Version-1 (Sep–Oct 2017)
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| Paper Type | : | Research Paper |
| Title | : | Correlation between liver enzymes and lipid profile in type II diabetes mellitus – A case control study |
| Country | : | India |
| Authors | : | Sangappa Virupaxappa Kashinakunti || Manjula Rangappa || Gurupadappa Shantappa Kallaganada |
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: | 10.9790/264X-03050105  |
Abstract:Background: Liver is the critical site for carbohydrates and lipid metabolism. Any alternation in liver function may lead to altered metabolism of both glucose and lipids. Hence relationship must exist between lipid profile and liver function tests. Many studies showed that metabolically type II diabetes mellitus patients have hyperlipoproteinemia. Objectives: Hence the present study was undertaken to study the comparison and correlation between liver enzymes and lipid profile in type 2 diabetes mellitus patients. Material and methods: The study was conducted at HSK hospital, Bagalkot. Fifty subjects participated in type II diabetes mellitus patients and Controls. Biochemical parameters like fasting blood glucose, urea, creatinine, lipid profile, liver enzymes and HbA1c were estimated............
Key words: Diabetes mellitus, Lipid profile, Liver enzymes
[1] Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes 2015; 6(3): 456-480 Available from: URL: http://www.wjgnet. com/1948-9358/full/v6/i3/456.htm DOI: http://dx.doi.org/10.4239/wjd.v6.i3.456
[2] King GL. The role of inflammatory cytokines in the diabetes and its complications. J Periodontal 2008;(1):1527-34.
[3] Kumar KPS. Bhowmik D, Srivastava S, Paswan S, Dutta AS. Diabetes epidemic in India-A comprehensive review of clinical features, management and remedies. The pharma innovation. 2012;2:17-33.
[4] Clouston A, Powell E. Nonalcoholic fatty liver disease: Is all the fat bad? Intern Med J 2004; 34: 187-191.
[5] Hanley AJ, Williams K, Festa A, Wagenknecht LE, Kempf J, ZinmanB, Haffner SM. Elevations in marker of liver injuryand risk of type 2 diabetes-The insulin resistance atherosclerosis study. Diabetes 2004; 53: 2623-2632..
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| Paper Type | : | Research Paper |
| Title | : | Assessment of toxicity in dairy waste: A Review |
| Country | : | India |
| Authors | : | Gour Suman || Mathur Nupur || Singh Anuradha || Bhatnagar Pradeep |
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: | 10.9790/264X-03050610 |
Abstract: Globally, the dairy sector is one of the most important sectors of the world. The milk processing industry is emerging during the last two decades due to enormous increase in the milk production and increase in demand for milk and milk products. The dairy industry involves processing raw milk into products such as consumer milk, butter, cheese, yogurt, condensed milk, dried milk (milk powder), and ice cream, using processes such as chilling, pasteurization, and homogenization. The typical by-products of milk are buttermilk, whey, and their derivatives. The effluents are generated from milk processing through milk spillage, drippings, washing of cans, tankers bottles, utensil, and equipment's............
Key words: Dairy waste, BOD, COD, toxicity, effluent
[1] Arumugam, A.and Sabarethinam, P. L.( 2008).Performance of a three-phase fluidized bed reactor with different support particles in treatment of dairy wastewater‖ ARPN Journal of Engineering and Applied Sciences 3(5): 42-44.
[2] Bharati S.S and Shinkar N. P.( 2013).Dairy Industry Wastewater Sources, Characteristics & its Effects on Environment International Journal of Current Engineering and Technology 3(5):1611-1615.
[3] Noorjahan, C.M., Dawood, S., Sharief and Dawood, N .(2004)Characterization of dairy effluent Jr. of Industrial Pollution Control 20 (1): 131 -136.
[4] Deshannavar U. B., Basavaraj. R. K and Nandini M. Naik, (2012).High rate digestion of dairy industry effluent by upflow anaerobic fixed-bed reactor‖, Journal of Chemical and Pharmaceutical Research. 4(6):2895-2899.
[5] Deshpande D.P., Patil P.J. and Anekar S.V. (2012).Biomethanation of Dairy Waste‖, Research Journal of Chemical Sciences, 2(4): 35-39..
