Version-1 (Sep–Oct 2016)
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| Paper Type | : | Research Paper |
| Title | : | IGF1 Gene As A Target For Genetic Diversity Among Indian Poultry Birds |
| Country | : | India |
| Authors | : | Ahmed Kareem Alatafi || kondapalli kasturi |
Abstract: The genes of Growth Hormone (GH) and Insulin-like Growth Factor 1 (IGF-1) are responsible for growth in animals as they play a key role in the regulation of their growth and development. The native breeds exhibit great Genetic polymorphism and there is a major concern for the necessity of preserving genetic resources. It is very important and significant to characterize and differentiate the genetically indigenous breeds.The present work deals with applying the RFLP.............
Key words: IGF1, RFLP, genetic diversity, chickens
[1] 1.Aminafshar Mehdi* and Fathi Ali Reza Single nucleotide Polymorphisms in intron 1 of growth hormone gene and it‟s association with economic important traits in Iranian Fars native fowl Scholars Research Library Annals of Biological Research, 2012, 3 (8):4028-4032 (http://scholarsresearchlibrary.com/archive.html)
[2] 2.http://agritech.tnau.ac.in/animal_husbandry/ani_chik_breeds%20of%20chicken.html
[3] 3. Tixier-Boichard, M., Bordas, A. & Rognon, X. 2008. Characterization and monitoring of poultry genetic resources. Proceedings of the 23rd World‟s Poultry Congress, Brisbane, Australia, July 2008. CD-ROM.
[4] 4. Robert Pym, Poultry genetics and breeding in developing countries Genetic diversity and conservation of genetic resources; Food and Agriculture Organization of the United Nations Poultry Development Review
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| Paper Type | : | Research Paper |
| Title | : | Computation of Melting Temperatures (Tm) of oligonucleotides for high throughput PCR using MS-Excel |
| Country | : | INDIA |
| Authors | : | Lavanya Kumari.P || Amaravathi.Y |
Abstract: DNA based molecular markers have enormous potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection. Most of the molecular markers are individually amplified by oligonucleotide primers using PCR. The efficiency of PCR depends on primer annealing temperature ( Ta) which optimize amplification and minimize non-specific binding of primers to the template. In contrast to the majority of the software programs available to calculate melting temperature (Tm) of primers/oligonucleotides which enables us to get Tm for single sequence/ input at a time..............
Key words: PCR, DNA based markers, Marker Assisted Selection (MAS,) Melting Temperature (Tm), Annealing Temperature (Ta), MS-Excel
[1] Breslauer, K.J., F. Ronald, H. Blocker, and L.A. Marky. 1986. Predicting DNA duplex stability from the base sequence. Proc. Natl. Acad. Sci. 83: 3746-3750.
[2] Freier, S.M., R. Kierzek, J.A. Jaeger, N. Sugimoto, M.H. Caruthers, T. Neilson, and D.H. Turner.1986. Improved free-energy parameters for predictions of RNA duplex stability. Proc. Natl.Acad. Sci. 83: 9373-9377.
[3] Howley,P.M., Israel,M.F., Law,M-F., and Martin,M.A. (1979) J BiolChem 254:4876-4883.
[4] Marmur,J., and Doty,P. (1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature, J Mol Biol 5:109-118.
[5] Nakano et al, (1999) Proc. Nuclec Acids Res. 27:2957-65.
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| Paper Type | : | Research Paper |
| Title | : | Is Lipid Accumulation Product (LAP), a better obesity index in diagnosing cardiovascular disease |
| Country | : | INDIA |
| Authors | : | Dr Nirmitha Dev M |
Abstract: Background: It has been shown that low grade inflammatory state is associated with cardiovascular risk. High sensitive C-reactive protein (hs-CRP), a nonspecific inflammatory marker has been shown to be increased in metabolic syndrome a risk state for the development of cardiovascular disease. Obesity is a predisposing condition to metabolic syndrome. Therefore, this study was intended to compare the indices available to measure obesity such as BMI and "lipid accumulation product" (LAP) with respect to risk for development of cardiovascular..............
Key words: Obesity, Lipid Accumulation Product (LAP), High sensitive C-reactive protein (hs-CRP), Body Mass Index (BMI).
