Series-1 (Jan. – Feb. 2023)Jan. – Feb. 2023 Issue Statistics
- Citation
- Abstract
- Reference
- Full PDF
- Index Page
- Cover Paper
Abstract: Background: Probiotics are live microbial food constituents that affect the microflora and provide health benefits to the host when consumed in sufficient quantities. Laphet (Myanmar's traditional fermented tea leaves) is one of the most consumed daily snack throughtout Myanmar and contains Latic Acid Bacteria (LAB) which play an important role in probiotics. The aim of this study was to use the catalase assay and the type of fermentation to assess the features and biochemical properties of LAB. Materials and Methods: Lactic acid bacteria (LAB) were identified using particular media, especially MRS Agar, and the antimicrobial activity was tested using test bacteria Escherichia coli O157, Staphylococcus aureus. Results: The study's findings were as follows: The result of the catalase......
Keywords: isolation, characterization, probiotics, bacteria, Laphet
[1]. T. Han and K. N. Aye, "The legend of laphet: A Myanmar fermented tea leaf," J. Ethn. Foods, vol. 2, no. 4, pp. 173–178, 2015, doi: 10.1016/j.jef.2015.11.003.
[2]. B. Bo, S. A. Kim, and N. S. Han, "Bacterial and fungal diversity in Laphet, traditional fermented tea leaves in Myanmar, analyzed by culturing, DNA amplicon-based sequencing, and PCR-DGGE methods," Int. J. Food Microbiol., vol. 320, no. July 2019, 2020, doi: 10.1016/j.ijfoodmicro.2020.108508.
[3]. R. Amelia, K. Philip, Y. E. Pratama, and E. Purwati, "Characterization and probiotic potential of lactic acid bacteria isolated from dadiah sampled in West Sumatra," Food Sci. Technol., vol. 41, pp. 746–752, 2021, doi: 10.1590/fst.30020.
[4]. M. Ayyash, A. S. Al-Dhaheri, S. Al Mahadin, J. Kizhakkayil, and A. Abushelaibi, "In vitro investigation of anticancer, antihypertensive, antidiabetic, and antioxidant activities of camel milk fermented with camel milk probiotic: A comparative study with fermented bovine milk," J. Dairy Sci., vol. 101, no. 2, pp. 900–911, 2018, doi: 10.3168/jds.2017-13400.
[5]. O. Yerlikaya, "Probiotic potential and biochemical and technological properties of Lactococcus lactis ssp. lactis strains isolated from raw milk and kefir grains," J. Dairy Sci., vol. 102, no. 1, pp. 124–134, 2019, doi: 10.3168/jds.2018-14983.
- Citation
- Abstract
- Reference
- Full PDF
Abstract: As food and medicinal ingredient, Bitter gourd (Momordica charantia) (belonged to the Cucurbitaceae family), has long been used in several Asian countries. To assess and evaluate the phytochemicals analysis and pharmacological activities of methanolic extract of dried leaves of Momordica charantia, the present study has been conducted, in where we have been used Folin-Ciocalteu's colorimetric assay, aluminium chloride colorimetric assay, Ferrous-ferrozine complex inhibition assay, and agar disc diffusion method. The maximal phenolic contents got was 20.22±0.27 mg of GAE/g of dry extract and the total flavonoid contents got was 38.78±0.74 mg of CE/g of dry......
Keywords: Momordica charantia, phytochemicals analysis, pharmacological activity, Folin-Ciocalteu's colorimetric assay, agar disc diffusion method
[1]. Venkatesh Kumar, R. and S. Chauhan, Mulberry: life enhancer. Journal of Medicinal Plants Research, 2008. 2(10): p. 271-278.
[2]. Kubola, J. and S. Siriamornpun, Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food chemistry, 2008. 110(4): p. 881-890.
[3]. Wang, S., et al., How natural dietary antioxidants in fruits, vegetables and legumes promote vascular health. Food Research International, 2011. 44(1): p. 14-22.
[4]. Panche, A.N., A.D. Diwan, and S.R. Chandra, Flavonoids: an overview. Journal of nutritional science, 2016. 5: p. e47.
[5]. Huang, W.-Y., Y.-Z. Cai, and Y. Zhang, Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutrition and cancer, 2009. 62(1): p. 1-20..
- Citation
- Abstract
- Reference
- Full PDF
Abstract: Lignocellulose is a renewable resource or organic matter. It consists of three types of polymers viz., cellulose, hemicelluloses and lignin that are strongly intermeshed and chemically bonded by non-covalent forces and by covalent cross-linkage. Many fungi are capable of degrading cellulose and hemicelluloses and utilize as chief carbon and energy sources. However, some filamentous fungi have evolved with their ability to degrade lignin. These are known as white-rot fungi, which possess the unique ability to degrade lignin into CO2. The Lignocellulosic material is composed of cellulose, hemicelluloses, pectin and lignin. Cellulose is a polysaccharide composed of linear glucan chains that are linked together by β-1, 4-glycosidic linkages with cellobiose residues as the repeating units......