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| Paper Type | : | Research Paper |
| Title | : | Genetic diversity of Muscovy ducks revealed by mtDNA d-loop |
| Country | : | India |
| Authors | : | Kameshpandian Paramasivam || Satyanarayana Swamy Vyshnava || Dileep Kumar Kanderi || Cino Pertoldi |
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: | 10.9790/264X-03051118 |
Abstract: Based on geographical location and previous studies, the Muscovy ducks were grouped as six different populations such as India-Manipuri (IM), Wuyi-China (WC), Yuyao-China (YC), Fujian-China (FC) and Unkown-China (UC) and France. In total, 12 haplotypes were observed from six Muscovy population. The India-Manipuri (IM) population contributes 8 haplotypes. The AMOVA test shows high (75.12%) genetic variation within population. The NJ phylogenetic tree shows the intermingled China, India and France Muscovy populations. In order to find the depth of haplotype differences, the median joining network was constructed whereby H1 haplotype were shared by India and China population. Moreover, except H1 haplotype, other seven haplotypes from IM population were not...........
Key words: Muscovy, AMOVA, NJ phylogenetic tree, MJ network, NMDS
[1] Donkin RA (1989) The Muscovy Duck, Cairina moschata domestica: origins, dispersal, and associated aspects of the geography of domestication. Rotterdam: AA Balkema.
[2] Stahl PW, Muse MC, Espinoza FD (2006) New evidence for pre-Columbian Muscovy duck Cairina moschata from Ecuador. IBIS 148: 657–663.
[3] Stahl PW, Norton P (1987) Precolumbian animal domesticates from Salango, Ecuador. Am Antiquity 52 (2): 382-391.
[4] Stahl PW (2005) An exploratory osteological study of the muscovy duck (Cairina moschata) (Aves: Anatidae) with implications for neotropical archaeology. J Archaeol Sci 32: 915-929.
[5] Stahl PW (2003) Pre-Columbian Andean animal domesticates at the edge of empire. World Archaeol 34 (3): 470-483...
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| Paper Type | : | Research Paper |
| Title | : | Genotyping of A251g, Copper-Zinc Superoxide Dismutase (Cuznsod) Polymorphism, in Term Pregnant Women In Uyo, South-South, Nigeria |
| Country | : | Nigeria |
| Authors | : | Akpet Imaobong || Anwana Ubong || Samuel Glory || Eka Offiong || Ndodo Nnaemeka D |
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: | 10.9790/264X-03051922 |
Abstract: Cu/ZnSOD protein and its gene are located on chromosome 21q22. Cu/ZnSOD is found almost exclusively in intracellular cytoplasmic spaces and comprises 90% of total SOD making the investigation of Cu/ZnSOD expression levels has become very important. The A251G Copper-Zinc Superoxide Dismutase polymorphism is distributed widely among south -south pregnant Nigerian population, showing the prevalence of the wild type. Fifty apparently healthy subjects were recruited from St. Luke's Hospital Anua, Akwa-Ibom State, Nigeria during the antenatal clinic. Genotyping of A251G.........
Key words: A251G Copper Zinc Superoxide Dismutase; PCR; RFLP, Genotyping
[1]. Asaduzzaman Khan, M., Tania, M., Zhang, D. Z. and Chen, H. C. (2010). Antioxidant Enzymes and Cancer. Chinese Journal of Cancer Research, 22(2): 87-92.
[2]. Hong, Y.C., Lee, K.H., Yi, C.H., Ha, E.H. and Christiani, D. C. (2002). Genetic Susceptibility of Term Pregnant Women to Oxidative Damage. Toxicology Letters, 129(3): 255 – 262. [3]. Nithya, K., Angeline, T., Isabel, W., & Asirvatham, A. J. (2016). SOD1 Gene.Genetics research international, 2016.
[4]. Noor, R., Mittal, S. and Iqbal, J. (2002). Superoxide Dismutase-Applications and Relevance to Human Diseases. Medical Science Monitor 8: 210-215.
[5]. Osredkar, J. (2012). Copper and Zinc, Biological Role and Significance of Copper/Zinc Imbalance. Journal of Clinical Toxicology, 2013, 3-18....
