Version-2 (May–June 2017)
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| Paper Type | : | Research Paper |
| Title | : | 5-Azacytidine Promotes Differentiation of Bone Marrow Derived Mesenchymal Stem Cells into Troponin Positive Cardiomyocyte‑Like Cells in Vitro |
| Country | : | Egypt |
| Authors | : | Iman A.Ahmedy MD || Waleed M. Fathy MD || Rasha I.Noreldin MD || Wassam EL Din Elshafey MD |
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: | 10.9790/264X-03030111  |
Abstract: Objective: The aim of this work is to differentiate Mesenchymal stem cells derived (MSCs) from adult human bone marrow into cardiomyocyte in vitro using 5- azacytidine. Background: MSCs provide a promising approach for cellular therapy because of their self-renewing properties and multipotent differentiation capacity. Owing to the limited ability of the injured heart to regenerate cardiomyocytes. Cardiomyocyte transplantation has been advocated as a possible treatment for heart failure. Their other benefits on a myocardiac infarct is owed to neo-vascularization, because they can differentiate also into endothelial cells. Methods: MSCs were cultured from bone marrow aspirate and detected morphologically and by flow-cytometric analysis of surface markers CD44 and CD 34 then differentiated to cardiomyocytes by using 5-azacytidine .The cardiomyocytes were detected morphologically and by flowcytometric analysis of Vimentin and Troponin and immunofluorescence analysis for Cardiac alpha tropomyosin..............
Key words: 5-azacytidine,cardiac alpha tropomyosin, cardiogenesis,mesenchymal stem cells, troponin
[1] Friedenstein AJ, Chailakhyan RK, Latsinik NV, Panasyuk AF,Keiliss-Borok IV.Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo. Transplantation1974; 17:331-340.
[2] Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284:143-147.
[3] Souza Neto JL, Araujo FI, Rego AC, Dominici VA, Azevedo IM, Egito ES, Brandao-Neto J, Medeiros AC. Effects of simvastatin in abdominal sepsis in rats. Acta Cir Bras 2006; 21:8-12.
[4] Minguell J., Erices A., and Conget P., "Mesenchymal stem cells," Experimental Biology and Medicine 2001; 226:507–520.
[5] Park K. S., Lee Y. S., and Kang K. S., "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood," Journal of Veterinary Science 2006 ;7: 343–348.
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| Paper Type | : | Research Paper |
| Title | : | An investigative study in Dima Hasao District, Assam: Study of association of alcoholic drink and ALD using three biomarkers of liver-AST, ALT and Bilirubin. |
| Country | : | India |
| Authors | : | Mandira Lohar || Pimily Langthasa |
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: | 10.9790/264X-03031214 |
Abstract: Liver is one of the vital organ of the body and any kind of chemical exposure to it may lead to its toxicity. The use of alcohol is being a common practice around the world alternating the life style of the people from normal to dependency towards it. As a result, people are emerging with alcoholic liver health issues. Many liver biomarkers like AST, ALT, GGT, Bilirubin etc. are being used to assess the liver health status but biopsy is the most accurate one. Till date ALT, AST and Bilirubin is considered as the primary LFT test to evaluate any kind of liver injury. The people of Dima Hasao District, Assam, India practices a typical traditional alcoholic drink called Judima and Juharu which they consider it to be harmless to liver health. Therefore, the main objective of this study is to assess the liver health status of the people of Dima Hasao District, using three circulating biomarkers of liver- AST, ALT and Bilirubin............
Key words: Alcoholic Liver Disease, Hepatic Damage, ALT, AST and Bilirubin
[1]. O'Shea RS, Dasarathy S, McCullough AJ, 2010. "Alcoholic liver disease" (PDF). Hepatology 51 (1): 307–28.
[2]. Hyder M A, Hasan M, Mohieldein A, Eur. Jr. of Expt. Bio. 2013, 3(2): 280-284
[3]. Desmond Burke M, Clin Lab Med , 2002:22: 377–39
[4]. Bird GL. Investigation of alcoholic liver disease: Baillier Clin Gastroenterol 1993;7:663–82
[5]. Savolainen, E.-R., Goldberg, B., Leo, M. A., Velez, M. and Lieber, C. S. (1984).Diagnostic value of serum procollagen peptide measurements in alcoholic liver disease. Alcoholism: Clinical and Experimental Research 8,384–389.
