Version-1 (May–June 2017)
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| Paper Type | : | Research Paper |
| Title | : | Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection |
| Country | : | Egypt |
| Authors | : | A.B. Abdel-Razik || I.A. Hammad || E. Tawfik |
 |
: | 10.9790/264X-03030113  |
Abstract: Two thionin genes were isolated from Arabidopsis thaliana (AT1G12660 and AT1G12663). The resulted thionin protein has antifungal activity against potato pathogenic fungi like Alternaria alternata and Rhizoctonia solani. These two thionin genes were respectively inserted in pEGAD vector. After that carried into chitosan nanoparticle in order to be transformed into the two tested potato cultivars (lady and spunta) using intermodal cutting technique of tissue culture. Expressions of thionin transgenes in the transgenic plants were confirmed by RT-PCR. There were two assays were applied to test the role of thionins (in the transgenic potatoes) in resistance to the pathogenic fungi: effect of spore suspension on potato organs..............
Key words: Alternaria alternata, chitosan, Rhizoctonia solani, Solanum tuberosum, Thionin gene, Transformation
[1]. J.E. Edmonds, M.S. Vorontsova, and S. Knapp, Solanaceae, In: Flora of Tropical East Africa, ed. H. Beentje, Royal Botanic Gardens, Kew, 2012.
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[3]. S.I.A Elawady and A.Y. Abdulkheir, An Economic Study of the Growth Determinants for the Egyptian Potatoes Exports to the Global Market, International Journal of Economics and Finance,7(7),2015, 89-97.
[4]. H.A.M.Mahgoub, G.S.A. Eisa and M.A.H. Youssef, Molecular, biochemical and anatomical analysis of some potato (Solanum tuberosum L.) cultivars growing in Egypt, Journal of Genetic Engineering and Biotechnology, Elseveir. 13, 2015,39–49.
[5]. M.A.Draper, G.A. Secor and H.A.Lamey, Managements of potato diseases in the home garden. NDSU; 1994,1-8.
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| Paper Type | : | Research Paper |
| Title | : | Comparison Between Follicular Antimüllerianhormonelevels Between Women's Withpolycystic Ovarian Syndrome And Normal Ovulatory Women Undergoing Intracytoplasmic Sperm Injection (ICSI) Techniques |
| Country | : | Egypt |
| Authors | : | Inam Abdul- Wahid || Affan E Hassan || Basil O Saleh || ZeyadTareq Abdul Razzaq |
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: | 10.9790/264X-03031418 |
Abstract: AMH is a glycoprotein of the transforming growth factor-β (TGF- β) superfamily; it has as an important role in regulation of the ovarian follicle development.AMH Produced by granulosa cells of growing follicles after birth.Polycystic ovary syndrome (PCOS) is the most common cause of menstrual dysfunction, infertility, and hyperandrogenism, and itsprevalence is about 5-10% in female population of reproductive-age women. Follicular fluid (FF) surrounding the oocyte may play an important role in determining oocyte quality and the future potential to achieve fertilization and development of embryo and it provides a very good environment for the development of the oocytes...............
Key words: AHM, PCOS, follicular fluid, ICSI.
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[2]. Anderson AD, Solorzano CM, McCartney CR (2014) Childhood obesity and its impact on the development of adolescent PCOS.SeminReprod Med 32: 202-213.
[3]. Dunaif A, Segal KR, Futterweit W, Dobrjansky A (1989) Profound peripheral insulin resistance, independent of obesity, in polycystic ovary syndrome. Diabetes 38:1165-1174.
[4]. DiamantiKE, DunaifA (2012) Insulin resistance and the polycystic ovary syndrome revisited: an update on mechanisms and implications.Endocr Rev 33: 981-1030.
[5]. Sheehan MT (2004) Polycystic ovarian syndrome: diagnosis and management.Clin Med Res 2: 13-27.
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| Paper Type | : | Research Paper |
| Title | : | Application of Sodium Alginate Hydrogel |
| Country | : | China |
| Authors | : | Wael jumah Aljohani || li wenchao || Muhammad Wajid Ullah || Xianglin Zhang || Guang Yang |
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: | 10.9790/264X-03031931 |
Abstract: Tissue engineering and 3D cell cutler involving the use of three-dimensional scahffold for cell and tissue cultural ,is a promising technique for establishing in vitro culture models that mimc in vivo environment . In vitro models present ethical and cost advantages over in vivo models, and allow for tissue devolpmint.alginate , aliner polysaccharide derived from brown algae .has properties that make it a favorable materials as a 3D extracellular matrix for in vitro cell and tissue models and some of sodium Hydrogyl materials are higly appealing as 3D scaffold for in vitro 3D cell cutlre and tissue culture due their ability to mimic the physical properties native tissue as well as their high porosity for effcient diffusion of proteins and nutrients Alginate hydrogel is widely ysed in tissue engineering application due to its desirable propertis as Biomaterials .
