Version-1 (Sep–Oct 2016)
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| Paper Type | : | Research Paper |
| Title | : | IGF1 Gene As A Target For Genetic Diversity Among Indian Poultry Birds |
| Country | : | India |
| Authors | : | Ahmed Kareem Alatafi || kondapalli kasturi |
Abstract: The genes of Growth Hormone (GH) and Insulin-like Growth Factor 1 (IGF-1) are responsible for growth in animals as they play a key role in the regulation of their growth and development. The native breeds exhibit great Genetic polymorphism and there is a major concern for the necessity of preserving genetic resources. It is very important and significant to characterize and differentiate the genetically indigenous breeds.The present work deals with applying the RFLP.............
Key words: IGF1, RFLP, genetic diversity, chickens
[1] 1.Aminafshar Mehdi* and Fathi Ali Reza Single nucleotide Polymorphisms in intron 1 of growth hormone gene and it‟s association with economic important traits in Iranian Fars native fowl Scholars Research Library Annals of Biological Research, 2012, 3 (8):4028-4032 (http://scholarsresearchlibrary.com/archive.html)
[2] 2.http://agritech.tnau.ac.in/animal_husbandry/ani_chik_breeds%20of%20chicken.html
[3] 3. Tixier-Boichard, M., Bordas, A. & Rognon, X. 2008. Characterization and monitoring of poultry genetic resources. Proceedings of the 23rd World‟s Poultry Congress, Brisbane, Australia, July 2008. CD-ROM.
[4] 4. Robert Pym, Poultry genetics and breeding in developing countries Genetic diversity and conservation of genetic resources; Food and Agriculture Organization of the United Nations Poultry Development Review
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| Paper Type | : | Research Paper |
| Title | : | Computation of Melting Temperatures (Tm) of oligonucleotides for high throughput PCR using MS-Excel |
| Country | : | INDIA |
| Authors | : | Lavanya Kumari.P || Amaravathi.Y |
Abstract: DNA based molecular markers have enormous potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection. Most of the molecular markers are individually amplified by oligonucleotide primers using PCR. The efficiency of PCR depends on primer annealing temperature ( Ta) which optimize amplification and minimize non-specific binding of primers to the template. In contrast to the majority of the software programs available to calculate melting temperature (Tm) of primers/oligonucleotides which enables us to get Tm for single sequence/ input at a time..............
Key words: PCR, DNA based markers, Marker Assisted Selection (MAS,) Melting Temperature (Tm), Annealing Temperature (Ta), MS-Excel
[1] Breslauer, K.J., F. Ronald, H. Blocker, and L.A. Marky. 1986. Predicting DNA duplex stability from the base sequence. Proc. Natl. Acad. Sci. 83: 3746-3750.
[2] Freier, S.M., R. Kierzek, J.A. Jaeger, N. Sugimoto, M.H. Caruthers, T. Neilson, and D.H. Turner.1986. Improved free-energy parameters for predictions of RNA duplex stability. Proc. Natl.Acad. Sci. 83: 9373-9377.
[3] Howley,P.M., Israel,M.F., Law,M-F., and Martin,M.A. (1979) J BiolChem 254:4876-4883.
[4] Marmur,J., and Doty,P. (1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature, J Mol Biol 5:109-118.
[5] Nakano et al, (1999) Proc. Nuclec Acids Res. 27:2957-65.
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| Paper Type | : | Research Paper |
| Title | : | Is Lipid Accumulation Product (LAP), a better obesity index in diagnosing cardiovascular disease |
| Country | : | INDIA |
| Authors | : | Dr Nirmitha Dev M |
Abstract: Background: It has been shown that low grade inflammatory state is associated with cardiovascular risk. High sensitive C-reactive protein (hs-CRP), a nonspecific inflammatory marker has been shown to be increased in metabolic syndrome a risk state for the development of cardiovascular disease. Obesity is a predisposing condition to metabolic syndrome. Therefore, this study was intended to compare the indices available to measure obesity such as BMI and "lipid accumulation product" (LAP) with respect to risk for development of cardiovascular..............
Key words: Obesity, Lipid Accumulation Product (LAP), High sensitive C-reactive protein (hs-CRP), Body Mass Index (BMI).
[1]. World Health Organization Controlling the global obesity epidemic. Available at: http:// www. who. int/nutrition/ topics/ obesity/en/index.html
[2]. Trayhurn, P., Wood, I.S., (2004), "Adipokines: inflammation and the pleiotropic role of white adipose tissue", Br J Nutr 92:347–355
[3]. Weisberg, S.P., McCann, Desai, M., et al.(2003), "Obesity is associated with macrophage accumulation in adipose tissue", J. Clin. Invest, 112:1796-1808
[4]. Li, J-J., Fang, C.H., (2004), "C -reactive protein is not only an inflammatory marker but also a direct cause of Cardiovascular diseases", Medical Hypothesis, 62:499-506
[5]. Sartipy, P., and Loskutoff, D.J. (2003), "Monocyte chemoattractant protein-1 in Obesity and insulin resistance", Proc. Natl. Acad. Sci. U. S. A, 100:7265-7270.