Version-2 (Sep–Oct 2016)
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| Paper Type | : | Research Paper |
| Title | : | Antimicrobial Activity of Carica papaya, Piper nigrum and Datura stramonium Plants on Drug Resistant Pathogens Isolated from Clinical Specimens |
| Country | : | India |
| Authors | : | Dr. Nagesh Malik || Samreen Ahmed |
Abstract: The aim of the present study was to investigate the antimicrobial activity and phytochemical screening of Carica papaya, Piper nigrum and Datura stramonium plant extracts. Ethanol and Methanol were used as the solvents for extraction. The antimicrobial activities of these plant extracts were tested against the drug resistant Gram-positive and Gram-negative bacterial strains. The bacteria used in the study were Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter baumannii.............
Key words: Antibacterial activity, Drug resistant pathogens, ethanol, methanol, Phytochemical screening
[1]. Ajayi I. A., Ajibade O. and Oderinde R. A. 2011. Preliminary Phytochemical Analysis of some Plant Seeds. 1.
[2]. Behboud Jafari, Amirreza Ebadi, Babak Mohammadi Aghdam and Zarifeh Hassanzade, 2012. Antibacterial Activitiesof Lemon Grass Methanol Extract and Essence on Pathogenic Bacteria. American-Eurasian J. Agric. & Environ. Sci. 12:1042-1046.
[3]. Bipul Biswas, Kimberly Rogers, Fredrick McLaughlin, Dwayne Daniels and Anand Yadav. 2013. Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria. 2013:7.
[4]. C. Baskaran, V. Ratha bai, S.Velu and Kubendiran Kumaran. 2012. The efficacy of Carica papaya leaf extract on some bacterial and a fungal strain by well diffusion method. Asian Pacific Journal of Tropical Disease. 658-662.
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| Paper Type | : | Research Paper |
| Title | : | Subclinical Thyroid Dysfunction in Subjects with Metabolic Syndrome |
| Country | : | INDIA |
| Authors | : | Kartik Sekhar || Vanitha Gowda MN |
Abstract: Context: Metabolic Syndrome (MetSyn) is a clustering of interrelated cardiovascular risk factors. Subclinical hypothyroidism (SCH) is known to be a risk factor for adverse cardiovascular outcomes. The association between SCH and MetSyn has been found to be inconsistent. Aims: To determine the prevalence of SCH amongst subjects with MetSyn, and to study the association of serum TSH with components of MetSyn. Settings and Design: Thirty subjects with MetSyn and thirty healthy subjects were enrolled as cases and controls, respectively..............
Key words: Subclinical hypothyroid dysfunction, metabolic syndrome, Thyroid stimulating hormone
[1]. Pearce SHS, Brabant G, Duntas LH, et al. 2013 ETA Guideline: Management of Subclinical Hypothyroidism. European Thyroid Journal. 2013;2(4):215-228.
[2]. Jones DD May KE, Geraci SA. Subclinical thyroid disease. Am J Med. 2010 Jun; 123 (6):502-4
[3]. Marwaha RK, Tandon N, Ganie MA, Kanwar R et al. Status of Thyroid Function in Indian Adults: Two Decades After Universal Salt Iodization. JAPI. 2012 April; 60: 32-36
[4]. Grundy SM. Metabolic Syndrome: Connecting and Reconciling Cardiovascular and Diabetes Worlds. J Am Coll Cardiol. 2006;47:1093–100
[5]. Prasad DS, Kabir Z, Dash AK, Das BC. Prevalence and risk factors for metabolic syndrome in Asian Indians: A community study from urban Eastern India. J Cardiovasc Dis Res 2012;3:204-211.