[1]. Enas M. Ahmed, Fatma S. Aggor, Ahmed M. Awad, Ahmed T. El-Are An innovative method for preparation of nanometal hydroxide superabsorbent hydrogel Carbohydr Polym, 91 (2013), pp. 693–69
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[3]. Ratner BD, Bryant SJ. Biomaterials: where we have been and where we are going? Ann Rev Biomed Eng. 2004; 6:41–75. [PubMed: 15255762]
[4]. Williams DF. On the nature of biomaterials. Biomaterials. 2009; 30:5897–5909. [PubMed: 19651435]
[5]. Draget, K.I., et al., Swelling and partial solubilization of alginic acid gel beads in acidic buffer. Carbohydrate Polymers, 1996. 29(3): p. 209-215.5. Langer R, Vacanti JP. Tissue engineering. Science. 1993;260:920–926. [PubMed]Lewis
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| Paper Type | : | Research Paper |
| Title | : | Prevalence and Risk Factors of Diabetes Mellitus in Eket, South-South Nigeria |
| Country | : | Nigeria |
| Authors | : | Iboro Idem || Grace Ukoh || Emmanuel Ekott |
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: | 10.9790/264X-03033235 |
Abstract: Diabetes is a chronic debilitating and costly disease associated with severe threat to the attainment of the internationally agreed developmental goals. Many cases of diabetes die of acute complications, are not diagnosed, or are not presented to hospitals due to poverty and lack of health insurance. This study was a survey carried out in five communities in Eket, South-south Nigeria. A total of 332 individuals, aged ≥ 18 years, out of the 400 individuals randomly selected, presented themselves for the survey. Socio-demographic data, diabetic risk factors including Body Mass Index..............
Keywords: Diabetes mellitus, Body Mass Index, Blood Glucose Concentration, Obesity
[1] Aguocha BU, Ukpabi JO, Onyeonoro U, Njoku P and Ukegbu, AU (2013). Pattern of Diabetes Mortality in a Tertiary Health Facility in South-east Nigeria. African Journal of Diabetes Medicine 21 (1): 14-16
[2] Akinjinmi AA, Adeyooye OA, Akingbade OA, Okerentugba PO (2014). Prevalence of Diabetes Mellitus in Abeokuta, Ogun State, Nigeria. World Journal of Pharmaceutical Research 6(2):73-75
[3] Akinkugbe OO (1997). Non-communicable Disease in Nigeria – Final Report of a National Survey: Federal Ministry of Health National Expert Committee on NCD. Spectrum Books Ltd, Ibadan p. 1-12
[4] Chijioke A, Adamu AN and Makusidi AM (2010). Mortatlity Pattern among Type 2 Diabetes Patients in Illorin, Nigeria. JEMDSA 15: 78-82
[5] Ejike CECC (2014). 'This stroke was sent" – Stroke-related illness concepts and attendant Health-seeking Behaviour of Educated Nigerians. Biochemistry 26 (3):69-75
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| Paper Type | : | Research Paper |
| Title | : | Bio-Preservation of Refrigerated Peeled Shrimp (Parapenaeus Longirostris) Using Cactus Fruit Peels Polyphenolic Extract |
| Country | : | France. |
| Authors | : | Nadia Besbes || Jean-Jacques Joffraud || Ines Ben Khemis || Saloua Sadok |
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: | 10.9790/264X-03033647 |
Abstract: The objective of this study was to evaluate the bio-preservation efficiency of polyphenols extracted from cactus fruit peels in extending the shell life of peeled shrimp during refrigerated (2°C) storage. Changes in microbial, biochemical, and sensorial indicators of cactus polyphenols-treated peeled shrimp (PS) were studied comparatively to a control lot (CS). Results revealed that treatment limited biochemical degradation and reduced the final microbial load in shrimps during storage. They showed the dominance of Brochothrix and Psychrobacter within spoiling bacteria in the CS lot while only limited proliferation of Psychrobacter was observed in PS lot.............
