Version-1 (July-August 2018)
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Abstract: Aim The present study was aimed to assess the liver dysfunction among diabetic patients attending the rural teaching hospital. Objectives: To study the activity of serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), Total bilirubin (TB), and serum alkaline phosphatase (ALP) in T2DM patients and compare it with that of standard healthy controls. Materials and Methods: The present study conducted at MNR medical college and hospital, Sangareddy, India. A total of 150 study subjects who are attending the diabetic clinic are involved in this study during the period from February 2018 to May 2018. An institutional ethical committee approved the present study. A total of 100 type 2 diabetic patients...........
Key words: Diabetes mellitus, Liver function tests, Alanine aminotransferase, Aspartate aminotransferase, Alkaline phosphatase, Total bilirubin..
[1]. World Health Organization Consultation. Definition, Diagnosis and Classification of Diabetes Mellitus and its Complications, Part 1: Diagnosis and classification of Diabetes Mellitus. Report of a WHO Consultation. Geneva: World Health Organization, 1999.
[2]. Kiran Kumar Akka, Pampareddy B. Kollur, S.M. Awanti, Nagababu Pyadala. Evaluation of thyroid profile among type 2 diabetic patients attending to Basaveswara Teaching and General Hospital, Kalaburagi, Karnataka. IAIM, 2017; 4(6): 181- 186.
[3]. Sarala Devi Tenepalli, Nagababu Pyadala. Assessment of Oxidative Stress among Type 2 Diabetes Mellitus Patients Attending in A Rural Teaching Hospital, Sangareddy. IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB), 2016; 2(5): 24-27.
[4]. Nagababu Pyadala, Ravindra Reddy Bobbiti, Ragalikhith Kesamneni, Rajaneesh Borugadda, Ravi Kumar, B. N, R. Vijayaraghavan, Rathnagiri Polavarapu. Association of Glycosylated hemoglobin and Lipid profile levels among Type 2 diabetic patients in Sangareddy. Research Journal of Pharmaceutical, biological and chemical Sciences, 7(5): 2849.
[5]. Gavin N. Levinthal and Anthony S. Tavill: Liver Disease and Diabetes Mellitus. Clinical Diabetes 1999, 17 (2):85..
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Abstract: Soap bars are ideally considered to be free of organisms based on their high alkalinity & salt levels and thus considered not as candidate for spoilage, but repeated incidences of off colours/spots reported over the past has lead to increase incidences of rejection. Thus, in this study 20 unbranded soap bars available in local markets of Mumbai suburbs were screened for the presence of moderately halophilic gram positive cocci with special reference to the lesser known Nesterenkonia species, an alkalophilic organism. Eight different media known to support alkalophilic and halophilic organisms used to assess the microbiological load, revealed growth of organisms within the range of 103 to 105cfu/g. 151 isolates were picked up as representative of Operational Taxonomical Unit (OTU) of which 65 isolates were identified to be gram positive cocci on the basis of their cultural, morphological and biochemical characters. Comparison of 16S rRNA gene sequences showed that 4 isolates were phylogenetically closely related to Nesterenkonia species. Thus, survival of an extremophile organism like Nesterenkonia within the soap bars require strict imposition of microbiological...........
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[3]. Buffet-Bataillon S., et al. (2009) Outbreak of Serratia marcescens in a neonatal intensive care unit: contaminated unmedicated liquid soap and risk factors. J. Hosp. Infect. 72:17–22.
[4]. Carrie A. Zapka, Esther J. Campbell, Sheri L. Maxwell, Charles P. Gerba, Michael J. Dolan, James W. Arbogast, & David R. Macinga (2011) Bacterial Hand Contamination and Transfer after Use of Contaminated Bulk-Soap-Refillable Dispensers. Appl Environ Microbiol 77(9): 2898–2904.
[5]. Collins MD, PA Lawson, M Labrenz, BJ Tindall, N Weiss and P Hirsch, (2002) Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho lake, East Antartica and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol, 52: 1145 -1150..
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Abstract: Worldwide emergence of new resistance mechanisms in constantly increasing infections of Multidrug Resistance (MDR) bacteria (such as Methicillin resistant Staphylococcus aureus (MRSA) and Salmonella) is generating a major problem in treatment or prevention of infection against these pathogens. Development of resistance reduces the effectiveness of antibiotics against them. Antibiotics are costly and also harm useful microbes of host body, thus there is a need to adopt drug-independent remedial strategies to defeat pathogens. Phage therapy has therefore been considered as one of the strongest alternatives to antibiotics. Host specific Bacteriophages naturally and specifically kill their host without disturbing the normal microbes of host organism also provides cost effective treatment..........