[1]. World Health Organization Controlling the global obesity epidemic. Available at: http:// www. who. int/nutrition/ topics/ obesity/en/index.html
[2]. Trayhurn, P., Wood, I.S., (2004), "Adipokines: inflammation and the pleiotropic role of white adipose tissue", Br J Nutr 92:347–355
[3]. Weisberg, S.P., McCann, Desai, M., et al.(2003), "Obesity is associated with macrophage accumulation in adipose tissue", J. Clin. Invest, 112:1796-1808
[4]. Li, J-J., Fang, C.H., (2004), "C -reactive protein is not only an inflammatory marker but also a direct cause of Cardiovascular diseases", Medical Hypothesis, 62:499-506
[5]. Sartipy, P., and Loskutoff, D.J. (2003), "Monocyte chemoattractant protein-1 in Obesity and insulin resistance", Proc. Natl. Acad. Sci. U. S. A, 100:7265-7270.
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| Paper Type | : | Research Paper |
| Title | : | Association between Microalbuminuria and HbA1C among known Type 2 Diabetic patients in puducherry population-A comparative study |
| Country | : | India. |
| Authors | : | Muraliswaran P || Aparna G || Kanagavalli P || Srikanth S |
Abstract: Diabetes mellitus is the commonest metabolic disorder and has a high prevalence in India. Diabetic nephropathy is characterized by proteinuria and is the leading cause of end stage renal disease. The prevalence of microalbuminuria predicts progression to diabetic nephropathy. This cross sectional study was carried out in the diabetic clinic at SVMCH and RC. Sixty known Type 2 diabetic patients with age 30-70 years of both genders were included in the study. Fasting, postprandial venous blood and morning urine sample was collected for analysis of blood glucose, HbA1C and microalbuminuria levels. Statistical analysis was done using SPSS version 17.0. Pearson correlation and Chi square test was applied to observe association of microalbuminuria with different parameters.........
Key words: Type 2 Diabetes Mellitus, Microalbuminuria, HbA1C, Risk Factor, Nephropathy.
[1]. Defronzo RA. Diabetes Rev 1997; 5:177-269.
[2]. King H, Aubert RE, Herman WH. Diabetes Care 1998; 21: 1414-31.
[3]. Nathan DM, Turgeon H, Regan S. Diabetologia 2007; 50, 2239-2244.
[4]. www.care.diabetes journals/contents/27/Suppl_1/s79-s83.
[5]. De Cosmo S, Piras GP et al. Diabetologia 1997; 40: 1191-6..
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| Paper Type | : | Research Paper |
| Title | : | Electrolyte disturbances in elderly population of coastal Karnataka |
| Country | : | INDIA |
| Authors | : | Dr. Usha Sachidananda Adiga |
Abstract: Introduction: Various anatomical and physiological changes have been reported in renal system with advancing age. These changes might lead to electrolyte disturbances in elderly even in the absence of kidney diseases, which are responsible for high morbidity and mortality . Electrolyte disorders in elderly are least explored in India. So we aim to conduct a retrospective comparison of serum electrolytes in elderly and young adults as well as to study the correlation between age and serum electrolytes.............
Key words: Elderly population, serum electrolytes, imbalances.
[1]. Allison SP, Lobo DN. 2004.Fluid and electrolytes in the elderly. CurrOpinClinNutrMetab Care 7:27e33.
[2]. Gaspar PM.1999. Water intake of nursing home residents. J GerontolNurs25:23e9.
[3]. Foroni M, Salvioli G, Rielli R, Goldoni CA, Orlandi G, ZauliSajani S, et al. 2007.A retrospective study on heat-related mortality in an elderly population during the 2003 heat wave in Modena, Italy: the Argento Project. J Gerontol A BiolSci Med Sci62:647e51.
[4]. Hawkins RC. 2003.Age and gender as risk factors for hyponatremia and hypernatremia. ClinicaChimicaActa377:169–172.
[5]. Chumlea WC, Guo SS, Zeller CM, Reo NV, Siervogel RM.1999. Total body water data for white adults18 to 64 years of age: the FELS Longitudinal study. Kidney Int56:244–252.
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| Paper Type | : | Research Paper |
| Title | : | An Impact Of Diet On Oxidative Stress In Essential Hypertension |
| Country | : | INDIA |
| Authors | : | Dr. P. Jayakala M. B. B. S., M. D |
Abstract: Hypertension (HTN) is a major modifiable risk factor for Cardio Vascular Disease. Hypertension doubles the risk of cardiovascular diseases, including coronary heart disease (CHD), congestive cardiac failure (CCF), ischemic and hemorrhagic stroke, renal failure, and peripheral arterial disease. In India, the incidence of HTN occurs between 3rd and 6th decades of life. Also HTN leads to increased oxidative stress, in turn leads to atherosclerosis and dyslipidemia. Among the hypertensives, the oxidative stress is more marked in the non vegetarian groups. In this study, oxidative stress parameters serum uric acid and malon di aldehyde were estimated and was compared in both dietary groups. Routine investigations and the serum uric acid was estimated by standard kit method..............