Keywords: Lignocellulose, Agaricus bisporus, Hemicellulose, Cellulose, Lignin, Biodegradation, Mineralization.
[1]. Kim S, Dale BE (2004): Global potential bioethanol production from wasted crops and crop residues. Biomass and Bioenergy, 26:361-375.
[2]. Mehdi Dashtban, Heidi Schraft, Wensheng Qin (2009): Fungal Bioconversion of Lignocellulosic Residues; Opportunities & Perspectives Int. J. Biol. Sci. 5(6):578-595
[3]. Pérez J, Muñoz-Dorado J, De-la-Rubia T, Martínez J. (2002): Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview. Int Microbiol, 5:53–63.
[4]. Bennett JW, Wunch KG, Faison BD (2002): Use of fungi in biodegradation. In: Hurst CJ, editor. Manual of Environmental Microbiology.Washington DC: AMS press; p. 960–71.
[5]. Martinez G, Larrondo N, Putman N, Gelpke MDS, Huang K, Chapman J, et al., (2004): Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nature Biotechnol; 22:1–6..
- Citation
- Abstract
- Reference
- Full PDF
Abstract: Sugarcane Bagasse is fibrous structure obtained by crushing the sugarcane stem and extracting juice. It is used in the production of enzymes, amino acids, drugs, ethanol and single cell protein. Sugarcane Bagasse is considered as a pure waste material which can be used to extract cellulose and nanocellulose particles. Cellulose is a linear polysaccharide consisting of β-D-glucose chains joined together by β-1, 4-glycosidic linkages with cellobiose residues as the repeating unit at different degree of polymerization. In the present investigation......
Keywords: Sugarcane Bagasse, Cellulase, Biodeterioration, Soil Fungi
[1]. Michel, D, B. Bachelier, J.-Y. Drean, O. Harzallah (2013): Preparation of cellulosic fibers from sugarcane for textile use, in: Conference Papers in Materials Science, Guimare aes.
[2]. Pandey, A, C.R. Soccol, P. Nigam, V.T. Soccol (2000): Biotechnological potential of agroindustrial residues. I: sugarcane bagasse, Biores. Technol. 74: 69–80.
[3]. Hern_andez-Salas, J, M.S. Villa-Ramı_rez, J.S. Veloz-Rend_on, K.N. Rivera-Hern_andez, R.A. Gonz_alez-C_esar, M.A. Plascencia-Espinosa, S.R. Trejo-Estrada (2009) : Comparative hydrolysis and fermentation of sugarcane and agave bagasse, Biores. Technol. 100: 1238–1245.
[4]. Loh, Y. R, D. Sujan, M.E. Rahman, C.A. Das (2013): Sugarcane bagasse-the future composite material: a literature review, Res. Cons. Recycl. 75: 14–22.
[5]. Eriksson, K. E (1990): Biotechnology in the pulp and paper industry. Wood Sci. Technol, 24: 79-101
- Citation
- Abstract
- Reference
- Full PDF
Abstract: Background:In this work, a practical activity was developed, including the construction of graphs, evaluating the power or the buffering capacity of a buffer solution. This concept, although it is presented in several Undergraduate Courses, is considered very abstract and difficult to assimilate by most students. Thus, it is expected that the activity will be a motivating factor in the teaching-learning process of chemical equilibrium, and in turn buffer solution. It was also possible to review the importance of a buffer solution, using blood tissue as the main example.Materials and Methods: To visualize the buffering power of the buffer solution, successive aliquots of acidic or alkaline solutions......
Keywords: teaching-learning process, biochemistry, potentiometry
[1]. Harris, D. C; Lucy, C. A. Quantitative Chemical Analysis. 10th Edition. New York: Macmillan Publishers, 2020, 825p.
[2]. Marconato, J. C.; Franchetti, S. M. M.; Pedro, R. J., Buffer Solution: An Experimental Proposal Using Low. Química Nova na Escola, v. 20, 2004, p. 59-62.
[3]. Brown, T. L., Le May JR, H. E., Bursten, B. E., Murphy, C. J.; Woodward, P. M.; Stoltzfus, M. W. Chemistry: The Central Science. 14th Edition.London: Pearson, 2017,1248p.