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| Paper Type | : | Research Paper |
| Title | : | Raw grain characteristics and endoglucanase activity of sorghum (S. bi-color) and millet (Pennisetum typhoides and Digitaria exilis) malts |
| Country | : | Nigeria |
| Authors | : | D. I. Ukairo || H. C. Nzelibe || C. I. Iheme || C. P. Ihedimbu || A. U. Ezirim || S. Drisu |
Abstract: Endoglucanase activity has been a major concern in malting process due to the need to de-polymerize the high molecular weight cellulosic material. Sorghum and millet varieties were screened for their physicochemical properties and endoglucanase activity. Effect of plant hormone (gibberellic acid) on the production of endoglucanase during germination was also investigated. All the varieties screened apart from fonio-zegla, showed negligible water sensitivity, high germination energy and desirable germination capacity...............
Key words: Endoglucanase, germination energy, gibberellic acid, malting, millet, sorghum
[1] N. J. Parry, D. E Beever, E. Owen, W. Nerinckx, M. Clacyssens, J. Van Beeumen and M. K. Bhat. Biochemical characterization and mode of action of a thermostable endoglucanase purified from Thermoascus aurantiascus. Archives of Biochemistry and Biophysics, 404, 2002, 243 – 253.
[2] D. I. Ukairo, H. C. Nzelibe, C. I. Iheme, E. C. Udenze, C. P. Ihedimbu, and A. U. Ezirim. Purification and characterization of endoglucanase from sorghum (S. bi-color) and millet (Pennisetum typhoides and Digitaria exilis) malts. International Journal of Biochemistry Research, 8(4), 2015, 1-11.
[3] M. A. Milala, and E. O. Addy. Hydrolytic enzyme levels in malted cereals. Advances in Biochemistry, 2 (4), 2014, 76 -79.
[4] G. H. Palmer. (1989). Cereals in malting and brewing, in G.H. Palmer (Ed) Cereal science and Technology, (Scotland: Aberdeen University Press, 1989) 61-82.
[5] G. C. Gibbons, and E. B. Nielsen. (1983). New analyses in malting and brewing. Journal of Institue of Brewing, 89(1), 8 - 4.
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| Paper Type | : | Research Paper |
| Title | : | Chemistry, spectroscopic characteristics and biological activity of natural occurring cardiac glycosides |
| Country | : | Saudi Arabia |
| Authors | : | Marzough Aziz DagerAlbalawi |
Abstract: Cardiac glycosides are organic compounds containing two types namely Cardenolide and Bufadienolide. Cardiac glycosides are found in a diverse group of plants including Digitalis purpurea and Digitalis lanata (foxgloves), Nerium oleander (oleander),Thevetiaperuviana (yellow oleander), Convallariamajalis (lily of the valley), Urgineamaritima and Urgineaindica (squill), Strophanthusgratus (ouabain),Apocynumcannabinum (dogbane), and Cheiranthuscheiri (wallflower). In addition, the venom gland of cane toad (Bufomarinus) contains large quantities of a purported aphrodisiac substance that has resulted in cardiac glycoside poisoning...............
Key words: Heart failure, Cardenolides, Bufadienolide, Digitalis.
[1]. Fieser, L. and Fieser, M., Steroids, Reinhold Publishing Corporation, New York, Chapman & Hali, LTD., London 1959.
[2]. Evans, W.C., Pharmacognosy, 13th edition , Baillière Tindall, London, 1989.
[3]. Steyn, P.S. and van Heerden, F.R., Natural Products Reports 397, 1998.
[4]. Hesse, G. and Reicheneder, F., Ann. Chem 526, 1936.
[5]. Hesse, G.Heuser, L.J., Hütz, E. and Reicheneder, F., Ann. Chem. 566, 130, 1950.
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| Paper Type | : | Research Paper |
| Title | : | Study of Vitamin D levels in Mothers and Their New Borns |
| Country | : | India |
| Authors | : | Meera KS || Vanitha Gowda MN || Prarthana K || Pradeep G |
Abstract: Background: Adequate vitamin D concentrations during pregnancy are necessary to neonatal calcium homeostasis, bone maturation and mineralization. The aim of study is to evaluate vitamin D status in mothers and their newborns. Methods: 100 pregnant women were recruited from M.S.Ramaiah hospitals for the study and maternal blood was collected at the time of delivery. After the delivery of the new borns to the respective mothers, cord blood samples were collected. The samples were assayed for 25-hydroxyvitamin D3, calcium, phosphorus and alkaline phosphatase levels................