Key words: Multidrug resistance, phage therapy, Methicillin resistance Staphylococcus aureus (MRSA), Salmonella typhi, Bacteriophages, sewage, pathogens, plaque assay.
[1]. Arlet G, BarrettTJ, ButayeP, CloeckaertA, MulveyMR, and White DG (2006) Salmonella resistant to extended-spectrum cephalosporins: prevalence and epidemiology. Microbes Infect. 8:1945–1954.
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Abstract: Introduction: External Quality Assessment Scheme (EQAS) involves evaluation of a number of laboratories by an outside agency on the performance of a number of laboratories based on their analytical performance of tests on samples supplied by the external agency. EQAS performance has been shown to reflect the quality of patient specimen testing in a clinical laboratory. Aim : To evaluate our performance in terms of the performance indicators(SDI, VIS) used by the EQAS body. Materials and Methods: EQAS results were evaluated since our participation in the EQAS clinical chemistry monthly programme from BIO- RAD laboratories for the period July 2016 to July 2018 (cycle 15and cycle 16 each cantaining 12 samples) in terms of statistic.........
Key words: EQAS, VIS, SDI/Z-Score
[1]. Westgard JO. Internal quality control: planning and implementation strategies. Ann Clin Biochem. 2003;40:593-611.
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[3]. Yadav R, Bhartiya JP,Verma SK, Nandkeoliar MK. External quality assessment scheme(EQAS) our experience as a participating laboratory. IJRRMS 2013;3(4)
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[5]. Miller WG, Myers GL, Rej R. Why commutability matters. Clin Chem. 2006;52:553-54..
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Abstract: Moringa oliefera is one of the plants that is scientifically proven to contain secondary metabolites of flavonoids, alkaloids, phenols that can inhibit bacterial activity. Extraction of bioactive compounds from plants is considered inefficient because it requires a large biomass. The effective method is by utilizing endophytic bacteria associated with the plant. The aim of this research were to know the ability of endophytic bacterial isolate of bark of moringa plants in inhibiting the growth of gram-positive and gram-negative bacteria, and to characterize the biochemical and morphologycal properties of the isolated endophytic bacteria, and to determine species of endophytic bacterial isolate based on 16S rRNA. The stage of this research were endophytic bacterial isolation, antibacterial........
Key words: antibacterial activity, endophytic bacteria, stem of moringa plant, 16S rRNA
[1]. Krisnadi, A.D. 2015. Moringa Super Nutrisi. www.moringaina.com
[2]. Bukar, A., Uba, A., Oyeyi, T.I. 2010. Antimicrobial Profil of Moringa oleifera Lam. Extracts Against Some Food-Borne Microorganism. Bajero Journalof Pure and Applied Sciences. Vol. 3(1) : 43-48.
[3]. Fahey, J. W. 2005. Moringa oleifera: A Review of the Medical Evidence for its Nutritional, Therapeutic, and Prophylactic Properties. Part I. (http://www.malunggay-propagation.com/Jed_Fahey_text_GB.pdf)
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[5]. Ikalinus,R., Sri Kayati, W., Ni Luh Eka S.,2015. Skrining Fitokimia Ekstrak Etanol Kulit Batang Moringa (Moringa oleifera). Indonesia Medicus Veterinus 4(1) : 71-79..
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Abstract: Free radical stress leads to tissue injury and progression of disease conditions such as arthritis, hemorrhagic shock, atherosclerosis, diabetes, hepatic injury, aging and ischemia, reperfusion injury of many tissues, gastritis, tumor promotion, neurodegenerative diseases and carcinogenesis. Safer antioxidants suitable for long term use are needed to prevent or stop the progression of free radical mediated disorders. Many plants possess antioxidant ingredients that provide efficacy by additive or synergistic activities. Madhuca longifolia L leaves are used as strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. Antioxidant activity of the......
Key words: Madhuca longifolia L, antioxidant, Triterpene, GSH and LPO
[1]. Allen LH. Mechanisms of pathogenesis: Evasion of killing by polymorph nuclear leukocytes. Microbes Infect. 2003; 5: 1329-1335.