Key words: HTN (Hypertension),MDA(Malan di aldehyde),CHD(coronary heart disease),CCF(congestive cardiac failure), LPO(lipid peroxidation).TC(Total cholesterol), TGL(Triglycerides.
[1]. Millar-Craig M et al. Circadian variation of blood pressure. Lancet 1978;1:795–797
[2]. Mancia G et al. Blood pressure and heart rate variabilities in normotensive and hypertensive human beings.Circ Res 1983;53:96–104
[3]. Kearney PM et al. Global burden of hypertension: analysis of worldwide data. Lancet 2005 Jan 15-21;365(9455): 217-23.
[4]. Feng J. He and Graham A. MacGregor*Blood pressure is the most important cause of death and disability in the world, Blood Pressure Unit, Cardiac and Vascular Sciences, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK European Heart Journal Supplements (2007) 9 (Supplement B), B23–B28
[5]. Puig JG, Ruilope LM. Uric acid as a cardiovascular risk factor in arterial hypertension. Journal of Hypertension 1999;869-872..
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| Paper Type | : | Research Paper |
| Title | : | Mutagenic Action of Sodium Azide on Phytic Acid and Cooking Time in the First Mutant Generation M1 of Mangu Beans (Phaseolus vulgaris L.) |
| Country | : | Nigeria |
| Authors | : | Liamngee, Mlahaga Sorishima || Shaahu David Terhemba || Okwoli Aboje Agaba || Ugbaa MacSamuel Sesugh |
Abstract: Phytic acid is a potent anti-nutrient;it reduces the bioavailability of certain nutrients and inhibits enzyme activity. This can impact negatively on metabolic pathways that require these nutrients and lead to deficiency diseases. A study was carried out to access the mutagenic action of sodium azide on the expression of phytic acid and cooking time. These were assessed by the seed analysis of the first mutant generation in pinto, red kidney and navy genotypes of Phaseolus vulgaris. Four doses of the mutagen were applied in concentrations of 0.1M, 0.04M, 0.03M and 0.02M for each genotype and planted in a randomized complete block design. The results showed that phytic acid decreased in the pinto for all the doses except at 0.1M, where it showed an increase (17.56mg/100g) of 60.21%. Phytic acid also showed a decrease (3.9mg/100g, 61.15%) at the highest dose of sodium azide (0.1M) in the red kidney. Phytic acid content in the seeds of navy showed a decrease at all the doses.............
Key words: Sodium azide, phytic acid, cooking time, Phaseolus vulgaris.
[1]. Adesoye, A.I. and O.A. Ojobo, 2012. Genetic Diversity Assessment of Phaseolus vulgarisL. Landraces in Nigeria's Mid-altitude Agroecological Zone. International journal of biodiversity and conservation. 4(13):453-460
[2]. Balamaze, J., Muyonga, J.H., Kyamu Hangire, W.M., Kikafunda, J.K., Nakinbugore, D., and M. Ugen, 2008. Influence of Variety Growth Location, and Storage Conditions and Development of
[3]. Hard To Cook Defect in Common Bean (Phaseolus vulgaris)African Journal of Food Agriculture, Nutrition and Development. 8(3).
[4]. Blair, M.W., Zquierdo, P.I., Astudillo C. and M.A Grusak, 2013. A Legume Biofortification Qrandary: Variability and Genetic Control of Seed Coat Nutrient Accumulation in Common Beans. Plant sci. 4:275
[5]. Gupta, U.S., 2011.Phaseolus Beans:What's New About Crop Plants: Novel Discoveries of the 21st Century.Science Publishers. pp227-300.
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| Paper Type | : | Research Paper |
| Title | : | IL28B Gene polymorphisms in prediction of PEG-IFN-α therapy in HCV Egyptian patients |
| Country | : | Egypt |
| Authors | : | Magdy Mahmoud Mohameda || Osama Ahmed Khalafallahb || Eslam M.A. El-Nahreryc || Nourhan Mohammed Fouadc |
Abstract: Background: Chronic HCV is one of the commonest reasons for ceaseless liver malady worldwide with around 15% of populace tainted in Egypt. Certain SNPs present close to the IL28B gene were found to affect the spontaneous clearance and also treatment result of HCV. To analyze the relationship between different IL28B variations and the relapse of HCV disease after consolidated treatment with ribavirin and pegylated interferon (pegIFN). Methods: Forty HCV genotype all patients got 1.5 mg/kg/week peg-interferon alfa-2b in addition to 800-1400 mg/d ribavirin (weight-balanced) for 48 weeks............