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| Paper Type | : | Research Paper |
| Title | : | Evaluation of Spectrometric Characterization of Bioactive Compounds in Hibiscus Rosa-Sinesis |
| Country | : | Nigeria. |
| Authors | : | Ogbuji C A. || Ndulaka J.C. || Eke Ugorji I. || Ogbonna, C.R |
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: | 10.9790/264X-03052327 |
Abstract: Infrared Spectrophotometric technique was employed to characterize some bioactive compounds present in the leaf extracts of Hibiscus rosa-sinensis. The compounds characterized were 2- thenoyltrifluoroacetophenone, p-tolyacetonitrile, 1,2,4- trichloroacetophenone, 4-chloroacetophenone, ethyl-4-chloro-2-cyanoacetoacetate,2,4,6-trihydroxyacetophenon,ethyl-4,4,4- trichloroacetoacetate, 1-acetonaphthane, and 3-indoleacetontrile were characterized in Hibiscus rosa-sinensis. The presence of these bioactive compounds in these herbs may be the key to their suggestive efficacy and application in ethno-medicine.
Key words: Bioactive compounds, infrared spectroscopy, Spectrometric, Hibiscus rosa-sinensis.
[1] Aksel, B. (2010). A Brief Review on Bioactive Compounds in Plants. Norwegian Acad. Sci. Lett. 10-17.
[2] Anil, K. and Ashatha, S. (2012) Review on Hibiscus rosa-sinensis. Int. J. Res. Pharma. Biomed. Sci. 3:534-538.
[3] Ciska, E. and Pathak, D. R. (2004), Glucosinolate derivatives in stored fermentedcabbage. J. Agric. Food Chem. 52 : 7938-7943.
[4] Faurschou, A., Wulf, H. C .(2004).Durability of the sun protection factor provided by dihydroxyacetone. Photodermat. Photoimmuno. And photomed. 20:239-242.
[5] Gablie, B. S. and Deshmukh, S. P. (2010). Synthesis of S-Tetra O-Acetyl Galactopyranosyl Aryldithiocarbamates. Int. J. Chem. Sci. 8:2083-2088...
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| Paper Type | : | Research Paper |
| Title | : | Influence of Environmental Parameters on Lipase Production by Serratia Marcesens |
| Country | : | Nigeria. |
| Authors | : | EJIKE, E.N. || EJIKE, B.U. || ONYEANULA, E.O. |
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: | 10.9790/264X-03052833 |
Abstract: Lipase-producing bacteria were isolated from the soil in Ohuhu Community in Umuahia North Local Government Area of Abia State, The crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture and Rhodamine B dye and their lipolytic activities examined. Among the several bacteria screened, a strain was selected for lipase production as it showed the largest orange fluorescence under UV light, indicating effective production of lipase. Lipase production was carried out using a basal medium containing KH2PO4, Na2SO4, MgSO4.7H2O, Yeast extract, K2HPO4, Peptone, Glucose and Olive oil. Morphological, biochemical and molecular characterization of the isolate identified it as Serratia marcescens. Various physicochemical parameters that were..........
Key words: Lipase, Rhodamine B agar, Serratia marcescens, orange fluorescence, UV light
[1] Adinarayana, K., Bapi, R., Iqbal, Z., Bhavani, D., Jhansi, L. and Ellaiah, P. (2004). Optimization of process parameters for production of lipase in solid-state fermentation by newly isolated Aspergillus species. Journal of Biotech.3:65-69
[2] Akanbi, T.O., Kamarizama, A.L., Abubakar, F., Shekh Abdul Hamid, N. and Radu, S. (2010). Highly thermostable extracellular lipase-producing Bacillus strain isolated from a Malaysian Hotspring and identified using 16S rRNA gene sequencing. International Food Res. Journal. 17:45-53.
[3] Anjana, S., Dipa, B. and Rashmi, P. (2009). Optimization of Physical parameters for lipase production from Arthrobacter sp.BGCC#490. India J. of Biochemistry and Biophysics.46:178-183.
[4] Dheeman, D.S., Frias, J.M. and Henehan, G.T.M. (2010). Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304. J. Ind. Microbiol. Biotechnol. 37, 1–17
[5] Ghosh, P.K, T.K. Saxena, R. Gupta, R.P.Yadar and S. Dvidson(1996). Microbial lipases: Production and Applications. Sci Prog.79: 119-157....
