ABSTRACT: Four, one-stage leach bed reactors (LBRs) were studied for application of rumen cultures as an inoculum source for enhancing hydrolysis during dry anaerobic mono-digestion (AD) of grass silage at 35oC.Two LBRs were employed to test the effect of rumen culturewith (25% rumen culture + 75% digested cow manure) and without combining the digested cow manure (i.e., 100% rumen culture). Another Two LBRs were employed to compare the hydrolytic degradation efficiencies of NaOH pre-treated and untreated grass silage using digested cow manure (100%) as inoculum. Results showed that, application of a combination of rumen inoculum @ 25 % of rumen culture + 75 % digested cow manure or 100 % rumen culture as an inoculum source, enhanced hydrolysis of grass silage by 10 % than in...........
Keywords: Leach bed reactor, hydrolysis, grass silage, rumen fluid, methane, inoculum.
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