Key words: HCV; IL28B polymorphism; relapsers.
[1]. sheet Wf. World Health Organization. WHO fact sheet 2000;164.
[2]. El-Zanaty F, Way A. Feeding practices and micronutrient supplementation. Egypt Demographic and Health Survey 2008. 2009:166-75.
[3]. Health. EMo. Report Annual. Accessed October. 2013;13.
[4]. Lehman EM, Wilson ML. Epidemiology of hepatitis viruses among hepatocellular carcinoma cases and healthy people in Egypt: A systematic review and meta‐analysis. International journal of Cancer. 2009;124(3):690-7.
[5]. Nakano T, Lau GM, Lau GM, Sugiyama M, Mizokami M. An updated analysis of hepatitis C virus genotypes and subtypes based on the complete coding region. Liver international. 2012;32(2):339-45..
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| Paper Type | : | Research Paper |
| Title | : | Extraction, Characterization and Application of Gelatin fromCarcharhinus amblyrhyncho and Sphyraena barracuda |
| Country | : | India |
| Authors | : | Anchana devi || Kamatchi P || Leela .K |
Abstract: Demand of gelatin for food and pharmaceutical application is increasing because it is one of the most widely used food ingredients. Fish gelatin represents one of the important types of gelatin from other sources other than mammalian gelatins and it may thus be considered as an alternative to mammalian gelatin for use in various food and pharmaceutical applications. The main objective of the study is to extract gelatin from fish skin of Carcharhinus amblyrhyncho (shark) and Sphyraena barracuda (sheela). Gelatin extracted by acid and alkaline treatment and confirmed by gelatinase test and FT-IR analysis............
Key words: Acid and alkaline treatment, Fish skin, Gelatin, Gelatinase test, FT-IR analysis
[1]. See, S. F., Hong, P. K., Ng, K. L., Wan Aida, W. M. and Babji, A. S. 2010, 'Physicochemical properties of gelatins extracted from skins of different freshwater fish species', International Food Research Journal 17: 809-816 .
[2]. K. Shyni, G.S. Hema, G. Ninan, S. Mathew, C.G. Joshy, P.T. Lakshmanan 2014, 'Isolation and characterization of gelatin from the skins of skipjack tuna (Katsuwonus pelamis), dog shark (Scoliodon sorrakowah), and rohu (Labeo rohita) ', Food Hydrocolloids 39.
[3]. Nik Aisyah NM, Nurul H, Azhar ME and Fazilah A. 2014, 'Poultry as an Alternative of GelatinSource'. Health and the Environment Journal, 2014, Vol 5, No 1Pp37-49 37.
[4]. Abdalbasit Adam Mariod, Hadia Fadol Adam. 2013, 'Review: Gelatin, Source, Extraction and Industrial, Applications', Acta Sci. Pol., Technol. Aliment. 12(2), 135-147.
[5]. M. Abd Elgadir, Mohamed E. S. Mirghani and Aishah Adam 2011, 'Fish gelatin and its applications in selected pharmaceutical aspects as alternativesource to pork gelatin', Journal of Food, Agriculture & Environment Vol.11 (1): 73-79. 2013.
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| Paper Type | : | Research Paper |
| Title | : | Review ofBiological Methods for Hazardous Waste Treatment |
| Country | : | India |
| Authors | : | G. Sood || N. Chitre || K.C. Dabhadkar |
Abstract: About 9.3 million metric tons of hazardous waste generated has to be incinerated every yearwhich leave adverse effects on the environment. Biotechnology gives promising ways to not only disinfect and destroy the hazardous, toxic and infectious potential of the waste but also transforms it into material which can be reused. Vermicomposting is used to convert infectious biodegradable waste containing various pathogenic microorganisms to an innocuous waste. Cyanides are peculiar industrial wastes and they can be bio treated by Pseudomonas, achromobacter, flavobacterium, nocardia............
