Series-1 (May – June 2026) May – June 2026 Issue Statistics
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| Paper Type | : | Research Paper |
| Title | : | The CRISPR-Cas9 Paradigm: A Multidimensional Analysis Of Mechanistic Precision, Therapeutic Potential, And Socio-Ethical Governance In Modern Biotechnology |
| Country | : | |
| Authors | : | xxx |
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: | 10.9790/ 264X-1203010108  |
Abstract: The discovery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) platform is a paradigm shift in the background of genomic architecture and molecular therapeutics. Though Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs) provided initial capabilities in the area of gene-editing, they were commonly constrained by their high prices, intricate design, and the lack of throughput. The paper includes an informative analytical inquiry into the CRISPR-Cas9 system, analyzing its mechanistic peculiarities, its disruptive possibilities in biomedical and agricultural.....
[1].
Adli, M. (2018). The CRISPR Tool Kit For Genome Editing And Beyond. Nature Communications, 9(1), 1-13.
[2].
Alberts, B., Johnson, A., Lewis, J., Morgan, D., Raff, M., Roberts, K., & Walter, P. (2022). Molecular Biology Of The Cell (7th Ed.). W. W. Norton & Company.
[3].
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., ... & Liu, D. R. (2019). Search-And-Replace Genome Editing Without Double-Strand Breaks Or Donor DNA. Nature, 576(7785), 149-157.
[4].
Baltimore, D., Berg, P., Botchan, M., Carroll, D., Charo, R. A., Church, G., ... & Witkowski, J. A. (2015). A Prudent Path Forward For Genomic Engineering And Germline Gene Modification. Science, 348(6230), 36-38.
[5].
Charpentier, E., & Doudna, J. A. (2013). Rewriting A Genome. Nature, 495(7439), 50-51
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| Paper Type | : | Research Paper |
| Title | : | Impact Of Drying And Lacto-Fermentation On The Quality Of Tomato Fruits |
| Country | : | Congo |
| Authors | : | Okondza Nohya Delvina || Nkeletela Laurette || Louyindoula Auguste || Bokouango Nyssie || Mabossy Germain || Mananga Vital |
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: | 10.9790/ 264X-1203010915 |
Abstract: Background : The overall objective of this study was to evaluate the effect of several preservation methods on the physicochemical quality of tomato fruits. The experiment focused on two preservation techniques: drying (at 50, 55, and 60°C) and lacto-fermentation. The physicochemical parameters studied were pH, titratable acidity, Brix degree, water content, protein content, and ash content. The results showed that the ideal temperature for drying tomato fruits is 50°C. Indeed, the physicochemical parameters studied showed that at 50°C, the physicochemical...
Key Word: Tomato, preservation, drying kinetics, lacto-fermentation
[1].
EKISSI AC, KOUAME KB, BEUGRE GA, KATI-COULIBALY S. Improvement Of The Shelf Life Of F1 (Boomerang) Tomato Fruits Using Traditional Preservation Techniques. Afrique Science 2021 19(4) 159 – 173. French.
[2].
Ali MY, Sina AAI, Khandker SS, Neesa L, Tanvir EM, Kabir A, Khalil MI, Gan, SH Nutritional Composition And Bioactive Compounds In Tomatoes And Their Impact On Human Health And Disease: A Review. Foods, 2021;10.45.
Https://Dx.Doi.Org/10.3390/Foods10010045
[3].
Seybold C, Frohlich K, Britsch R, Otto K, Bohm VJ Chnages In Contents Of Carotenoids And Vitamin E During Tomato Processing. Journal Of Agricultural And Food Chemistry, 2004 52(23), 7005-7011.
[4].
Bezabih E, Afari V, Nenguwo N, Ayana A, Kebede D, Mohammed H. Characterization Of Pre And Postharvest Losses Of Tomato Supply Chain In Ethiopia. Agriculture And Food Security, 2017; 6:3, 11p
[5].
Atmani, K., Bakhouche, H. 2018. Comparative Study Of Microwave And Oven Drying Of Tomatoes (Lycopersicon Esculentum Mill). Master's Thesis. A. Mira University – Bejaia, Algeria. P. 88.
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| Paper Type | : | Research Paper |
| Title | : | AI Based Multi-Omics Analysis And Structure-Based Drug Discovery Pipeline Targeting Kras4a Mutations For Precision Oncology |
| Country | : | India |
| Authors | : | Sonali || Uma Kumari |
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: | 10.9790/ 264X-1203011624 |
Abstract: Overall analysis presents an integrated computational framework for precision oncology targeting KRAS4A mutations in Non-Small Cell Lung Cancer (NSCLC). The workflow combines Next-Generation Sequencing (NGS), Computer-Aided Drug Design (CADD), Artificial Intelligence (AI), and bioinformatics tools to systematically identify and evaluate potential therapeutic targets. Structural analysis of KRAS4A (PDB ID: 9GGY) revealed a stable conformation with a well-defined binding pocket between switch I and switch II regions. Sequence similarity and evolutionary relationships were assessed using sequence similarity and protein embedding....