Key words: Calcium, cord blood, immunity, new borns, vitamin D.
[1]. Dawodu A, Wagner CL. Mother-child vitamin D deficiency: an international perspective. Archives of Disease in Childhood. 2007; 92(9): 737-740. [2]. Peechakara SV, Pittas AG. Vitamin D as a potential modifier of diabetes risk. Nature Clinical Practice Endocrinology & Metabolism. 2008; 4(4): 182-183.
[3]. Dixon KM, Norman AW, Sequeira VB, Mohan R, Rybchyn MS, Reeve VE, Halliday GM, Mason RS. 1α, 25 (OH) 2-Vitamins D and a Nongenomic Vitamin D Analogue Inhibit Ultraviolet Radiation–Induced Skin Carcinogenesis. Cancer Prevention Research. 2011; 4(9):1485-1494. [4]. Mirkovic K, van den Born J, Navis G, H de Borst M. Vitamin D in chronic kidney disease: new potential for intervention. Current drug targets. 2011; 12(1): 42-53. [5]. Youssef DA, Miller CW, El-Abbassi AM, Cutchins DC, Cutchins C, Grant WB, Peiris AN. Antimicrobial implications of vitamin D. Dermato-endocrinology. 2011; 3(4): 220-229..
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| Paper Type | : | Research Paper |
| Title | : | Subclinical Thyroid Dysfunction In Subjects With Metabolic Syndrome |
| Country | : | India |
| Authors | : | Kartik Sekhar || Vanitha Gowda MN |
Abstract: Context: Metabolic Syndrome (MetSyn) is a clustering of interrelated cardiovascular risk factors. Subclinical hypothyroidism (SCH) is known to be a risk factor for adverse cardiovascular outcomes. The association between SCH and MetSyn has been found to be inconsistent. Aims: To determine the prevalence of SCH amongst subjects with MetSyn, and to study the association of serum TSH with components of MetSyn. Settings and Design: Thirty subjects with MetSyn and thirty healthy subjects were enrolled as cases and controls, respectively................
Key words: Subclinical hypothyroid dysfunction, metabolic syndrome, Thyroid stimulating hormone.
[1]. Pearce SHS, Brabant G, Duntas LH, et al. 2013 ETA Guideline: Management of Subclinical Hypothyroidism. European Thyroid Journal. 2013;2(4):215-228.
[2]. Jones DD May KE, Geraci SA. Subclinical thyroid disease. Am J Med. 2010 Jun; 123 (6):502-4
[3]. Marwaha RK, Tandon N, Ganie MA, Kanwar R et al. Status of Thyroid Function in Indian Adults: Two Decades After Universal Salt Iodization. JAPI. 2012 April; 60: 32-36
[4]. Grundy SM. Metabolic Syndrome: Connecting and Reconciling Cardiovascular and Diabetes Worlds. J Am Coll Cardiol. 2006;47:1093–100
[5]. Prasad DS, Kabir Z, Dash AK, Das BC. Prevalence and risk factors for metabolic syndrome in Asian Indians: A community study from urban Eastern India. J Cardiovasc Dis Res 2012;3:204-211...
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| Paper Type | : | Research Paper |
| Title | : | Antioxidant Activity and Macropharge Cell Proliferation of KoreanBulgeun(Reddish) Mistletoe (ViscumAlbum For. RubroautiacumOhwi) |
| Country | : | Korea |
| Authors | : | Cheol Ho Park || Bo Duck Lee || In Je Sung || Timnoy Salitxay || Phonesavan Phouthaxay || Md Obyedul Kalam Azad || Byoung Jae Park |
Abstract: This study was conducted to investigate the food and medicinal functionality of Viscum album var. for. rubroaurantiaum hemiparasitic plant. Morphological characteristics, total polyphenol, total flavonoid, DPPH scavenging activity, macropharge cell proliferation, and nitric oxide production were analysedof four different plant parts such as leaf, node, bract, and fruit. Among the diferent plant parts investigated, content of total polyphenol ranged 21.1-24.6 mg/g TAE while total flavonoid ranged 5.5-10.7 mg/g RUE. The highest content of TP and TF were found in leaf, showing 24.6 mg/g TAE and 10.7 mg/g RUE, respectively.................