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| Paper Type | : | Research Paper |
| Title | : | Studies on Phytochemical Screening and Antifungal Activity of Tinospora cordifolia |
| Country | : | India. |
| Authors | : | Neetu Thakur |
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: | 10.9790/264X-03053438 |
Abstract: Fruit rot is a very common and destructive disease that causes serious economic loss in production mainly of fruits and vegetables. Environmental friendly plant extracts have shown great potential as an alternative to synthetic fungicides. In the present study, premilinary phytochemical screening of Tinospora cordifolia revealed the presence of carbohydrates, proteins, aromatic amino acids, alkaloids, terpenoids, saponins, tannins, flavanoid s, phenolics, phlobatannins and sterols. The antifungal activity was tested against the two rot fungi i.e. Alternaria alternat a and Fusarium solani. Whole plant extract of T.cordifolia appeared significantly more effective to control the vegetative growth of Alternaria alternata than that of Fusarium solani.
Key words: Phytochemical Analysis, Antifungal Activity, Tinospora cordifolia , Rot fungi
[1]. Droby, S. 2006. Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and nat ural materials. Acta Hortic . 709: 45–51.
[2]. Zhu, S.J. 2006. Non-chemical approaches to decay control in post harvest fruit. In: B. Noureddine , S. Norio (Eds.), Advances in Post harvest Technologies for Horticultural Crops, Trivandrum, India : Research Signpost, pp. 297-313.
[3]. Singh, D., R.R. Sharma. 2007 . Post harvest diseases of fruit and vegetables a nd their management. In:
[4]. D. Prasad, (Ed.), Sustainable Pest Management, and New Delhi, India: Daya Publishing House.
[5]. Ogawa, J.M., E.I. Dehr, G.W. Bird, D.F. Ritchie, V. Kiyoto, J.K. Uyemoto. 1995. Compendium ofStone fruit Diseases, APS Press, USA.
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| Paper Type | : | Research Paper |
| Title | : | Comparative Studies on the Nutritional and Anti – Nutritional Properties of Indigenous Seeds Used As Soup Thickeners in South-East Nigeria |
| Country | : | Nigeria. |
| Authors | : | Ndulaka, J. C. || Ekaiko, M. U || Onuh, Emeka .F || Okoro, Oriaku. A. |
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: | 10.9790/264X-03053944 |
Abstract: The Proximate, minerals, vitamins and phytochemical compositions of six indigenous seeds used as soup thickeners in South East Nigeria were investigated using standard procedures. The soup thickeners which included Mucuna flagelipes (Ukpo), Defarium microcarpum (Ofor), Brachystegia eurycoma (Achi), Citrullus colocynthis (Melon), Afzelia africana (Akparata) and Irvingia gabonensis (Bush mango) was seen to contain various nutritional and anti-nutritional properties such as carbohydrate 43.32% to 59.64%, Ask 2.55- 4.77%, crude fiber 4.05% to 3.00%, Protein, 15.25-16.71%, Fat, 6.10-17.15, Fiber, 4.05- 3.00. The phytochemical included Tannins 67.24-82.1 mg/100g, Alkaloids, 48.71........
Key words: Minerals, proximate, phytochemical, soup thickeners, South East Nigeria
[1]. Abukakar, M. C., Ukwani, A. N. and Shehu, R. A. (2008). Phytochemical Screening and Antibacterial Activity of Tamarindus.
[2]. Akpata, M. I. and Miachi, O. E. (2001). Proximate composition and selected functional properties of Detarium microcarpum. Plant Food for Human Nutrition, 56(4): 297-302.
[3]. AOAC, (1980). Official methods of Analysis. 13th edition Association of Official Analytical Chemists, Washington, D.C.
[4]. Bako, S. P., Bakfur, M. J., John, I. and Bala, E. I. (2005). Ethnomedicinal and Phytochemical Profile of Some Savanah Plant Species in Nigeria. I nternational Journal of Botany, 1(2): 147 – 150.
[5]. Barminas, J. Y., James, M. K. and Abubakar, U. M. (2004). Chemical composition of seeds and oil of Xlopia aethiopica grown in Nigeria. Plant Foods for Human Nutrition (formerly Qualitas planetarium), 53(3): 193-198..