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| Paper Type | : | Research Paper |
| Title | : | Comparative Study on Nutritive Content of Finger Millet-Wheat Composite Bread Fermented With Lactic Acid Bacilli and Yeast |
| Country | : | India |
| Authors | : | Ramesh Mythrayee || Amritkumar Pavithra |
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: | 10.9790/264X-03031521 |
Abstract: Different forms of bread enriched with millets (composite breads) are being tried, to improve the nutritive quality of the bread. In the present study, one of the traditional millets of south India, Finger millet (Ragi), was mixed with whole wheat flour and fermented with lactic acid bacteria (LAB) as well as Baker's yeast. The composite breads were evaluated for physical, nutritional and shelf life properties, and compared with the commercially available white bread. The nutritive content of both the types of composite bread were superior to the commercially available white bread, serving as a rich source of calcium (296%mg as against 40%mg in white bread). They also showed better shelf-life due to their lower gluten and moisture content. Finger millet-whole wheat composite bread fermented with Baker's yeast had higher Vitamin B group (1.09% mg) compared to the same fermented using LAB (0.40% mg) and the white bread (0.15% mg). This study attempted to formulate alternative and healthy bread making without use of chemicals associated with modern bread baking.
Key words: Finger millet, lactic acid bacteria, Vitamin B group, composite bread, whole wheat flour
[1] Matz, S. A. The chemistry and technology of cereals as food and feed (AVI Books pp. 1–2; 1995).
[2] Giannou, V., Kessoglou, V., Tzia, C. Quality and safety characteristics of bread made from frozen dough. Trends in Food Science and Technology,14 (3), 2003, 99–108.
[3] Rollán G, Gerez CL, Dallagnol AM, Torino MI, Font G Update in bread fermentation by lactic acid bacteria. Méndez-Vilas A (ed) Current Research, Technology and Education Topics in Applied Microbiology and Biotechnology, 1st edn. Fomatex, Spain, 2010, 1168-1174.
[4] Salovaara H. Lactic acid bacteria in cereal-based products. Salminen S, von Wright A, ed. Lactic acid bacteria— Microbiology and functional aspects. (New York, NY: Marcel Dekker, 115-137, 1988).
[5] Hammes WP, Gänzle MG. Sourdough breads and related products. Woods BJB, ed. Microbiology of Fermented Foods Vol. 1. (London: Blackie Academic/Professional, 199-216, 1998)
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| Paper Type | : | Research Paper |
| Title | : | Assessment of serum biomarkers of oxidative stress in patients with chronic renal failure |
| Country | : | India |
| Authors | : | Dr. Sarala Devi Tenepalli || Dr. Karunasree Yerrajwala |
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: | 10.9790/264X-03032224 |
Abstract: Chronic renal failure (CRF) is becoming a major health challenges among worldwide. Incidence of the disease is alarming, because an insufficient therapeutic methods. Based on the estimated glomerular filtration rate (eGFR), the CRF is being classifying in to numerous phases. Recent guidelines classified the severity of CRF into 5 phases. First stage is being the mildest and fifth stage being a severe illness with less life expansion. The present study was designed to assess the link between CRF and oxidative stress markers, urea, creatinine and serum creatinine levels in Patients with chronic renal failure. A total fifty CRF patients and fifty healthy control subjects were considered, based on the eGFR values patients were assigned to five phases. The results indicating that serum..........
Key words: Chronic renal failure (CRF), Oxidative stress, Serum Nitric oxide, Urea, Creatinine, Estimated glomerular filtration rate (eGFR)
[1]. Jha V, Garcia-Garcia G, Iseki K, Li Z, Naicker S, Plattner B, Chronic Kidney Disease: global dimension and perspectives, Lancet, 382(9888), 2013, 260-72.