Key words: Antioxidant, cell proliferation, mistletoe, macropharge,nitric oxide,total phenol,Viscum album var. for. rubroaurantiaum
[1]. Deeni YY, and NM,Sadiq, Antimicrobial properties and phytochemical constituents of leaves of African mistletoe (Tapinanthus dodoneifolius (DC) Danser) (Loranthaceae) : an ethnomedicinal plant of Hausaland, Northern. J. Ethnopharmacol. 83,2002, 235-240.
[2]. D. Zuber, Biological flora of central Europe: Viscum album L. Flora. 199,2004,181-203.
[3]. CB.Lee, Korea flora, Hyangmunsa, Seoul, 1985,pp. 295-296.
[4]. SK.Kvangarnes, Phytochemical observation on European mistletoe. M.Sc. thesis,2009, pp.1-2, University of Bergen, Norway.
[5]. Alpsoy L, Uzonur I, Sakcali MS, and S, Karaman, Antioxidant and antimutagenic activities of Viscum album fruit ethanolic extract in human lymphocytes. African . J. Biotechnol. 9, 2010, 2539-2543.
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| Paper Type | : | Research Paper |
| Title | : | Isolation and Screening of Cellulolytic Fungi |
| Country | : | India |
| Authors | : | R. A. Kale || P. H. Zanwar |
Abstract: large number of microorganisms are capable of degrading cellulose, only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolyzing cellulose. Fungi are the main cellulase producing microorganisms. In this purposed study, four fungal species belonging to two genera i.e. Trichoderma and Aspergillus were isolated from different sources, screened and compared for their ability to degrade cellulose..................
Key words: Cellulase enzyme, Cellulolytic fungi, Trichoderma reesei, Aspergillus niger, Aspergillus fumigates, Aspergillus flavus, etc.
[1]. K. Chaudhary and P. Tauro, Sequential release of cellulase enzymes during germination of Trichodermareesei spores, Bioscience, Vol. 4, No. 3, 1982, 281-286.
[2]. T.W. Jeffries, M.S. Sykes, K. Rutledge-cropsey, J.H Klungness& S. Abubakar, Enhanced removal of tonners from office waste papers by microbial celluloses proceedings of the 6th International Conference on Biotechnology, 1996.
[3]. NaveenKumar K J and Thippeswamy B, Isolation and screening of potential cellulolytic fungi from Areca nut husk waste, INT J CURR SCI, 8: E, 2013, 125-132.
[4]. Ibatsam Khokhar, Muhammad Saleem Haider, Sobia Mushtaq, Irum Mukhtar, Isolation and Screening of Highly Cellulolytic Filamentous Fungi, Journal Applied Science Environmental Manage, September, Vol. 16 (3), 2012, 223-226.
[5]. P. Lakshmi Narasimha Reddy, B. Suresh Babu, A. Radhaiah and A. Sreeramulu, Screening, Identification and Isolation of Cellulolytic fungi from soils of Chittoor District, India, International Journal of Current Microbiology and Applied Sciences, Vol. 3 No. 7, 2014, 761-771.