[4]. Framer, M. Transição da Respiração Aquática para a Aérea: Efeitos do CO2 sobre a função da Hemoglobina. Supl. Acta Amazônica, v. 8, n. 4, 1978, p.143-151
[5]. Fiorucci, A. R.; Hebert, M.; Soares, F. B.; Cavalheiro, E. T. G. The Concept of Buffer Solution. Química Nova na Escola, v.13, 2001, p.18-21.
[6]. Voet, D; Voet, J. G.; Pratt, C. W.Fundamentals of Biochemistry: Life at the Molecular Level, 5th Edition. New York: Wiley, 2016, 1186p.
- Citation
- Abstract
- Reference
- Full PDF
Abstract: Nanoparticles have a bigger surface area to volume ratio than larger particles do because their size is so much smaller. This is because their size is so much smaller. When it comes to the catalytic activity of AgNPs, as well as other factors associated to catalysis, such as their antibacterial activity, the specific surface area of the AgNPs is of the utmost importance. Traditional forms of medicine have made use of several plants for treatment for a very long time. The preservation of traditional cultures, the care of the community's health, the discovery of new medicines, and the protection of biodiversity can all benefit from the collection of ethno-botanical data on medicinal plants.....
Keywords: Growth , Hormones , Callus, Induction, Nanoparticles
[1]. Aslam et al., 2010, Gupta et al., 2018, Kabesh et al., 2015 In vitro antiplasmodial activity of extracts and fractions from seven medicinal plants used in the Democratic Republic of Congo
[2]. Kabesh et al., 2015 Antimalarial activity of 20 crude extracts from nine African medicinal plants used in Kinshasa, Congo J Ethnopharmacol(1999)
[3]. Aslam et al., 2010, Gupta et al., 2018, Kabesh et al., 2015 Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens Colloids Surf B Biointerfaces (2011)
[4]. Lounasmaa& Galambos1989 "Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications – an updated report," Saudi Pharmaceutical Journal, vol. 24, pp. 473–484, 2016. View at: Publisher Site | Google Scholar
[5]. Scarano et al., 2002 "Phytochemical analysis and Antioxidant activity of Catharanthus roseus Flower extract in different solvent," International Journal of Advanced Scientific Research and Management, vol. 1, p. 11, 2018. View at: Google Scholar.
- Citation
- Abstract
- Reference
- Full PDF
Abstract: For biochemical research, various Withania coagulans and Withania somnifera plant sections from the Solanaceae family were used. Although they are mainly regarded as dangerous plants and only occasionally utilized in Pakistani folk medicine, these are wild plants. The yield of menthol extracted from the roots, stems, and leaves of both plants was also calculated. W. coagulans leaves had a higher extract yield percentage (7.6%) than the stem (6.3%) or root (6.3%). All portions of W. coagulans had a higher percent extract yield than the same parts.....
Keywords: Medicinal plants, secondary metabolites, therapeutic agents, ashwagandha
[1]. Abdelhamid, M. S., Kondratenko, E. I., & Lomteva, N. A. (2015). GC-MS analysis of phytocomponents in the ethanolic extract of Nelumbo nucifera seeds from Russia. Journal of applied pharmaceutical science, 5(4), 115- 118.
[2]. Abdul, Q. A., Choi, R. J., Jung, H. A., & Choi, J. S. (2016). Health benefit of fucosterol from marine algae: a review. Journal of the Science of Food and Agriculture, 96(6), 1856-1866.
[3]. Abubakar, M. N., & Majinda, R. R. (2016). GC-MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines, 3(1), 3.
[4]. Abu-Dalo, M. A., Al-Rosan, S. A., & Albiss, B. A. (2021). Photocatalytic degradation of methylene blue using polymeric membranes based on cellulose acetate impregnated with ZnO nanostructures. Polymers, 13(19), 3451.
[5]. Achi, N. K., & Ohaeri, O. C. (2015). GC-MS determination of bioactive constituents of the methanolic fractions of Cnidoscolus aconitifolius. British Journal of Pharmaceutical Research, 5(3), 163.
- Citation
- Abstract
- Reference
- Full PDF
Abstract: The goal of this study was to evaluate the effectiveness of Trigonella foenum-graecum extracts against Methicillin-Resistant Staphylococcus aureus (MRSA) when coupled with antibiotics. Alkaloids, phenols, tannins, flavonoids, and saponins were all found, according to the phytochemical qualitative analysis. The colony suspension method was used to standardize the MRSA strain for the study of the antibiotic susceptibility. The suspensions had a concentration of 1.5 106 CFU/mL because they matched the 0.5 McFarland criteria. Under the guidelines of the Kirby-Bauer test, antimicrobial susceptibility tests were performed on Mueller Hinton agar using both the
Keywords: Methicillin-Resistant Staphylococcus aureus (MRSA); phytochemicals; antibiotics; Trigonella foenum-graecum
[1]. Gajdács M. The continuing threat of Methicillin-Resistant Staphylococcus aureus. J Antibiot 2019;8:52.