[2]. Haslett C, Chilvers ER, Hunter A, Boon NA. edn, In: Davidson's Principle and Practice of Medicine, 18th edi. UK. 1999, 433-39.
[3]. Vaziri ND, Oxidative stress in uremia. Nature Mechanisms and Potential consequences, Seminars in nephrology, 2004, 469-73. [4]. Zalba G, Fortuno A, Diez J, Oxidative stress and atherosclerosis in early chronic disease, Nephrology Dialysis Transplantation, 21(10), 2006, 2686-2690. [5]. Luciak M, Antioxidants in the treatment of patients with renal failure, Annales Academiae Medicae Bialostocensis, 49, 2004, 157-161.
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| Paper Type | : | Research Paper |
| Title | : | Declines In Chill Coma Recovery and Negative Geotaxis Due To an Entamopathogen in Drosophila Melanogaster the Fruit Flies |
| Country | : | India |
| Authors | : | Usha Bhocal || Bijender Singh Sabharwal || Veer Bhan |
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: | 10.9790/264X-03032531 |
Abstract: Infection related studies in Drosophila melanogaster provide insight into both mechanisms of host resistance and pathogens tolerance.The advantages of the fly as an experimental system include its genetic tractability, short life span and the observable possibility and to quantitatively analyse behavioral responses. At present ,research has been limited by the relatively few metrics that can be used to measure health and illness during the course of infection. Here by we explain the pleiotropic measurements of infection-related declines in flies performance on two different assays. D..............
Key words: Chill coma, Drosophila, geotaxis, infection, resistance
[1] Sheldon, B. & Verhulst, S. (1996) Ecological immunology:costly parasite defences and trade-offs in evolutionary ecology. Trends in Ecology and Evolution 11, 317–321.
[2] Schmid-Hempel, P. & Ebert, D. (2003) On the evolutionary ecology of specific immune defence. Trends in Ecology and Evolution 18, 27–32.
[3] Medzhitov R, Schneider DS, Soares MP,(2012) Disease Tolerance as a Defense Strategy. Science335:936–941.
[4] Protzer U, Maini MK, Knolle PA (2012) Living in the liver: hepatic infections. 1–13.
[5] French SS, Denise Dearing M, Demas GE (2011) Leptin as a Physiological Mediator of Energetic Trade-Offs in Ecoimmunology: Implications for Disease. Integrative and Comparative Biology51:505–513.
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| Paper Type | : | Research Paper |
| Title | : | Screening and Optimization of L-Asparaginase Production From Bacillus Species |
| Country | : | India |
| Authors | : | Patil R.C. || Jadhav B. L. |
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: | 10.9790/264X-03033236 |
Abstract: L-asparaginase is an anti-neoplastic agent used in lymphoblastic leukaemia chemotherapy. In the present work a total of 5 soil samples were collected from various places near to Aurangabad. Soil microbial isolates were screened for potential producers of L-asparaginase using a phenol red indicator growth medium and the microbe producing the largest hydrolysis zone was selected. The isolate was characterized by biochemical tests and was found to belong to Bacillus sp. From these soil samples 12 isolates were identified as Bacillus licheniformis (5), Bacillus alvei (2), Bacillus megaterium (1), Bacillus cereus (1), Bacillus circulans (1) and Bacillus subtilis (2). The optimization study for the process parameters was given useful information about the production of the enzyme maximally. Potential L-asparginase producer isolates were showed the maximum activity at pH 7 and temperature at 37°C.
Key words: L-asparaginase, anti-neoplastic agent, Bacillus species
[1]. Sahu, M,K., Sivakumar, K., Poorani, E., Thangaradjou, T., & Kannan, L. (2007). Studies on L-asparaginase enzyme of actinomycetes isolated from esturine fishes, Journal of Environmental Biology, 28(2), 465-474.
[2]. Hill,J., Roberts,J., Loeb,E., Kahn,A. & Hill,R. (1967). L-asparaginase therapy for leukemia and other malignant neoplasm,JAMA, 202: 882.
[3]. Baskar,G., and Renganathan,S. (2011) Optimization of Media Components and Operating Conditions for Exogenous Production of Fungal L-asparaginase Chiang Mai J. Sci. 38(2) 270-279.