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| Paper Type | : | Research Paper |
| Title | : | In Vitro Propagation of Date Palm (Phoenix dactylifera L.) Embryos Using Synthetic Seeds |
| Country | : | Egypt |
| Authors | : | Mohamed Ismail Diab || Reda El-Said Abo El-Fadl |
Abstract: Synthetic seed technology offers tremendous potential in micropropagation and germplasm conservation. Somatic embryos at the torpedo stage of Fraihy and Bartamuda date palm cultivars were used as synthetic seeds. They were encapsulated in sodium alginate matrix provided with Murashige and Skoog (MS) nutrient medium, antibiotic protectant (Cefotaxime) and without plant growth regulators (PGRs) as artificial endosperm to enhance germination and conversion percentage. The highest percentages of survival and germination were noticed with 3% sodium alginate, which gave 80.0% survival percentage for both cultivars, with germination percentage of 66.7 and75.5%.................
Key words: Date palm, somatic embryos, synthetic seeds, self- breaking gel beads
[1]. Abo Elfadl, R.E. (2008). Studies on in vitro propagation of Aswan date palm cultivars and their germplasm preservation. Ph.D. Thesis, Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City, Minufiya University, Egypt.
[2]. Ahmad, N. and M. Anis (2001). Direct plant regeneration from encapsulated nodal segments of Vitex negundo. Biol. Plant, 54: 748- 752.
[3]. Ali, A.; I. Gull; A. Majid; A. Saleem; S.Naz and N. H. Naveed (2012). In vitro conservation and production of vigorous and desiccate tolerant synthetic seeds in Stevia rebaudiana. J. Med. Plants Res. 6: 1327- 1333.
[4]. Al-Khayri, J.M. (2003). In vitro germination of somatic embryos in date palm: Effect of auxin concentration and strength of MS salts. Current Science, 5: 680- 683.
[5]. Alshuaibi, A. (2011).The econometrics of investment in date production in Saudi Arabia.Int. J. Applied Econ. Fainance, 5: 177-184.
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| Paper Type | : | Research Paper |
| Title | : | Direct colorimetric detection of canine distemper virus by RT-PCR combined with gold nanoparticles |
| Country | : | Taiwan |
| Authors | : | Y. L. Sun || H. T. Wu || C. S. Lin |
Abstract: Canine distemper is an acute systemic disease caused bycanine distemper virus (CDV), a RNA virus. CDV infection results in progress neurological signs of central nervous systemsystemically through the respiratory, digestive systems, and surrounding lymph tissues. It is therefore important to make an accurate rapid diagnosisin the early stages of the infection to prevent the disease from spreading. In this study, a rapid and simple gold nanoparticle (AuNP) colorimetric method was developed for CDV detection, named RT-PCR/AuNP assay. The assay is based on RT-PCR using specifically thiol-labeled primers to amplify the specific gene fragment of CDV. After mixing thiolated PCR amplicons with AuNP, and then the RT-PCR-AuNP products are formed by sulphur-gold linkage.................
Key words: Canine distemper virus; Colorimetric detection; Dog; Gold nanoparticle; RT-PCR
[1]. M.J.G.Appeal, and B.A.Summers, Pathogenicity of morbillivirus for terrestrial carnibores, Veterinary Microbiology, 44, 1995,187-191.
[2]. C.E.Greene, and M.Vandevelde, Canine distemper, inInfectious diseases of the dog and cat, Greene CE, (4thEd.), (St. Louis: Elsevier, 2012) 25-42
[3]. Von Messling, G.Zimmer, G.Herrler, L.Haas, and R.Cattaneo, The hemagglutinin of canine distemper virus determines tropism and cytopathogenicity, Journal of Virology, 75, 2001, 6418-6427
[4]. A. Cardoso, M.Blixenkrone-Moller, J.Fayolle, M.Liu, R.Buckland, and T.F.Wild, Immunization with plasmid DNA encoding for the measles virus hemagglutinin and nucleoprotein leads to humoral and cell-mediated immunity, Virology, 225, 1996, 293-299
[5]. N. Sixt, A. Cardoso, A. Vallier, J. Fayolle, R. Buckland, and T.F.Wild, Canine distemper virus DNA vaccination induces humoral and cellular immunity and protects against a lethal intracerebral challenge, Journal of Virology, 72, 1998, 8472-8476
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| Paper Type | : | Research Paper |
| Title | : | Rat Brain Glutathione S-transferasespotentially Defends Acrylamide Induced Neurotoxicity and Genotoxicity |
| Country | : | India |
| Authors | : | Sreenivasulu Dasari || Balaji Meriga |
Abstract: Acrylamide (AC) is a noxious chemical and it is widely used in this modernized world. There by this study aimedto assess the acrylamide induced neurotoxicity and genotoxicityonmale wistar rat brain. Based on the study aim, male at brainwas exposed by short term and long term to acrylamide and investigated the crucial changes in glutathione S-transferases and its associated glutathione peroxidase activities including degradation of DNA. Significant alterations of glutathione S-transferases and its associated glutathione peroxidase activities andinjured DNA results were documented. This study revealed that the glutathione S-transferases are potentially defends acrylamide toxicity to some extent but their activities aresignificantly decreasedby the accumulation of acrylamidein the brain and leads to degradation of DNA.................