Keywords: Shrimp; Bio-preservation, natural extract, shelf life, bacterial community
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stored in ice. Food Chemistry. 119, 2010, 1527-34. DOI:10.1016/j.foodchem.2009.09.038.
[3] Azeredo, H.M.C. Betalains: properties, sources, applications, and stability – a review. International Journal of Food Science and
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indicators of decomposition in penaeid shrimp. Journal of Food Science, 68, 2003, 2178-85.
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(Dicentrarchus labrax) fillets during storage at 4 °C under vacuum-packed conditions. Journal of Food Safety.
DOI: 10.1111/jfs.12323, 2016.
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| Paper Type | : | Research Paper |
| Title | : | Irisin Gene Polymorphism Rs3480 and Rs1746661and Obesity in Egyptian Populations |
| Country | : | Egypt |
| Authors | : | Azza M. Abdu Allah || Eman Masoud Abd El Gayed || Sahar AF. Hammoudah || Lama Mohamed El-Attar || Walid A. Shehab-Eldin |
|
: | 10.9790/264X-03034854 |
Abstract: The prevalence of obesity is rising worldwide, and presenting a public health problem. Irisin is a novel myokine that is, proteolytically processed from the product of the FNDC5 gene. This study aims at finding out the distribution of FNDC5 rs3480 (A/G) and rs1746661 (G/T) polymorphisms in obese individuals and their association with other clinical and laboratory variables in these persons. This study was done on 141 subjects divided into 2 groups: group I obese group (71) and group II; control group (70). All subjects were submitted to full history taking, general clinical examination and laboratory investigations including BMI, lipid profile, fasting blood glucose, glycated hemoglobin (HbA1c) and genotyping of rs3480 (A/G) and rs1746661 (G/T) gene polymorphisms. There was a significant statistical difference between.............
Keywords: Obesity, Irisin, gene polymorphism, allele, FNDC5
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[2] Hotta K, Nakata Y, Matsuo T, Kamohara S, Kotani K, Komatsu R, et al. . Variations in the FTO gene are associated with severe obesity in the Japanese. J Hum Genet. 2008;53(6):546-553.
[3] Prakash J, Mittal B, Awasthi S, Agarwal CG, SrivastavaN..Hypoadiponectinemia in obesity: association with insulin resistance. Indian J ClinBiochem. 2013. Apr;28(2):158-163.
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| Paper Type | : | Research Paper |
| Title | : | Optimization of Alkaline protease production by fungal isolate using solid state fermentation |
| Country | : | India |
| Authors | : | Vaibhav Shinde || Nagesh Malik |
|
: | 10.9790/264X-03035559 |
Abstract: The purpose of the present study focuses on production of extracellular alkaline proteases by a local fungal isolate through solid state fermentation. The influence of process parameters such as solid substrates, incubation time, carbon and nitrogen source, inducers, activators and inhibitors pH & temperature were studied. Wheat bran was found to be the best among the three solid substrates. The maximum protease production was seen at 120 h of incubation time. The protease production was increased when the medium was supplemented with Lactose as a carbon source. Among the different organic nitrogen sources investigated Yeast extract was found to produce maximum protease. Milk powder was found as the good inducer and EDTA was found as the protease inhibitor. The optimum pH & temperature was 10 and 40o C respectively.
Keywords: Alkaline proteases, Milk powder, pH, Solid state fermentation, Solid substrates
[1] Phadatare S.U., D. V. (1993). High activity alkaline protease from Condiobolous coronatus(NCL86.8.20) Enzyme production and compatibility with commercial detergents. Enz. Microbiol. technol. , 15, 72-76.
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[5] Mitra P., C. R. (1994). Solid state fermentation. J. Sci. Ind. Res. , 55, 134-46,439-42
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| Paper Type | : | Research Paper |
| Title | : | Effect of Cross Linking on the Toxicity of CdTe QDs in Solid/ Liquid Medium |
| Country | : | India |
| Authors | : | Poonam Bichpuria || Anjali Oudhia || Pragya Kulkarni |
|
: | 10.9790/264X-03036064 |
Abstract: Highly luminescent GSH capped CdTe quantum dots (GSH-CdTe QDs) were prepared through a facile microwave (MW) aided wet chemical method. The GSH-CdTe QDs were water soluble and biocompatible. The higher intensity of Photoluminescence (PL) emission can be attributed to the highly crystalline structures of crosslinked GSH-CdTe quantum dots (cGSH-CdTe QDs) observed in the X-ray diffraction (XRD).The SEM micrograph shows densely populated colony of CdTe QDs with almost spherical morphology. The cell death experiment shows that cross linking effectively controls the toxicity of CdTe QDs both in liquid and solid media which makes them suitable for biomedical application. Toxicity control was attributed to the effective surface passivation through cross-linking the GSH shell with 1-ethyl-3-(3-dimethyl aminopropyl carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS).