[4]. Roth,G., Volpato,G., Neves,C,E., Renard,G.,Basso,L,A., Chies,J,M., and Santos,D,S. (2010). Expression patterns of recombinant asparaginase in different E.coli strains. PUCRS.
[5]. Khamna, S.,Yokota, A., & Lumyong, S.(2009). L-asparginase production by actinomycetes isolated from some Thai medicinal plant rhizoshere soils. IJIB, 6-1, 22.
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| Paper Type | : | Research Paper |
| Title | : | Antidiabetic Activity of the Compounds Isolated From Rhus Mysorensis Plant Extract |
| Country | : | India |
| Authors | : | Ganji Renuka rani || Singara Charya MA || M. Viswanadham || Murali Krishna Thupurani |
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: | 10.9790/264X-03033742 |
Abstract: The current study was framed to evaluate the anti diabetic activity of falvonoids isolated from the leaves and rhizomes of Rhus mysorensis. Anti diabetic effects of these flavonoids were screened using in vitro and in vivo models. The in vitro anti diabetic activity was screened using enzyme assay. The in vivo anti diabetic activity was screened in albino diabetic induced rats. The inhibition of α-amylase and α-glucosidase by the isolated compounds were found in concentration dependent manner. The high inhibition was found at 150 mg/mL concentration. Among the compounds screened,....................
Key words: falvonoids, glibenclamide, Rhus mysorensis, α-amylase, α-glucosidase
[1] SD Sarker, Pharmacognosy in modern pharmacy curricula, Pharmacogn Mag, 8(30), 2012, 91–92.
[2] DK Patel, SK Prasad, R Kumar, S Hemalatha, An over view on anti-diabetic medicinal plants havening insulin mimetic property, Asian Pac J Trop Biomed, 25(4), 2012, 320-330.
[3] ANMM Rashid, Md Shamim Hossain, N Hassan, B Dash Kumar, Md. Ashrafuzzaman Sapon, M Kumer Sen, A review on medicinal plants with antidiabetic activity, Journal of Pharmacognosy and Phytochemistry, 3(4), 2014, 149-159.
[4] CL, Chang, Y Lin, AP Bartolome, YC Chen, SC Chiu, WC Yang, Herbal therapies for type 2 diabetic mellitus: Chemistry, Biology and Potential applications of selected plants and compounds, Evid Based Complement Alternat med, (2013), 378657,1-33.
[5] S Salim, Oxidative stress and psychological disorders, Curr. Neuropharmacol, 12 (2), 2014, 140–147.
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| Paper Type | : | Research Paper |
| Title | : | Current Understanding of Endophytes: Their Relevance, Importance, and Industrial Potentials |
| Country | : | India |
| Authors | : | Shashank A. Tidke || Rakesh Kumar KL || D.Ramakrishna || S.Kiran || G.Kosturkova || Ravishankar A. Gokare |
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: | 10.9790/264X-03034359 |
Abstract: Endophytes are microorganism which lives in endogenous association with host plants. They occur in plant of various habitat. This review focuses on the types of endophytes their classification and characterization and their beneficial effects on the plant productivity. Expression of various biochemical molecules in plants is a subject of intense research. Endophytes are known to produce metabolites of utility value for various applications. Biotechnological production of endophyte derived compounds both under in vitro & in vivo conditions have been investigated. The co-evolution of plant with endophytic association is beginnings to be understood. The current research on endophytes has been enumerated for comprehensive understanding of the topic.
Key words: Endophytes, classification, Secondary Metabolites, bioactive compounds.
[1] Leuchtmann, Systematics, distribution, and host specificity of grass endophytes J. Nat Toxins, 1, 1992 ,150-62.
[2] Hallmann, J., Quadt-Hallmann, A., Mahaffee, W.F., and Kloepper, J.W, Bacterial endophytes in agricultural crops. Can. J. Microbiol. 43, 1997,895-914.
[3] Freeman, E.M., Philos., Trans., R. Soc., Lond., [Biol]. 1904 ,196. 1.