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Abstract: Cassava (Manihot esculenta) is the third biggest source of carbohydrates after rice and maize, feeding almost a billion people daily in the tropics. Recently it has been proposed as one of the more ideal biomass producers for biofuel production due to its high biomass yield and low input needs. A robust reproducible genetic transformation protocol is needed for germplasm improvement or functional genomics studies in cassava. In the present study we report, for the first time the establishment of a reproducible Agrobacterium-mediated transformation protocol for cassava landraces Mkombozi and Albert preferred by farmers in the coastal region of east Africa. Friable......
Key words: Cassava, Friable embryogenic calli, genetic transformation, transgenic plants
[1]. S. Barampuram, J. Zhang, Plant chromosome engineering, Recent advances in plant transformation. 701 (2011) 1-35.
[2]. G. Beyene, R. Deepika, H. Wagaba, T. Moll, T. Alicai, D. Miano, J. Carrington, N. Taylor, Loss of CMD2-mediated resistance to cassava mosaic disease in plants regenerated through somatic embryogenesis, Molecular Plant Pathology. 12 (2015) 1-16
[3]. E.Bull, A. Owiti, M. Niklaus, R. Beeching, W. Gruissem, H. Vanderschuren, Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava, Nature Protocols. 12 (2009) 1845–1854.A.Burns, R. Gleadow, J. Cliff, A. Zacarias, T. Cavagnaro, Cassava The Drought, War and Famine Crop in a Changing World. Sustainability 2 (2010) 3572-3607
[4]. B.Chetty, C. Rossin, W. Gruissem, H. Vanderschuren, M. Rey, Empowering biotechnology in southern Africa: establishment of a robust transformation platform for the production of transgenic industrypreferred cassava, New Biotechnology Journal. 30 (2012) 136-143.
[5]. FAO. Cassava market summary, Food Outlook Global Market Analysis. (2011) 39–43...
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Abstract: Breast cancer is the most common type of cancer causing death in women globally. It is highly metastatic and can spread to various parts of body. There are many studies regarding role of cytokines in development of cancer. The cytokine NF-κB plays a major role in development and proliferation of cancer cells. Along with it TNF-alpha plays a dual role. According to its presence it can be tumour promoter and on the other hand it can be a cancer killer. The property of TNF in inducing cancer cell death renders it a potential cancer therapeutic, although much work is needed to reduce its toxicity for systematic TNF administration. Current treatments that are available for cancer are costly and have many side-effects. The main aim of this research was to determine in-silicoimmunomodulatory......
Key words: Breast cancer, Immunomodulaion,Nuclear factor - kappaB (NF-κB),Ocimumtenuiflorum L. (holy basil),Tumournecrosis factor – alpha (TNF-alpha), Zingiberofficinale R. (ginger),
[1]. Silverstein MJ. The University of Southern California/Van Nuys prognostic index for ductal carcinoma in situ of the breast. The American Journal of Surgery . 2003; 186(4):337-343.
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Abstract: This study consisted initially in formulating 3 feeds (A1, A2 and A3) intended to feed poultry from local products available, at a lower cost and not appreciated for human consumption. Beside these 3 formulated feeds, there was an AT trade feed that was used as a control feed. Then these foods were analyzed. The results revealed that the formulated foods are true sources of minerals (macro-elements such as Calcium, Potassium, magnesium and phosphorus and micro-elements such as iron, manganese, Sodium, copper and Zinc) and vitamins (A, B1, B2 and C). Also contain anti-nutritional substances (Oxalates, tannins, and phytate). In our study, the Phytate/Ca and phytate/Fer ratios are respectively below 2.5 and above 0.4. The major foodstuffs that have been taken into account in the formulation of these foods are maize as a carbohydrate source, pulp and seed(Parkiabiglobosa)grains as a vegetable protein......
Key words: Parkiabiglobosa, Flour, seed, snail, corn
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Abstract: The presentinvestigation is one of the first works on micropropagation of the seedling strain (Shabah) dry date palm (Phoenix dactylifera L.) grown at Aswan Governorate. Explants (shoot tips and leaf primordia) were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of casein hydrolysate (CH). The highest swelling and callus initiation percentage were achieved at 2 g/L of CH. Results indicated that the shoot tips gave the highest swelling and callus initiation percentage. The percentage of embryogenic callus formation obtained from shoot tip and leaf primordia explants reached the highest value, when cultured on MS medium with 3 mg/L 6-benzyl adenine (BA) + 10 mg/Lnaphthalene acetic acid (NAA) + 10 mg/L 2,4-dichlorphenoxy acetic acid (2,4-D), compared with other tested treatments. In addition, the same medium recorded the highest fresh weight of.....
