Version-6 (Nov-Dec 2016)
Version 1 Version 2 Version 3 Version 4 Version 5 Version 6
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Biosynthesis, Characterization and Study of Antimicrobial Activity of Copper and Silver Nanoparticles |
| Country | : | India |
| Authors | : | Heeral Pandya || Dr Nayna Chandak |
Abstract: Nanotechnology is gaining a lot of importance in various fields especially in medical field as many organisms are becoming resistant. Emerging infectious diseases and the increase in incidence of drug resistance among pathogenic bacteria have made the search for new antimicrobials inevitable. In the current situation, one of the most promising and novel therapeutic agents are the nanoparticles. Hence these nanoparticles can be used as antimicrobial agents to overcome this problem. In this study, copper and silver nanoparticles were made using a plant source and a microbial source. The synthesized nanoparticles were characterized using UV-Vis spectroscopy and FTIR. Also antimicrobial activity of these synthesized nanoparticles was checked for clinical isolates (fresh wound isolates and antibiotic resistant organisms) and MIC was carried out.
Keywords: Nanoparticles, antimicrobial activity, synthesis, silver, copper, characterization
[1]. Abboud Y, Saffaj T, Chagraoui A, Bouari A, Brouzi K, Tanane O, Ihssane B; Biosynthesis, characterization and antimicrobial activity of copper oxide nanoparticles (CONPs) produced using brown alga extract (Bifurcaria bifurcate); (2014); Applied Nanoscience; Vol 4; 571–576
[2]. Agarwal M, Murugan M, Sharma A, Rai R, Kamboj A, Sharma H, Roy S; Nanoparticles and its Toxic Effects; (2013); International journal of current microbiology and applied sciences; Vol 2(10); 76-82
[3]. Ahamed M, Alhadlaq H, Khan M, Karuppiah P, Al-Dhabi N; Synthesis, Characterization, and Antimicrobial Activity of Copper Oxide Nanoparticles; (2014); Journal of Nanomaterials; Vol 2, Article ID 637858; 1- 5
[4]. Andrews J; Determination of minimum inhibitory concentrations; (2001); Journal of antimicrobial chemotherapy; Vol 1; 5- 16
[5]. Angrasan J, Subbaiya R; Biosynthesis of Copper Nanoparticles by Vitis vinifera Leaf aqueous extract and its Antibacterial Activity; (2014); International journal of current microbiology and applied sciences; Vol 3(9); 768-774.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Prevalence of Nosocomial Infections within the Regional Hospital of Garoua |
| Country | : | Cameroon |
| Authors | : | GakeBouba, MD, Biologist || HimnoPahimi, Msc || GAKE Nelly-Michèle, MSc |
Abstract: Background: The health care-associated infections or nosocomial infections (NI) represent a universal public health problem. They are favored by the absence of regulations concerning their epidemiological monitoring, resulting in the increase of morbidity and mortality in hospitals. Thus, the main objective of our research was to determine the prevalence of NI within the Garoua Regional Hospital (GRH) following the European Center of Diseases Control (ECDC) criteria. Methods: Indeed, it was a cross-sectional study undertaken during the period extending from 22nd March to 22nd July 2014 using survey instruments and laboratory analyses..........
Keywords: Nosocomial infections, Prevalence, regional hospital, Garoua
[1] Becerra MR, Tantalean JA, Suarez VJ, Alvarado MC, Candela JL, Urcia FC. Epidemiologic surveillance of nosocomial infections in a Pediatric Intensive Care Unit of a developing country. BMC pediatrics2010; 10: 66.
[2] de Oliveira AC, Kovner CT, da Silva RS. Nosocomial infection in an intensive care unit in a Brazilian university hospital. Revista latino-americana de enfermagem 2010; 18(2): 233-239.
[3] Polin RA, Denson S, Brady MT. Epidemiology and diagnosis of health care-associated infections in the NICU. Pediatrics 2012; 129(4):1104-1109.
[4] Njall C, Adiogo D, Bita A, et al. [Bacterial ecology of nosocomial infection in intensive care unit of Laquintinie hospital Douala, Cameroon]. The Pan African medical journal 2013; 14: 140.