Keywords: Microwave (MW), Photoluminescence (PL), Toxicity, X-ray diffraction (XRD).
[1]. D.A. Higgins, S. C. Park, Khanh-Hoa Tran-Ba, and T. Ito, Single-Molecule Investigations of Morphology and Mass Transport Dynamics in Nanostructured Materials, Annu. Rev. Anal. Chem. 8, 2015, 193-216.
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| Paper Type | : | Research Paper |
| Title | : | In Vitro Conservation of Tinospora formanii Udayan and Pradeep-A Rare Endemic Plant from Western Ghats |
| Country | : | India |
| Authors | : | Sheema Dharmapal P || T. K. Bindu || P. S. Udayan || K. K. Elyas |
|
: | 10.9790/264X-03036569 |
Abstract: A successful protocol was developed for rapid clonal propagation of Tinospora formanii, a rare endemic medicinal plant of the family menispermaceae through the culture of mature nodal explants. Explants were disinfested with the use of 0.1% mercuric chloride and washed thoroughly with autoclaved distilled water. Solid MS medium was used with addition of different concentrations of 6-benzyl aminopurine (BAP) and indole butyric acid (IBA). The nodal segments inoculated in MS basal medium supplemented with BAP 2.0 mg L-1 and IBA0.5 mg L -1 initiated 2-3 buds in 6-7 days of culture. 95% of explants initiated shoots in this culture. Newly formed shoot clumps cultured on fresh medium with the same concentration of growth regulators as that of the induction medium resulted in shoot proliferation. An average of 12.75 shoots per explant was obtained after 30 days of culture.............
Keywords: Multiple shoots, Nodal explants, Menispermaceae, Tinospora formanii
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| Paper Type | : | Research Paper |
| Title | : | Sensitization to TRAIL and Radioprotective Effect of N-Coumaroyl Tyramine Diacetate from Tinospora Cordifolia Induces Apoptosis and Inhibits Neoangiogenesis to Act as an Anti-Cancer Compound. |
| Country | : | India |
| Authors | : | Yashaswini.B || Bharathi P.Salimath |
|
: | 10.9790/264X-03037077 |
Abstract: Tumor cells show differential sensitivity to cell death induced by TRAIL and also develop resistance to conventional radiation therapy along with several side effects being cytotoxic to normal cells. In this present study, we are focusing on tumor sensitization and radioprotective properties of a novel Phyto-compound isolated, purified from hexane extract and structurally identified as N- Coumaroyl tyramine diacetate (N-CTD), from T.cordifolia. We have isolated a novel small molecule characterized as N- coumaroyl tyramine diacetate from T.cordifolia. Trypan blue exclusion assay was used to study the tumor sensitization effect of N-CTD on TRAIL-resistant cells. The flow cytometric analysis was used to analyze the efficiency of N-CTD to induce apoptosis in TRAIL- resistant cells. To assess the radioprotective effect of N-CTD, the tumor-bearing mice were treated with N-CTD and irradiated with gamma radiation.............
Keywords: Angiogenesis, Tinospora cordifolia (Willd.), TRAIL, Radiation, Ascites tumor, VEGF.
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| Paper Type | : | Research Paper |
| Title | : | An elaborative Analysis of Induced Water stress and role of plant bio-chemicals in regulation and adaptation |
| Country | : | India |
| Authors | : | Dr. Alka Rani |
|
: | 10.9790/264X-03037881 |
Abstract: Environmental stresses bring about a wide range of responses in plants from genetic changes to the changes in growth rate and yield. Tolerance to abiotic stresses is very complex, due to the intricacy of interactions between stress factors and various molecular, biochemical and physiological phenomena affecting plant growth and development. Stress is an altered physiological condition caused by factors that tend to disrupt the equilibrium. Strain is any physical and chemical change produced by the stress. The term "stress" is used with various meanings; the physiological definition is responses in different situations. The flexibility of normal metabolism allows the response initiation to the environmental changes, which fluctuate regularly and are predictable over daily and seasonal cycles. Every deviation of a factor from its optimum does not necessarily result in stress. Stress is a constraint or highly unpredictable fluctuations imposed on regular metabolic patterns to cause injury, disease or aberrant physiology....
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