Key words: Biotechnology, Hazardous waste, Waste management
[1]. Ma, Y., Zhao, J., & Yang, B. (2006). Removal of H 2 S in waste gases by an activated carbon bioreactor. International biodeterioration& biodegradation,57(2), 93-98.
[2]. Bohn, H. (1992). Consider biofiltration for decontaminating gases. Chemical Engineering Progress, 88(4), 34-40.
[3]. Delgado, S., Alvarez, M., Rodriguez-Gomez, L. E., &Aguiar, E. (1999). H 2 S generation in a reclaimed urban wastewater pipe. Case study: Tenerife (Spain). Water Research, 33(2), 539-547.
[4]. Chouari, R., Dardouri, W., Sallami, F., Rais, M. B., Le Paslier, D., &Sghir, A. (2015). Microbial analysis and efficiency of biofiltration packing systems for hydrogen sulfide removal from wastewater off Gas. Environmental Engineering Science, 32(2), 121-128.
[5]. Li, J., Ye, G., Sun, D., Sun, G., Zeng, X., Xu, J., & Liang, S. (2012). Performances of two biotrickling filters in treating H2S-containing waste gases and analysis of corresponding bacterial communities by pyrosequencing. Applied microbiology and biotechnology, 95(6), 1633-1641
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| Paper Type | : | Research Paper |
| Title | : | Antimicrobial Activity of Carica papaya, Piper nigrum and Datura stramonium Plants on Drug Resistant Pathogens Isolated from Clinical Specimens |
| Country | : | India |
| Authors | : | Dr. Nagesh Malik || Samreen Ahmed |
Abstract: The aim of the present study was to investigate the antimicrobial activity and phytochemical screening of Carica papaya, Piper nigrum and Datura stramonium plant extracts. Ethanol and Methanol were used as the solvents for extraction. The antimicrobial activities of these plant extracts were tested against the drug resistant Gram-positive and Gram-negative bacterial strains. The bacteria used in the study were Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter baumannii, Salmonella spp., Proteus mirabilis, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecium and Neisseria spp. The ethanol and methanol extracts of leaves of Datura stramonium............
Key words: Antibacterial activity, Drug resistant pathogens, ethanol, methanol, Phytochemical screening
[1]. Ajayi I. A., Ajibade O. and Oderinde R. A. 2011. Preliminary Phytochemical Analysis of some Plant Seeds. 1.
[2]. Behboud Jafari, Amirreza Ebadi, Babak Mohammadi Aghdam and Zarifeh Hassanzade, 2012. Antibacterial Activitiesof Lemon Grass Methanol Extract and Essence on Pathogenic Bacteria. American-Eurasian J. Agric. & Environ. Sci. 12:1042-1046.
[3]. Bipul Biswas, Kimberly Rogers, Fredrick McLaughlin, Dwayne Daniels and Anand Yadav. 2013. Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria. 2013:7.
[4]. C. Baskaran, V. Ratha bai, S.Velu and Kubendiran Kumaran. 2012. The efficacy of Carica papaya leaf extract on some bacterial and a fungal strain by well diffusion method. Asian Pacific Journal of Tropical Disease. 658-662.
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| Paper Type | : | Research Paper |
| Title | : | Review of Production of Biofuels |
| Country | : | India |
| Authors | : | E. P. Godbole || K. C. Dabhadkar |
Abstract: Biofuels being derived from biomass, they are one of the most renewable and biodegradable sources of energy present. It is very important to harvest this technology due to the increasing fear of the impact that the conventional petro fuels have on the environment. This study reviews some of the methods for the production of biofuel. Some of the existing and emerging technologies have been discussed. It can be seen in the course of the paper that the production processes are extremely environment friendly as compared with the production processes of conventional fuels.............
Key words: Biofuels, Biodiesel, Microalgae, Genetic engineering, Bioethanol.
[1]. Lee, Sung Kuk, et al. "Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels." Current opinion in biotechnology 19.6 (2008): 556-563..
[2]. Girard, Philippe, and Abigail Fallot. "Review of existing and emerging technologies for the production of biofuels in developing countries." Energy for Sustainable Development 10.2 (2006): 92-108.
[3]. Cardona, C. A., J. A. Quintero, and I. C. Paz. "Production of bioethanol from sugarcane bagasse: status and perspectives." Bioresource technology101.13 (2010): 4754-4766.
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[5]. Al-Widyan, Mohamad I., and Ali O. Al-Shyoukh. "Experimental evaluation of the transesterification of waste palm oil into biodiesel." Bioresource technology 85.3 (2002): 253-256.