[4] Huang, W.Y., Yi, Zhong, Cai., Kevin, D.H., Harold, C., and Mei, S, Endophytic fungi from Nerium oleander L (Apocynaceae): main Constituents and antioxidant activity J. World Journal of Microbiology and Biotechnology. 23,2007,1253-1263.
[5] Kusari, S., and Spiteller, M, Metabolomics of endophytic fungi producing associated plant secondary metabolites: progress, challenges and opportunities. In Metabolomics, U. Roessner, ed. (Rijeka, Croatia: InTech) 2012,241–266
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| Paper Type | : | Research Paper |
| Title | : | Isolation and Identification of Effective Hydrocarbon Degrading Bacteria from Diesel Polluted Soil |
| Country | : | India |
| Authors | : | Karthika R. || Archaya. S. || Nandhini . S. || Gopinath L. R. || Bhuvaneswari. R |
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: | 10.9790/264X-03036063 |
Abstract: Hydrocarbon pollution is due to accidental leakages, spills or by transportation. It might be carcinogenic to human, animal and plant health. Bioremediation is the best remedy to remove the pollutants from the environment. Diesel degrading microbes are widely distributed in our environment. The microbes may not be present in sufficient amount to achieve the degradation process in successfully. Inoculation of efficient hydrocarbon degrading bacterial strains in polluted area will enhance the capability of degradation. The present study was aimed to be isolating the effective bacterial strains from the polluted area to enhance the degradation. Totally fifteen isolates were identified and biochemically characterized.............
[1] Amund O.O. and Adebiyi A.G. 1991. Effect of viscosity on the biodegradability of automotive lubricating oils. Tribology International. 24: 235-237.
[2] Andrew, R.W.J. and J.M. Jackson, 1996 Pollution and waste management. In Environmental Science: The Natural Environment and Human Impact, Longman, Singapore Publishers Ltd., pp: 281-297
[3] Archaya, S., Gopinath, L.R., Sangeetha, S. and Bhuvaneshwari, R., 2014. Molecular characterization of kerosene degrading bacteria isolated from kerosene polluted soil. Int J Adv Res, 2(4), pp.1117-1124.
[4] Atlas, R. M.1992. Petroleum microbiology. In: Lederberg J (ed) Encyclopedia of microbiology. Academic press, Baltimore pp. 363-369.
[5] Bento, F.M., Camargo, F.A.O., Okeke, B. C., Frankenberger, I.F. 2005. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresource Technology. 96. 1049 – 1055.
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| Paper Type | : | Research Paper |
| Title | : | Calcium and Iron Rich Recipes of Finger millet |
| Country | : | India |
| Authors | : | Tahsin kazi || Sanjay Gajanan Auti |
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: | 10.9790/264X-03036468 |
Abstract: The Malnutrition is a serious matter of concern among the population of various countries, due to modern lifestyle specifically consumption of rapidly processed fast food. The present situation demands development of products that are rich in mineral nutrients, meet requirement of growth and development and readily acceptable. In this scenario, Finger millet is preferred as foremost choice for required minerals and low calorie diet. In the present work, 64 landraces were collected from Western ghat of Maharashtra. The collected landraces were analyzed for their mineral content...............
Key words: Finger millet, standardization of recipes, minerals analysis, organoleptic test.
[1] Chaturvedi, R. and S. Srivastava, 2008.Genotype variations in physical, nutritional and sensory quality of popped grains of amber and dark genotypes of finger millet.J. Food Sci. Technol., 45(5): 443-446.
[2] Wadikar, D.D., R.S. Premvalli, Y.S. Satyanarayanswamy and A.S. Bawa, 2007.Lipid profile in finger millet. J. Food Sci. Technol., 44(1): 79-81.
[3] Gopalan, C., B.V. Ramasastri and S.C. Balasubramanian, 2004.Nutritive value of Indian Foods. National Institute of Nutrition (NIN). Indian Council of Medical Research, Hyderabad, pp: 59-67.
