Abstract: Introduction: Carbapenems are considered to be the last choice for drug resistant Gram- negative bacteria but emergence of carbapenem resistant Gram-negative bacteria worldwide due to the production of carbapenemase has jeopardize their use. Aims and objectives: To detect the prevalence of carbapenem resistance in Gram-negative bacteria and carbapenemase production by Modified Hodge Test. Materials and Methods: A total of 160 isolates of Gram-negative bacteria from November 2012 to October 2014, from different clinical samples were included in the study. Those isolates with intermediate or resistant zone to meropenem and doripenem by disc diffusion methods according to CLSI guidelines were confirmed by E-test and were again tested for carbapenemase production by Modified Hodge Test (MHT).
[1]. Lee K, Lim YS, Yong D, Yum JH, Chong Y. Evaluation of the Hodge Test and the Imipenem-EDTA Double-Disk Synergy Test for Differentiating Metallo-β-Lactamase-Producing Isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol 2003;41(10):4623–629.
[2]. Priya D, Varsha G, Shivani G, Jagdish C. Phenotypic method for differentiation of carbapenemases in Enterobacteriaceae: Study from north India.Indian Journal of Pathology and Microbiology. 2012; 55 (3): 357-360.
[3]. Bradford PA, Urban C, Mariano N, Projan SJ, Rahal JJ, Bush K. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid mediated AmpC beta-lactamase and the foss of an outer membrane protein. Antimicrob Agents Chemother 1997;41:563- 9.
[4]. Andre B, Philippe B, Nathalie G, Catherine D, Dominique D, Guillaume A, Edouard B. Phenotypic Screening of Carbapenemases and Associated β-Lactamases in Carbapenem-Resistant Enterobacteriaceae. J Clin Microbiol. 2012 Apr; 50(4): 1295–1302.Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Twenty First Informational Supplement M100-S21. CLSI, Wayne, PA: USA; 2011. Available from: https://www.health.gov.au/internet/main/...nsf/.../cda-cdi3503c.htm . Accessed on Jan 18, 2014.