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| Paper Type | : | Research Paper |
| Title | : | IL-16 and XRCC1 gene polymorphism along with hypoalbuminemia correlates with the risk and prognosis of NPC among the ethnic population of northeast region of India |
| Country | : | India. |
| Authors | : | Deepak Singh Lourembam || Thiyam Ramsing Singh || Lisam Shanjukumar Singh |
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: | 10.9790/264X-03054553 |
Abstract: Incidence of Nasopharyngeal cancer (NPC) is high in north-eastern region of India, although it is rare in rest of country. Combined effect of environmental factors, genetic susceptibility and Epstein-Barr virus (EBV) plays an important role in NPC pathogenesis. This study investigates the association of NPC with genetic polymorphisms; XRCC1 and Interleukin 16, viral genetic variant; EBV subtype and latent membrane protein 1 (LMP1), and serum profiles; albumin and immunoglobulin. XRCC1 codon 399 and IL-16 rs11556218 gene polymorphism were determined by PCR-RFLP. PCR assay was used for typing EBV and detecting 30 bp deletion of LMP1 gene. Serum profile was measured by capillary electrophoresis. Statistical analysis was performed by GraphPad Prism. Analysis.......
Key words: EBV, Albumin, Genetic Polymorphism, Biomarker, Nasopharyngeal carcinoma
[1] Chang ET, Adami HO. The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. Oct 2006;15(10):1765-1777.
[2] Forman D, Bray F, Brewster D, et al. Cancer incidence in five continents, Volume X. Lyon, Geneva: International Agency for Research on Cancer; Distributed by WHO Press. World Health Organization. 2014.
[3] Sharma TD, Singh TT, Laishram RS, Sharma LD, Sunita AK, Imchen LT. Nasopharyngeal carcinoma--a clinico-pathological study in a regional cancer centre of northeastern India. Asian Pacific journal of cancer prevention : APJCP. 2011;12(6):1583-1587.
[4] Tsao SW, Yip YL, Tsang CM, et al. Etiological factors of nasopharyngeal carcinoma. Oral oncology. May 2014;50(5):330-338.
[5] Lourembam DS, Singh AR, Sharma TD, Singh TS, Singh TR, Singh LS. Evaluation of Risk Factors for Nasopharyngeal Carcinoma in a High-risk Area of India, the Northeastern Region. Asian Pacific journal of cancer prevention : APJCP. 2015;16(12):4927-4935.
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| Paper Type | : | Research Paper |
| Title | : | Significance of different concentration of plant growth regulators(PGRs) on in vitro multiplication of Dendrobium moschatum(Buch.- Ham.)Sw. using nodular explants |
| Country | : | India. |
| Authors | : | Purnima Saikia || P.K.Borua |
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: | 10.9790/264X-03055457 |
Abstract: Dendrobium moschatum(Buch.-Ham.)Sw.(The Musky Smelling Dendrobium) is an epiphytic orchid of N.E.India which produce graceful, intricately fabricated beautiful flowers of immense floricultural appeal. It is a robust species with erect radiating branches ,bearing sweetly scented flowers with light yellow and purplish tinge on petals and sepals having reddish veins. Like other orchids of this region, this species is also endangered due to large scale denundation of forest areas. So in order to preserve it from further extinction, attempts have been made to conserve and multiply it through in vitro culture of nodular explants. NAA at concentration 1.0 mg/L in MS medium was found to be the most effective for early initiation of bud and multiple shoot induction. Full strength liquid MS medium has been found to produce the best result for nodular cultured explants.
Key words: in vitro technique, D.moschatum, MS medium, nodular segments, NAA, IAA, BAP, liquid MS medium.
[1] Adritti, J. Michaud, J.D. and Olivia, A.P. (1981). Seed germination of North American Orchids. Native California and related species of Calypso ,Epipactis , Goodyera, Piperia, Platanthera, Bot. Gaz., 142: 442-453.
[2] Arora,Y.K. and Mukherjee,A. (1983). Ornamental orchids of N.E.I ndia, Technical Bulletin No. 5,ICAR Complex of NEH Region, Shillong, Meghalaya.
[3] Chadha, K.L. (1994). Orchid floriculture in India. J. Orchid Soc. India., 8(1-2), :1-4.
[4] Chennaveeriah, M.S. and Patil, S.J. (1975). Morphogenesis in seed culture of Spathoglottis. Curr.Sci., 44:68.