[4] Sripriya, G., Usha, A., Chandra, T.S., 1997. Changes in carbohydrate, free amino acids, organic acids, phytate and HCl extractability of minerals during germination and fermentation of finger millet (Eleusine coracana). Food Chemistry 58(4), 345- 350
[5] Geetha C, Parvathi P (1990). Hypoglycemic effect of millet incorporated breakfast items on selected non-insulin dependent diabetic patients. Indian J. Nutr. Dietetics, 27: 316-320.
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| Paper Type | : | Research Paper |
| Title | : | Microalbuminuria as an Earliest Predictor of Diabetic Nephropathy |
| Country | : | India |
| Authors | : | Dr.SarikaD.Katore || Dr.Prasanna M. Fating |
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: | 10.9790/264X-03036972 |
Abstract: Diabetes mellitus is major health problem worldwidwe,characterised by persistent hyperglycaemia due to defect in insulin secretion, insulin action or both. Diabetic nephropathy is one of the major complications of diabetes mellitus.The present study aimed to asses urine microalbumin in patients of diabetes mellitus and diabetic nephropathy. This cross sectional study was undertaken in the Department of Biochemistry GMC and super speciality hospital Nagpur, Maharashtra.............
Key words: Diabetes mellitus, Diabetic nephropathy, Urinemicroalbumin, microalbuminuria
[1] Definition, Diagnosis and Classification of Diabetes Mellitus and its Complications WHO/NCD/NCS/99.2 Original: English Distr : General.
[2] King H, Aubert RE, Herman WH. Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care 1998; 21: 1414-1431.
[3] Update in Diabetic Nephropathy. Enyioma N Obineche and Abdu Adem.Int J Diabetes & Metabolism.2005; 13: 1-9 7.
[4] United States Renal Data System. USRDS 2007 Annual Data Report. Bethesda. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health U.S. Department of Health and Human Services, 2007
[5] Ramachandran A. Socio-economic burden of diabetes in India. J Assoc Physicians India. 2007 July; 55 : 9-12. 9.
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| Paper Type | : | Research Paper |
| Title | : | Effect of Cytokinins on Micropropagation of Anoectochilus elatus Lindl. from shoot tip explants – An Endangered Medicinal Orchid |
| Country | : | India |
| Authors | : | Dr. H. David Raja |
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: | 10.9790/264X-03037376 |
Abstract: Micropropagation of Orchids species has always been a challenging task. An efficient protocol for rapid Micropropagation of Anoectochilus elatus Lindley has been developed from shoot tip explants. Explants were cultured on Murashige and Skoog (MS) medium supplemented with BAP (6-Benzylamino purine 1 – 5 mg/l), TDZ (Thidiazuron 0.5 – 3.0 mg/l) and KIN (Kinetine 1 – 5 mg/l with 5% of Coconut Water (CW). 2.5 mg/l TDZ + 5% CW were proved to be best for high frequency multiple shoot induction from shoot-tip explants..............
Key words: Anoectochilus elatus, Ex vitro rooting, Micropropagation, Multiple shoots, Shoot tip.
[1] NV. Ket, EJ .Hahn, SY.Park, D. Chaharabarty, KY. Paek (2004). Micropropagation of an endangered orchid Anoectochilus formosanus. Biol. Plant. 48(3): 339-344.
[2] SC . Kumar, BV. Shetty, SSR. Bennet, T. Ananda Rao, S. Sanjay Molur (2001). Endemic Orchids of the Western Ghats. Conservation Assessment and Management Plan (CAMP) Workshop Report.
[3] C. Zheng , YZ. Huang, LF. Ji (1996). Pharmacognostical studies on Jin Xian Lian. Zhongcaoyao, 27: 169-172.
[4] T. Murashige and A. Skoog (1962). A revised medium for rapid growth and bioassays with Tobacco tissue culture. Physiol. Plant, 15 (1962) 473-497.
[5] [R D. Medina, EA. Flachsland, AM. Gonzalez, G. Terada, MM. Faloci and LA. Mroginski (2009). In vitro tuberization and plant regeneration from multi-nodal segment culture of Habenaria bractescens Lindl., an Argentinean wetland orchid. Plant Cell Tiss Organ Cult (2009) 97:91–101.