem Tuama O. AL–Mammori , Dr . Azhar Emran AL-Thahab . Dr . Alaa Sadeq Al-Awad.
	:	10.9790/3008-0527578

Abstract : Fifty (50) patients clinically diagnosed as lymphoma patients, 17 (34%) Hodgkin's and, 33 (66%) patients Non Hodgkin ,attending Tumor Center in Merjan medical city, and (30 ) matched age person, as a control group enrolled in this study in the period between Junuary and Augest 2012. Specific diagnosis is clinically done by consultant physician, histopathologist and confirmed by specific marker or Cluster of Differentiation (CD) markers (CD20 and CD30), IL-8, and IFN-γ, estimated for all patients and control. The result shows that a highly significant (P<0.01) increased in IFN- γ in both groups HL, and NHL, compared with control . IL-8 shows highly significant decreased (P<0.01) in result of NHL, compared with control, as well as no significant decreased of HL, in comparison with control. There is significant increased (P<0.05) in IL-8 level of HL patients compared with NHL. The Immunohistochemical result shows that highly association between CD20 with NHL, CD30 with HL. And it used specifically to confirm histopathological diagnosis of lymphomas. From present study can concluding the facts that, Activation of B and T cells in lymphoma patients lead to increased anti proliferating activity and immunomodulary of IFN- γ, and in less extent IL-8 as chemotactic factor. Cytokines level important to monitored the severity of damage and whether the patients with good or bad prognosis. So that recommended to study other different types of cytokines for Th1, Th2, and NK cell activators to predict of ability to producing pro-inflammatory and anti-inflammatory mediators as well as ability to treatment of such diseases.
Keywords-Chemotactic Factor. Hodgkin's lymphoma, Interleukin-8 , Interferon – γ , Non Hodgkin's lymphoma

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Medical Center". Yonsei Med. J. 49 (4), 2008, 601–9.
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Paper Type	:	Research Paper
Title	:	Threat Assessment Of Medicinal Plants Of Koria District In Chhattisgarh (India).
Country	:	India
Authors	:	Mantosh Kumar Sinha
	:	10.9790/3008-0527986

Abstract : Koria district of Chhattisgarh state has a very rich plant diversity specially of medicinal plants and there is no comprehensive description of the flora as well as vascular cryptogames of the district is available some plant species are on the verge of extinction..The objective of the study was threat assessment of medicinal plants of Koria district based on the criteria developed by the IUCN. Keeping these point in view the present investigation was planned to assess the biobiversity of the vegetation of the district.Extensive field surveys were undertaken during 2004-08 Sampling sites were selected randomly covering all the blocks. Quantitative phytosociological characters were studied as per methods described by Misra (1968). The threat categories in the present study have been prepared viewing to the medicinal plants found in the wild only. Assessment of threat was done following the guidelines of IUCN (2000) Study reveals that 3 medicinal plant taxa were found to be regionally extinct in wild(Ex.) ,14 were critically endangered(CR), 27 were endangered (EN),62 were vulnerable (VU) and 37 were near threatened (NT) and 57 were least concerned(LC).
Keywords-Threat assessment,Koria district, Medicinal plants

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129(9)2003,1090-1108.
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Forester, 129(1) 2003,55-68.
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to analyse the mini forest ecosystem. Indian Foreser 131(6) ,2005,773-785.
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[9] Udayan, P.S. ,Notes on a few rare, endemic and red-listed species from High Range (Mannavanshola) near Munnar, Idukki
district, Kerala state. Indian Forester 132(11) ,2006, 1453-1464.
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forests of Meghalaya, north-east India: Population structure and regeneration efficacy of some important species. Curr. Sci.
90(2) 2006, 225-232.

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Paper Type	:	Research Paper
Title	:	L- Carnitine Decreases Her-2/neu in Breast Cancer Patients Treated with Tamoxifen
Country	:	Egypt
Authors	:	Rania M. Khalil, Hoda A. El-Bahrawy , Nahla E. El-Ashmawy , Hossam Darwis
	:	10.9790/3008-0529198

Abstract : The crosstalk between the estrogen receptor (ER) and the growth factor receptor pathways is implicated in breast cancer cell growth. L-carnitine as complementary medicine may help reducing therapy-associated toxicity or improving cancer-related symptoms. The possible role of L-carnitine to modulate IGF-1 and Her-2/neu in breast cancer patients maintained on tamoxifen, was investigated.
Keywords-Breast cancer, Her-2/neu, IGF-1, L-carnitine, Tamoxifen