Key Word: KRAS4A; NSCLC; NGS; CADD; Artificial Intelligence; ML; Molecular Docking; Precision Oncology; Biopython
[1]. Corral De La Fuente E, Olmedo Garcia ME, Gomez Rueda A, Lage Y, Garrido P. Targeting KRAS In Non-Small Cell Lung Cancer. Front Oncol. 2022 Jan 10;11:792635. Doi: 10.3389/Fonc.2021.792635. PMID: 35083149; PMCID: PMC8784727.Mechanisms And Current Advances In Treating KRAS-Mutated Lung Cancer
[2]. Zhao, R., Shu, Y., Xu, W. Et Al. The Efficacy Of Immunotherapy In Non-Small Cell Lung Cancer With KRAS Mutation: A Systematic Review And Meta-Analysis. Cancer Cell Int 24, 361 (2024). Https://Doi.Org/10.1186/S12935-024-03498-9
[3].
Ritu, K., Kumar, P., Singh, A. Et Al. Untangling The KRAS Mutated Lung Cancer Subsets And Its Therapeutic Implications. Mol Biomed 2, 40 (2021). Https://Doi.Org/10.1186/S43556-021-00061-0
[4]. Ghosh S, Bhuniya T, Dey A, Koley M, Roy P, Bera A, Gol D, Chowdhury A, Chowdhury R, Sen S. An Updated Review On KRAS Mutation In Lung Cancer (NSCLC) And Its Effects On Human Health. Appl Biochem Biotechnol. 2024 Jul;196(7):4661-4678. Doi: 10.1007/S12010-023-04748-8. Epub 2023 Oct 28. PMID: 37897621.
[5].
He, Q. Et Al. (2025) ‘First-Line Treatments For KRAS-Mutant Non-Small Cell Lung Cancer: Current State And Future Perspectives’, Cancer Biology & Therapy, 26(1). Doi: 10.1080/15384047.2024.2441499..
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| Paper Type | : | Research Paper |
| Title | : | NGS And Structure Based Drug Designing Of Natural Compound Against Drug Resistant EGFR In Non-Small Cell Lung Cancer |
| Country | : | India |
| Authors | : | Riyaz Gurung || Uma Kumari |
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: | 10.9790/ 264X-1203012534 |
Abstract: Mutations in Epidermal Growth Factor Receptor drives disease progression and treatment resistance in Non-small Cell Lung Cancer. EGFR is a well-documented therapeutic target for management of NSCLC. Our research combines Next Generation Sequencing (NGS) and Structure Based Drug Design (SBDD) approaches to identify natural compounds as potential therapeutical. In our study, we targeted the triple mutated EGFR kinase domain (L858R T790M/C797S) using its crystal structure (PDB ID: 9D3W) to design EGFR tyrosine kinase inhibitors. NGS examined...
Key Word: Next-Generation Sequencing (NGS), Structure Based Drug Design (SBDD), Natural products, EGFR Mutants, Non-small Cell Lung Cancer (NSCLC), Molecular Docking, Targeted Therapy, Precision Medicine
[1].
Bajaj, T., & Kumari, U. (2025). Issue 3 Www.Jetir.Org (ISSN-2349-5162). In JETIR2503614 Journal Of Emerging Technologies And Innovative Research (Vol. 12). Www.Jetir.Org
[2].
Bandbe, T., Saikia, J., & Kumari, U. (N.D.). NGS Analysis Human Papillomavirus Type 18 E2 DNA-Binding Domain Bound To Its DNA Target With Biopython.
[3].
Bindod, H. V., Hatwar, P. R., Bakal, R. L., & Dafe, V. N. (2025). A Comprehensive Review Of Cancer: Types, Pathophysiology, Diagnosis And Treatments. Journal Of Drug Delivery And Therapeutics, 15(5), 114–122. Https://Doi.Org/10.22270/Jddt.V15i5.7104
[4].
Ferro, A., Marinato, G. M., Mulargiu, C., Marino, M., Pasello, G., Guarneri, V., & Bonanno, L. (2024). The Study Of Primary And Acquired Resistance To First-Line Osimertinib To Improve The Outcome Of EGFR-Mutated Advanced Non-Small Cell Lung Cancer Patients: The Challenge Is Open For New Therapeutic Strategies. In Critical Reviews In Oncology/Haematology (Vol. 196). Elsevier Ireland Ltd. Https://Doi.Org/10.1016/J.Critrevonc.2024.104295.