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| Paper Type | : | Research Paper |
| Title | : | Detection of Alpha-Amylase Activity from Soil Bacteria |
| Country | : | Nigeria |
| Authors | : | Salihu, Y. || Saidu, A. Y. || Rabiu, G. A. || Umar A.A. || Zeynep, A. || Unzile, G. |
Abstract: Background: Alpha-amylase is one of the industrial enzymes that hydrolyze starch molecules into polymers composed of glucose units. The enzyme has potential application in a wide number of industrial processes such as food, textile, paper, detergent, fermentation and pharmaceutical industries. Alpha-amylase can be produced by microorganisms, plants or animals. Aim: The aim of this study is to detect the activity of alpha-amylase from bacteria isolated from soil environment.
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| Paper Type | : | Research Paper |
| Title | : | DNA construct instability in bacteria used for Agrobacterium mediated plant transformation |
| Country | : | Nigeria |
| Authors | : | Ihuoma C. Okwuonu || Ome K. Achi || Uchechi N. Ekwenye || Solomon O. Afuape || Francis A.Nkaa || Chiedozie N Egesi. |
Abstract: The use of plasmid in the production of genetically modified (GM) crops is highly essential in research and in commercial production of GM plants. However plasmid instability constitutes a major problem in the use of recombined microorganisms in the production of GM crops. In this study we evaluated the stability of p8114 carrying a gene coding for a transcription factor (TFIIIA) driven by Cassava Vein Mosaic Virus (CsVMV) promoter and an nptII selectable marker driven by 35S promoter in the T-DNA. The plasmid was amplified in E.coliDH5α strain on Luria Broth (LB)agar supplemented with 100 μg/ml kanamycin. The colonies were confirmed by Restriction Fragment Length Analysis (RFLA) and by DNA sequencing. The confirmed colonies were stored as glycerol stock at -800C and as DNA extracts in TE buffer at 40C. Agrobacterium strains LBA4404, EHA 105 and AGL1 were also transformed with DNA from the confirmed colonies. Plasmid stability was evaluated after 3 months.
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| Paper Type | : | Research Paper |
| Title | : | Polyesters From Chemically Modified Oil of Renewable Source |
| Country | : | Nigeria |
| Authors | : | O.FOsagie || Oriji A.B |
Abstract: Rubber seeds, agricultural wastes without any known nutritional value, were blended to obtain oil. The oil was employed in the production of polyesters (alkyd resins)usingalcoholysis method. The rubber seed oil (RSO) and its alkyd resins were characterized and evaluated by standard methods for their physicochemical properties, drying schedule and chemical endurance. RSO had an iodine value of 99.40, saponification values of 202.36, acid value of 22.16, viscosity of 25.72 free fatty acid of 11.20 and golden yellow colour. Also, the RSO prepared polyesters have coating properties whose values are comparable to those of commercial polyesters. It is therefore recommended that the oil obtained from the seeds of natural rubber (agricultural waste) be exploited in Nigeria for the production of environmentally friendly polyesters in other to optimize its application in non pigmented surface coating.
Keywords: Wastes, Rubber seeds, Environmentally friendly, Renewable source, polyesters
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