[5] Gobaerts,R.(2013). Kew World Checklist of Selected Plant Families,,Dendrobium moschatum(Buch.-Ham.)SW.1805. 58450
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| Paper Type | : | Research Paper |
| Title | : | Mitigating the Effect of Terminalia chebula Retz., Terminalia bellirica (Gaertn) Roxb. and Phyllanthus emblica L. on Imidacloprid Insecticide Induced Toxemia in Albino Wistar Rats |
| Country | : | India. |
| Authors | : | Dr.V.Eugin Amala || Dr.M.Jeyaraj || M. Meldintha Mary |
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: | 10.9790/264X-03055862 |
Abstract: Indiscriminate usage of pesticides in agriculture is leading to contamination of environment and natural resources thereby producing an adverse impact on animal and human health. Administration of Imidacloprid through various routes in animals produces marked alterations in serum biochemical and haematological parameters. Healthy adult female rats (Rattus norvegicus) were administered IMI at the dose of 40mg/kg body weight, 80mg/kg body weight orally. Both doses were given individually to investigate the IMI toxicity and its reversal by Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and Triphala (1:1:1). The results revealed that 28 days of exposure insignificant haematological changes in comparison to the control group. This study exhibited that sub-acute oral administration of Imidacloprid at this particular dose of 40mg/kg, 80mg/kg body weight causes mild toxic effect on various haematological parameters in rats.
Key words: Mitigating, Toxemia, Imidacloprid
[1]. Venkatesan S., Pugazhendy K., Meenambal M., Sangeetha D., Asantharaja C.V., Jayachandren K., Prabakaran S. Protective role of Spirulina on the variation of haematological parameter induced by herbicide Atrazine in the fresh water fish Cyprinus carpio (Linn). Int. J. Pharm. Biol. Arch. 3, 2012, 249-254.
[2]. Guyton A.C., Hall J.E . Text book of medical physiology, 10th edition, (WB Saunder's Company, Philadelphia PA,1991).
[3]. Talas Z.S., Gulhan M.F . Effects of various propolis concentrations on biochemical and haematological parameters of rainbow trout (Oncorhynchu mykiss). Ecotoxicology and Environmental safety, 72, 2009, 1994-1998.
[4]. Suvetha L., Ramesh M., Saravanan M. Influence of Cypermethrin toxicity on ionic regulations and gill Na+/K+ ATPase activity of fresh water teleost fish Cyprinus carpio. Environ. Toxicol. Pharmacol. 29(1),2010, 44-49.
[5]. Shahi J., Singh A. Effect of bioactive compounds extracted from Euphorbious plants on haematological and biochemical parameters of Channa punctatus Rev. Ins Med Trop Sao Paulo. 53,2001,259-263.
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| Paper Type | : | Research Paper |
| Title | : | Effect of Light Intensity and Orientation on In-Vitro Regeneration of Cassava Plantlets |
| Country | : | Nigeria |
| Authors | : | Ihuoma C. Okwuonu || Gladys N. Nsofor || Carol I. Ihezie || George Okogbue || Chiedozie N. Egesi |
|
: | 10.9790/264X-03056369 |
Abstract: Replacement of white fluorescent tube (FL) with compact fluorescent light (CFL) as an alternative source of light for tissue culture in developing countries like Nigeria was evaluated. The effect of different CFL bulbs with photon flux densities (PFD) of 23, 41, 51 and 184 μMol m-2s-1arranged in horizontal, angular and vertical orientations on plant height, leaf node formation, root proliferation and plantlets regeneration were investigated. All four factors were differently affected by light intensity and orientation. Plantlet regeneration was positively impacted by light intensity of 51 μMol m-2s-1and by angular orientation, plant height and root proliferation were optimum at light intensity of 184 μMol m-2s-1and vertical orientation respectively, while differences in light intensities and orientation had no significant impact on leaf node formation. Light intensities of 51 μMol m-2s-1 and angular orientation were adopted as optimal for all four factors considered.
Key words: Tissue culture, Light intensity, Light orientation, Photon flux density, plantlet regeneration
[1]. Bello, J. J. B., Sato, J. A. P., Bello, J. B., & Sato, J. A. P. (Ed.). World TM s largest Science, Technology & Medicine Open Access book publisher Light ‐ Emitting Diodes : Progress in Plant Micropropagation Light ‐ Emitting Diodes : Progress in Plant Micropropagation.
