Version-1 (May–June 2017)
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| Paper Type | : | Research Paper |
| Title | : | Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection |
| Country | : | Egypt |
| Authors | : | A.B. Abdel-Razik || I.A. Hammad || E. Tawfik |
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: | 10.9790/264X-03030113  |
Abstract: Two thionin genes were isolated from Arabidopsis thaliana (AT1G12660 and AT1G12663). The resulted thionin protein has antifungal activity against potato pathogenic fungi like Alternaria alternata and Rhizoctonia solani. These two thionin genes were respectively inserted in pEGAD vector. After that carried into chitosan nanoparticle in order to be transformed into the two tested potato cultivars (lady and spunta) using intermodal cutting technique of tissue culture. Expressions of thionin transgenes in the transgenic plants were confirmed by RT-PCR. There were two assays were applied to test the role of thionins (in the transgenic potatoes) in resistance to the pathogenic fungi: effect of spore suspension on potato organs..............
Key words: Alternaria alternata, chitosan, Rhizoctonia solani, Solanum tuberosum, Thionin gene, Transformation
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| Paper Type | : | Research Paper |
| Title | : | Comparison Between Follicular Antimüllerianhormonelevels Between Women's Withpolycystic Ovarian Syndrome And Normal Ovulatory Women Undergoing Intracytoplasmic Sperm Injection (ICSI) Techniques |
| Country | : | Egypt |
| Authors | : | Inam Abdul- Wahid || Affan E Hassan || Basil O Saleh || ZeyadTareq Abdul Razzaq |
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: | 10.9790/264X-03031418 |
Abstract: AMH is a glycoprotein of the transforming growth factor-β (TGF- β) superfamily; it has as an important role in regulation of the ovarian follicle development.AMH Produced by granulosa cells of growing follicles after birth.Polycystic ovary syndrome (PCOS) is the most common cause of menstrual dysfunction, infertility, and hyperandrogenism, and itsprevalence is about 5-10% in female population of reproductive-age women. Follicular fluid (FF) surrounding the oocyte may play an important role in determining oocyte quality and the future potential to achieve fertilization and development of embryo and it provides a very good environment for the development of the oocytes...............
Key words: AHM, PCOS, follicular fluid, ICSI.
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| Paper Type | : | Research Paper |
| Title | : | Application of Sodium Alginate Hydrogel |
| Country | : | China |
| Authors | : | Wael jumah Aljohani || li wenchao || Muhammad Wajid Ullah || Xianglin Zhang || Guang Yang |
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: | 10.9790/264X-03031931 |
Abstract: Tissue engineering and 3D cell cutler involving the use of three-dimensional scahffold for cell and tissue cultural ,is a promising technique for establishing in vitro culture models that mimc in vivo environment . In vitro models present ethical and cost advantages over in vivo models, and allow for tissue devolpmint.alginate , aliner polysaccharide derived from brown algae .has properties that make it a favorable materials as a 3D extracellular matrix for in vitro cell and tissue models and some of sodium Hydrogyl materials are higly appealing as 3D scaffold for in vitro 3D cell cutlre and tissue culture due their ability to mimic the physical properties native tissue as well as their high porosity for effcient diffusion of proteins and nutrients Alginate hydrogel is widely ysed in tissue engineering application due to its desirable propertis as Biomaterials .
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