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| Paper Type | : | Research Paper |
| Title | : | Production and Molecular Characterization of riboflavingenic Strain of Aashbya gossypii |
| Country | : | Khartoum sudan |
| Authors | : | Shami E. A. Bakhiet and Engaz Mustafa Ibrahim Mohamed |
Abstract: Riboflavin, a yellow, water soluble solid, is wide distributed in plants and animals, and plays an important role in live organisms because it is the precursor of flavin mononucleotide (riboflavin 5'-monophosphate, FMN) and flavin adenine di-nucleotide (FAD), which are functioning as coenzymes for wide variety of enzymes in intermediate metabolism. The main objective of the current study is to produce riboflavin by Ashbya gossypii, estimate the concentration of riboflavin and molecular characterization and differentiation between riboflavinogenic and non-riboflavinogenic strains....
Key words: Ashbya gossypii, Flavin adenine di-nucleotide, Riboflavin, riboflavinogenic, Spectrophotometer..
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| Paper Type | : | Research Paper |
| Title | : | Cloning, Expression and Purification of Chalcone Synthase from Solanum Tuberosum |
| Country | : | India |
| Authors | : | Pulak Das || Shuban K Rawal |
Abstract: Chalcone synthase (CHS) gene was amplified by PCR from cDNA of Solanum tuberosum leaf tissue. The gene was cloned into pGEMT easy vector and sequenced. It was then ligated into pET 30 vector to construct recombinant plasmid pET30-CHS. The recombinant was transformed into E.coli BL21 strain and the target gene was over expressed using IPTG. Optimum condition of IPTG concentration and temperature for over expression was found to be 1 mmol L-1 and 37ºC respectively. The results showed that the His-tag- CHS was highly heterogonous expressed in the inclusion bodies into the host cell. However, the His-tag- CHS fusion protein was purified using affinity chromatography with Ni2+-NTA resin column. SDS-PAGE analysis demonstrated that the host with pET30-CHS generated a 42 kD His-tag-CHS fusion protein.
Key words:Chalcone synthase; fusion protein; plasmid; cloning; purification; expression.
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| Paper Type | : | Research Paper |
| Title | : | Bcl-2(Rs956572) G/A Mutation in Cigarette Smokers and receptiveness to Lung Cancer |
| Country | : | Saudi Arabia. |
| Authors | : | Dilshad Ahmad |
Abstract: Cigarette smokingis the root cause of cancer and death from lung cancer. Singlenucleotidepolymorphisms(SNPs) are the salient location in an individual genome which influences the diseases. Since cigarette smoke contain large number of oxidants which may induce mutations. Bcl-2 SNPs have been found to be associated with predisposition of different cancers. In present study we examine the association of bcl-2 variant rs956572 with the cigarette smokers to predict the risk of smoking related cancers in smokers.....
Key words: Bcl-2, (rs956572) G, smokers, lung cancer, Single nucleotide polymorphisms
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