[2]. S. Dutta Gupta, andB. Jatothu, Fundamentals and applications of lightemitting diodes (LEDs) in in vitro plant growth and morphogenesis, Plant Biotechnol. Rep, 2013, 7, 211–220. doi: 10.1007/s11816-013-0277-0
[3]. C. Li, Z. Xu, R. Dong, S. Chang, and L. Wang, An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue , Green , Red LED Light , and White Fluorescent Light,(2017). 8(January).http://doi.org/10.3389/fpls.2017.00078
[4]. M.C. WU, C.Y. HOU, C.M. JIANG, Y.T. WANG, and C.Y. WANG, A novel approach of LED light radiation improves the antioxidant activity of pea seedlings, Food Chemistry, Cambridge, n. 101, p. 1753-1758, 2007.
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| Paper Type | : | Research Paper |
| Title | : | Serological Cross-Reactivity of Alphaviruses To Chikungunya Specific Antibodies And Its Relationship To Their Structural proteins |
| Country | : | Kenya |
| Authors | : | Caroline Wasonga |
|
: | 10.9790/264X-03057076 |
Abstract: Alphaviruses are small, enveloped viruses containing a single-stranded, positive-sense RNA genome and belongs to the family Togaviridae. Most of these viruses are transmitted by mosquitoes and infect a variety of mammals and birds.The purpose ofthis study wasto assess the reactivity of selected alphaviruses, namelyO`nyong`nyong virus (ONNV), Ndumu virus (NDUV), Semliki forest virus (SFV) and Sindbis virus (SINV) to an in-house antigen detection Enzyme Linked Immunosorbent Assay (ELISA) developed from Chikungunya virus (CHIKV) specific antibodies. Secondly, the cross-reactivity of these alphaviruses andtheir genetic characteristics when compared to CHIKV was also to.............
Key words: Alphaviruses, chikungunya virus, cross-reaction, phylogenetic analysis
[1]. G. Borgherini, P. Poubeau, A. Jossaume, A. Gouix, L. Cotte, A. Michault, C. Arvin-Berod, and F. Paganin, Persistent arthralgia associated with chikungunya virus: a study of 88 adult patients on reunion island. Clinical Infectious Diseases, 47, 2008, 469–475.
[2]. H. Shore, O'nyongnyong fever: an epidemic virus disease in East Africa III. Some clinical and epidemiological observations in the Northern Province ofUganda, Transactions of theRoyal Society ofTropical Medicine andHygiene,55, 1961, 361–373.
[3]. C. C. Mathiot, G. Grimaud, P. Garry, J. C. Bouquet, A. Mada, A.M. Daguisy and A.J.Georges, "An outbreak of human Semliki forest virus infections in Central African Republic,"American Journal of Tropical Medicine and Hygiene, 42 (4), 1990, 386–393.
[4]. N. K. Blackburn,T. G. Besselaar, G. Gibson, Antigenic relationship between chikungunya virus strains and o`nyong`nyong virus using monoclonal antibodies, Research in Virology, 146, 1995, 69–73
[5]. A.H. Khan, K. Morita, M.C. Parquet, F. Hasebe, E.G.Mathenge, and A. Igarashi,Complete nucleotide sequence of Chikungunya virus and evidence for an internal polyadenylation site,Journal of General Virology, 83, 2002, 3075-3084.
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| Paper Type | : | Research Paper |
| Title | : | Developing Preliminary In-Vitro Disease Assay for Cassava Resistant to Bacterial Blight Disease |
| Country | : | Nigeria |
| Authors | : | Ihuoma C. Okwuonu || Joseph T. Onyeka || ChiedozieN. Egesi |
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: | 10.9790/264X-03057782 |
Abstract: In-vitro disease assay was developed as a preliminary tool for screening cassava resistance to Xanthomonasaxonopodispv.manihotis (Xam), the causative agent of cassava bacterial blight disease. Detached petiole-borne leaf was cultured in cassava rooting medium and inoculated with 0.1 μl of bacterial suspension(Xam transformed with pLux)at OD6000.5. Inoculated plantlet were incubated at 30ºC and monitored daily for bacterial growth via bioluminescence imaging. Root and shoot induction was observed 7 and 14 days after inoculation respectively. Bioluminescence imaging was scored on the scale of 0-3 with scores of 0, 1, 2 and 3 representing absence of growth,...........
Key words: Cassava, in-vitro disease assay, cassava bacterial blight, bioluminescence, Xanthomonasaxonopodis
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