Key words: Acrylamide, glutathione S-transferase, glutahtione peroxidase and genotoxicity
[1]. M. Friedman, Chemistry, biochemistry and safety of acrylamide A review, Journal of Agricultural and Food Chemistry, 51, 2003, 4504-4526.
[2] E. A. Smith, and F. W. Oehme, 1991. Acrylamide and polyacrylamide: a review of production, use, environmental fate and neurotoxicity, Reviews on Environmental Health, 9, 1991, 215–228.
[3] H. Deng, S. He, and S. Zhang, Quantitative measurments of vibration threshold in healthy adults and acrylamide workers, Intternational Archives of Occuppational and Environvironmental Health, 65, 1991, 53–56.
[4] T. O. Garland, and M. Patterson, Six cases of acrylamide poisoning, British Medical Journal, 4, 1967, 134–138.
[5] R. W. Tyl, M. C. Marr, C. B. Myers, W. P. Ross and M. A. Friedman, Relationship between acrylamide reproductive and neuro-toxicity in male rats, Reproductive Toxicololgy, 14, 2000, 147–157.
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| Paper Type | : | Research Paper |
| Title | : | Revealing ofWheat Products Contamination With Flour BeetlesTribolium spp. by Molecular Technique |
| Country | : | Egypt |
| Authors | : | Aida S.Kamel || Mona F. Abd-El Aziz || Rawheia H. Ramadan |
Abstract: Molecular technique is an accurate,susceptible, and species-specific method for detection of stored-product pests. The closely related flour beetles, Triboliumspp;red flour beetle, Triboliumcastaneum and confused flour beetle,Triboliumconfusum cause extensive economic loss of wheat grains and its products due to the contamination with their fragments and toxic secretions. PCR (Polymerase Chain Reaction) has been used successfully to detect DNA insect fragments in different commercial wheat product samples..................
Key words: Triboliumcastaneum,Triboliumconfusum, Primers, PCR, Wheat Flour, and Biscuits
[1]. Athanassiou, CG.; Kavallieratos, NG.;Palyvos, NE.;Sciarretta, A., Trematerra, P., (2005). Spatiotemporal distribution of insects and mites in Horizontally stored wheat. Journalof Economic Entomology, 98, 1058-1069.
[2]. Balasubramanian, A.;Jayas, DS.;Fernand, WGD.; Li G.; White, NDG.,(2007).Sensitivity analysis of DNA fingerprinting technique for detecting insect fragments in wheat flour Canadian Biosystems Engineering, 49: 4.1-4.5.
[3]. Beeman, RW.; Brown, SJ.,(1999). RAPD-based genetic linkage maps of Triboliumcastaneum. Genetics153: 333-338.
[4]. Bhuvaneswari, K.; Fields, PG.;White, NDG.;Sarkar, AK.;Singh, CB., (2011). Image analysis for detecting insect fragments in semolina. Journal of Stored Product Research 47(1): 20-24.
[5]. Blum, MS., (1981). Chemical defenses of arthropods. Academic Press, New York, pp. 183–205.