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Barrett and P.V. Schoenlein, A role for macroautophagy in protection against 4-hydroxytamoxifen-induced cell death and the
development of antiestrogen resistance, Mol Cancer Ther, 7(9), 2008, 2977-2987.
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Paper Type	:	Research Paper
Title	:	Molecular Cloning and Characterization of a Chitinase Gene from the Thermophilic Fungus, Thermomyces lanuginosus-RMB
Country	:	India
Authors	:	Peramachi Palanivelu , Lakshmi Vaishna
	:	10.9790/3008-05299107

Abstract : A chitinase gene from the thermophilic fungus, Thermomyces lanuginosus was amplified from the genomic DNA of the fungus, by PCR technique. The PCR product was cloned into pXcmkn12 vector by TA cloning technique and sequenced. The gene contained five introns and six exons. The deduced protein sequence coded for 390 amino acids and exhibited very high degree of similarity to other endochitinases of mesophilic and thermophilic fungi. The highly conserved catalytic motif, DGLDIDWEYP, suggests that it belongs to family 18 chitinases.

Keywords: Thermophilic fungus, Thermomyces lanuginosus, Family 18 Chitinase, Introns

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Paper Type	:	Research Paper
Title	:	In vitro Embryo culture of Croton scabiosus Bedd. (Euphorbiaceae), an endemic plant of Southern Andhra Pradesh
Country	:	India
Authors	:	S. Salamma, B. Ravi Prasad Rao
	:	10.9790/3008-052108114

Abstract : Croton scabiosus Bedd. (Euphorbiaceae) is a global endemic to Southern Eastern Ghats of Andhra Pradesh, India. Seed pathological problems, poor germination and recurrent fires in the native habitat are the major causes for low regeneration of this species. With an objective to augment the species population in the natural and allied habitats, the present study focus on developing a standard protocol for mass multiplication of the species through embryo culture. Of the various growth regulators employed for embryo culture for the species, 0.5mg/l BAP+ 3.0mg/l IAA has shown the maximum response and produced healthy plantlets.

Key words - Croton scabiosus, embryo culture, mass multiplication, population augmentation.

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Paper Type	:	Research Paper
Title	:	Optimization of Fungal Rennet Production by local isolate of Rhizomucor nainitalensis Under Solid Substrate Fermentation system
Country	:	Iran
Authors	:	Khademi F., Abachi S., Mortazavi A., Ehsani M.A., Tabatabaei M.R., Malekzadeh F.A.
	:	10.9790/3008-052115121

Abstract : In this study the production of substitute rennet by a newly isolated thermophilic fungus, Rhizomucor nainitalensis, using optimized solid substrate fermentation system have been explored. The results revealed that enzyme activity is at its highest level at 40˚C, 50 % humidity, inoculation volume of 103 spore /gr wheat bran, and exoenzyme biosynthesis induction by casein at a rate of 1.5 %. Enzyme activity reached its highest (8400 SU/gr wheat bran) after 110 hours of incubation under the stated conditions. Precipitated enzyme, by addition of ammonium sulfate with milk coagulating activity of 115000 SU/gr enzyme powder, was free of toxin and microorganisms, with market's favorable aroma.

Keywords- Rhizomucor, Solid Substrate Fermentation, rennin, fungal proteases

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Paper Type	:	Research Paper
Title	:	Comparative Studies on Antidiabetic effect with phytochemical screening of Azadirachta indicia and Andrographis paniculata
Country	:	Bangladesh
Authors	:	Rasheda Akter, M Mahabub-Uz-Zaman, Md. Saidur Rahman, Most. Afroza Khatun, A. M. Abdullah, Nazim Uddin Ahmed, Faridul Islam
	:	10.9790/3008-052122128

Abstract : The present study was undertaken to evaluate the antidiabetic properties with phytochemical screening of the ethanol leaves extracts of two common indigenous medicinal plants of Bangladesh -Azadirachta indica (Neem) and Andrographis peniculata (Kalomegh) on experimental animal model. Hypoglycemic activity of the concentrated ethanolic (90%) extract of Azadirachta indica and Andrographis peniculata were studied compared with that of a reference antidiabetic drug glimeperide in both glucose loaded and alloxan induced diabetic rats. The acute toxicity of these two plants extract was also studied. Experimental results showed that ethanol leaves extract (1 gm/kg) of Azadirachta indica and Andrographis peniculata significantly (P<0.01) reduced the elevated blood glucose level by 36.91 % and 40.65 % respectively in glucose loaded rats and 30.20 % and 32.18 % respectively in alloxan induced diabetic rats compared to the respective diabetic control group. This study strongly suggested that ethanol leaves extracts of Azadirachta indica and Andrographis peniculata possess significant antidiabetic activity and could be a potential source for treatment of diabetes mellitus. Key words: Azadirachta indica, Andrographis peniculata, Diabetes mellitus, Alloxan induced diabetes.

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331-332, 391-393

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iosr-JPBS Volume-5 ~ Issue-2

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23

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10

600

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Volume-5 ~ Issue-2