Key words: In vitro,embryo formation, soot tip, leaf primordial, casein hydrolysate, salicylic acid, polyethylene glycol
[1]. Abdel-Raheem, A.T., A.R. Ragab, Z.A. Kasem, F.D. Omar and A.M. Samera (2007). In vitro selection for tomato plants for drought tolerance via callus culture under polyethylene glycol (PEG) and mannitol treatments. African Crop Science Conference Proceedings, 8: 2027-2032.
[2]. Al-Khateeb, A.A. and H. Ghazzawy (2015). Influence of polyethylene glycol and gibberellin on somatic embryogenesis of date palm (Phoenix dactylifera L.) cv Sukkary. Journal of Global Biosciences, 6: 2446-2451.
[3]. Al-Khayri, J.M. (2005). Date Palm Phoenix dactylifera L. In: Protocols of Somatic Embryogenesis in Woody Plants. Jain, S.M. and P.K. Gupta (Eds). Springer, Berlin, p. 309-318.
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[5]. Al-Khayri, J.M. and P.M. Naik (2017). Date palm micropropagation: Advances and applications. Ciencie Agrotecnologia, 41: 347-358...
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Abstract: Aim: Asthma is a clinical condition which causes constriction in airway with shortness of breath, chest tightness, cough and wheeze. It can be mild, moderate and severe. Vitamin D is obtained directly from sunlight and through the diet. It is a fat-soluble natural nutrient which is a modulator of calcium absorption and bone health. It also plays an important role in immune regulation and in respiratory infections. Materials & Methods: This study was conducted in the department of Biochemistry, People's College of Medical Science & Research Centre Bhopal (M.P.). For the study purpose 100 cases and 100 controls were selected randomly and perform Vitamin D test by CLIA method (kit method) in the laboratory. This study was based on comparison of serum vitamin D level of cases and controls. Compare serum vitamin D level according to the age groups of patients and according......
Key words: Vitamin D, Asthma, Immune system, infection
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[2]. The Global Asthma Report 2014. Auckland, New Zealand:Global Asthma Network, 2014. Available from: http://www.globalasthmareport.org/resources/Global_Asthma_Report_2014.pdf, accessed on February 16, 2015.
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Paper Type | : | Research Paper |
Title | : | Polygraph Test and Its Relevancy in Criminal Justice System |
Country | : | India |
Authors | : | Dr SeemaYadav || Dr SandhyaVerma |
: | 10.9790/264X-0404017682 |
Abstract: Law is dynamic and not static and therefore, as society evolves law has to keep in consonance with the changing social order. Law is the instrument of societal change and the judiciary has the responsibility of interpreting the law for the greater good. Therefore, it is clear that the judicial mind must stay in touch and keep in step with the advancement of humanity. To combat organized crime, its detection, investigation and prevention method have to be employed synchronously. If the criminal use new technology in committing the crimes, the enforcement agencies need to use the new techniques in solving such crime. If the enforcement agencies do not use these technologies for solving such complicated crimes, it would be very difficult to detect the perpetrators of such crime.......
[1]. Donald Krapohl and Pamela Shaw, Fundamentals of Polygraph Practice 2(Elsevier, UK, 2015).
[2]. Ibid.
[3]. Dr. Ganapati M. Tarase, Dr. Prakash D. Haveripeth& Dr. M. S. Ramadurg, "Scientific and Legal Procedure of Polygraph Test" 2(1) J.Bio.Innov5(2013).
[4]. "Constitutional validity of and evidentiary validity of scientific test"49:4 Journal of Indian Law Institute 529 (2007).
[5]. Supra 2 at 7.
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Paper Type | : | Research Paper |
Title | : | Near Field Optical Properties of Aggregated DNA particles |
Country | : | India |
Authors | : | Rakesh K. Rai || Snigdha Lal |
: | 10.9790/264X-04048387 |
Abstract: Surface enhanced Raman scattering (SERS) is observed in highly conducting materials such as gold and silver. Near field calculations of light scattering is exetended to bio-molecules and bio-molecules on gold nanometer plate. Near field optics of scattered light from DNA aggregate in spherical shape is theoretically considered. The spherical DNA molecules aggregate is placed on a gold nanoplate to ascertain its near field optics. Two different type materials- irregular shaped dielectric materials on gold nanoplates are considered in near electric field calculations. These surface enhanced scattering are calculated for non-spherical shapes by approximate methods
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