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| Paper Type | : | Research Paper |
| Title | : | Identification of pork (susscrofa), horse (Equuscaballus) and donkey (Equusasinus) by PCR-RFLP-DHPLC analysis on the mitochondrial COI gene |
| Country | : | China |
| Authors | : | Liping Song || Jie Jiang || Ting Mao || Na Shi || Wei Li || Jie Hao || Zhikai Hu || Miao Guo |
Abstract: Donkey flesh is a popular food in China. To ensure the quality of donkey products plays a vital role in protecting the interests of consumers. Pork and horse are potential in donkey flesh food because of their easier availability at cheaper prices. In present study, PCR-RFLP-DHPLC technology was established for distinguishing the pork, horseflesh and donkey flesh in donkey flesh food. The assay combined the species-specific primers to selectively amplify a short fragment of mitochondrial cytochrome c oxidase subunit I (COI) gene from a heterogeneous background of genomic DNAs followed by RFLP-DHPLC analysis...............
Key words: PCR-RFLP-DHPLC, donkey, COI gene
[1]. Wang, Q., et al., Identification of 12 animal species meat by T-RFLP on the 12S rRNA gene. Meat Sci, 2010. 85(2): p. 265-9.
[2]. Chisholm, J., et al., The detection of horse and donkey using real-time PCR. Meat Sci, 2005. 70(4): p. 727-32.
[3]. von Bargen, C., J. Brockmeyer, and H.U. Humpf, Meat authentication: a new HPLC-MS/MS based method for the fast and sensitive detection of horse and pork in highly processed food. J Agric Food Chem, 2014. 62(39): p. 9428-35.
[4]. Zhao, C.J., et al., Differentiating among horse (Equus caballus), donkey (Equus asinus) and their hybrids with combined analysis of nuclear and mitochondrial gene polymorphism. J Anim Breed Genet, 2005. 122(4): p. 285-8.
[5]. Soares, S., et al., Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay. Meat Sci, 2010. 85(3): p.
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| Paper Type | : | Research Paper |
| Title | : | Revealing ofWheat Products Contamination With Flour BeetlesTribolium spp. by Molecular Technique |
| Country | : | Egypt |
| Authors | : | Aida S.Kamel || Mona F. Abd-El Aziz || Rawheia H. Ramadan |
Abstract: Molecular technique is an accurate,susceptible, and species-specific method for detection of stored-product pests. The closely related flour beetles, Triboliumspp;red flour beetle, Triboliumcastaneum and confused flour beetle,Triboliumconfusum cause extensive economic loss of wheat grains and its products due to the contamination with their fragments and toxic secretions. PCR (Polymerase Chain Reaction) has been used successfully to detect DNA insect fragments in different commercial wheat product samples..................
Key words: Triboliumcastaneum,Triboliumconfusum, Primers, PCR, Wheat Flour, and Biscuits
[1]. Athanassiou, CG.; Kavallieratos, NG.;Palyvos, NE.;Sciarretta, A., Trematerra, P., (2005). Spatiotemporal distribution of insects and mites in Horizontally stored wheat. Journalof Economic Entomology, 98, 1058-1069.
[2]. Balasubramanian, A.;Jayas, DS.;Fernand, WGD.; Li G.; White, NDG.,(2007).Sensitivity analysis of DNA fingerprinting technique for detecting insect fragments in wheat flour Canadian Biosystems Engineering, 49: 4.1-4.5.
[3]. Beeman, RW.; Brown, SJ.,(1999). RAPD-based genetic linkage maps of Triboliumcastaneum. Genetics153: 333-338.
[4]. Bhuvaneswari, K.; Fields, PG.;White, NDG.;Sarkar, AK.;Singh, CB., (2011). Image analysis for detecting insect fragments in semolina. Journal of Stored Product Research 47(1): 20-24.
[5]. Blum, MS., (1981). Chemical defenses of arthropods. Academic Press, New York, pp. 183–205.