[2]. Boucher H, Miller LG, Razonable RR. Serious infections caused by Methicillin- Resistant Staphylococcus aureus. Clin. Infect. Dis. 2010;2:183-197.
[3]. Liu T, Zhang Y, Wan Q. Methicillin- Resistant Staphylococcus aureus bacteremia among liver transplant recipients: epidemiology and associatedrisk factors for morbidity and mortality. Infect. Drug. Resist 2018;11:647.
[4]. Bukowski M, Wladyka B, Dubin G. Exfoliative toxins of Staphylococcus aureus. Toxins 2010;2:1148-1165.
[5]. Kali A, Stephen S, Umadevi S, Kumar S, Joseph NM, Srirangaraj S. Changing trends in resistance pattern of methicillin resistant Staphylococcus aureus. J. Clin. Diagn. Res 2013;7:1979..
- Citation
- Abstract
- Reference
- Full PDF
Abstract: Antibiotics and synthetic medications that have major side effects are not without their drawbacks, but medicinal plants are a rich source of potentially beneficial phytochemical substances. The waste materials from the fruits, Punica granatum peel and Vitis vinifera seeds, were extracted in the current study using ethanol as the solvent. The antibacterial potential and phytochemical content of the ethanolic extract of both fruit components were examined. A qualitative phytochemical examination of the chosen extracts revealed the presence of powerful bioactive components as polyphenols, terpenoids, flavonoids, tannins, alkaloids, cardiac glycosides, carbohydrates....
Keywords: Punica granatum; Vitis vinifera; Antibacterial activity; phytochemical analysis; Fruit waste; disc diffusion method.
[1]. Adhami, V.M., and Mukhtar, H. 2006. Polyphenols from green tea and pomegranate for prevention of prostate cancer. Free. Rad. Res. 40(10): 1095- 104.
[2]. Ahmad, I., Z. Mehmood and Mohammad,F. 1998. Screening of some Indian medicinal plants for their antimicrobial properties. J. Ethnopharmacol. 62: 183 93.
[3]. Ashok Kumar, K., and Vilayalakshmi K. 2011. GC-MS Analysis of phytochemical constituent in Ethnolic extract of Punica granatum peel and Vitis vinifera seeds. Int.J.Pharma. Bio sci. 2 (4): 461 468.
[4]. Bandow, J. E., H. BrotzLeichert, L.I.O. Leichert, H. Labischinski and Hecker, M. 2003. Agent. Chemother. 47: 948-955.
[5]. Bauer, A.W., M.M. Kriby, J.C. Sherris and Truck, M .1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin.Pathol. 45: 493-6..
- Citation
- Abstract
- Reference
- Full PDF
Paper Type | : | Research Paper |
Title | : | Synthesis of HSP for Thermo tolerance in Plant s |
Country | : | India |
Authors | : | Dr. Reena Tomar |
: | 10.9790/264X-09016267 |
Abstract: Organisms produce a modest number of highly conserved heat shock proteins in response to environmental stress. The development of thermotolerance is closely tied to the synthesis of these proteins in a wide range of organisms, including bacteria, plants, invertebrates, and vertebrates. The conclusion that heat shock proteins are necessary for stress tolerance and that the principal heat shock protein in plants has no impact on normal growth or physiology is thus directly supported by these data. The results also suggest that an organism's natural environment may have an impact on whether or not it exhibits a heat shock response.
Keywords: Heat shock protein, Thermotolerance, Stress tolerance, Physiology etc.
[1]. Abdul-Baki, A A. (1991, November 1). Tolerance of Tomato Cultivars and Selected Germplasm to Heat Stress. https://scite.ai/reports/10.21273/jashs.116.6.1113
[2]. Burke, J.J., Hatfield, J.L., Klein, R.P., Mullet, J.E., 1985. Accumulation of heat shock proteins in field-grown cotton. Plant Physiol. 78, 394–398.
[3]. Charng, Y.Y., Liu, H.C., Liu, N.Y., Chi, W.T., Wang, C.N., Chang, S.H., Wang, T.T., 2007. A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant Physiol. 143, 251–262.
[4]. Chen, W.Q.J., Zhu, T., 2004. Networks of transcription factors with roles in environmental stress response. Trends Plant Sci. 9, 591– 596.
[5]. Clos, J., Westwood, J.T., Becker, P.B., Wilson, S., Lambert, K., Wu, C., 1990. Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation. Cell 63, 1085–1097.