[5] Cardoso T, Almeida M, Friedman ND, et al. Classification of healthcare-associated infection: a systematic review 10 years after the first proposal. BMC medicine 2014; 12: 40
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Validated Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometric Method for Quantification of Rifaximin Human Plasma for Pharmacokinetic Study: ARandomized, Open-Label, Two-Period, Comparative Crossover Study in Healthy Korean Male Volunteers |
| Country | : | Korea |
| Authors | : | YongSun Park || Dong PilKim || Ki TakKim || Hong ChulPark || Yong Hwan Kim || Ye Na Kim || JinYoung Kim || Bo Ra Jin || Soo Ji Sim || Heon Soo Lee |
Abstract: A sensitive and specific liquid chromatography combined with electrospray ionization (ESI) tandem mass spectrometry (LC-MS/MS) method, operating in the positiveionization mode, for quantifying of rifaximin in human plasma using rifaximin-d6 as internal standard (IS) was developed and validated. The analyte and IS were extracted by simple one step liquid to liquid extraction (LLE). The chromatographic separation was performed on a Gemini C18 column (50 X 2.0 mm, 5 um) under isocratic conditions using a mixture of acetonitrile/10 mM ammonium formate (in 0.1% formic acid) (80:20, v/v) as mobile phase at a flow rate of 0.20 ml/min. Quantitation was performed using multiple reaction monitoring (MRM) mode to study parent...........
Keywords: Rifaximin, Human plasma, LC–MS/MS, Pharmacokinetics
[1] S. C. Choe, D. H. Lee. Use of Oral Antibiotics in Elderly Gastrointestinal Patients. J Korea Geriatr Soc. 16(3), 108-113, 2012
[2] Sama C., Morselli-Labate A. M., Pianta P., Lambertini L., Berardi S., Martini G. Clinical effects of rifaximin in patients with hepatic encephalopathy intolerant or nonresponsive to previous lactulose treatment: An open-label, pilot study. Curr Ther Res Clin Exp. 65(5), 413-422. 2004
[3] Venturini A. P. Pharmacokinetics of L/105, a new rifamycin, in rats and dogs, after oral administration. Chemotherapy. 29(1), 1-3, 1983
[4] Descombe J. J., Dubourg D., Picard M., Palazzini E. Pharmacokinetic study of rifaximin after oral administration in healthy volunteers. Int J ClinPharmacol Res. 14(2), 51-56. 1994
[5] Wang J., Wang J. L., Bao H., RP-HPLC determination of rifaximin. Chin. J. New Drugs, 12, 932-937, 2003
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Comparative Study of Synergic Effects of Spasmo Proxyvon with Some food Items |
| Country | : | India |
| Authors | : | Ksh.Mona Devi || A.K. Gupta || S.Joychandra || Munish Mishra |
Abstract: Spasmo proxyvon tablet is a popular antispasmodic drug and is covered under Narcotic Drugs. It is increasingly being abused for its opoid effect due to easily available. Recently, it has been being made cocktail with different food product to get stronger its effect. The present study was aimed to evaluate the type of food/beverage being use as cocktail with S.P.drug. In this study, tea, coffee, sweets and cigarette have been taken as enhancer of S.P drugs and Survey data were taken from 150 drug addicted volunteers.97 drugs abuser consumed tea,9 drug abuser use coffee. Sweets as cocktail using drug abusers were 6 in number.14 drug abusers used cigarettes as drug enhancer.............
Keywords: Spasmo Proxyvon, Dextropropoxyphene, Caffeine, Tea
[1] Venkata Raghava S,Vijay Kautilya D, Bheemappa Havanur, Devadass PK. Fatality From Spasmo-Proxyvon Addiction: A Few Cases. Journal of the Indian Society of Toxicology, Vol 6:47-49(2010)
[2] K Mnitombi Devi.An impact of drug abuse among youths in Manipur.International Journal in Commerce, IT & Social Science. Vol.2:62 (2005)
[3] Current awareness in drug testing and analysis. Published online in Wiley InterScience (2010)
[4] Gergov M, Nokua P, Vuori E, Ojanpera I.Univ Helsinki, Dept Forensic Med, POB 40, FI-00014 Helsinki, Finland Forensic Sci Int.Vol.186 :1-3 (2009) [5] Hans-Åke Lakso, Åke Norström.Determination of dextropropoxyphene and nordextropropoxyphene in urine by liquid chromatography–electrospray ionization mass spectrometry. Journal of Chromatography.Volume 794: 56-65 (2006)
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Biological Issues and Distribution of Mongolian Gerbil (Meriones Unguiculatus, Milne Edward, 1876) in Mongolia |
| Country | : | Mongolia |
| Authors | : | G. Munkhchuuun || N.Enkhbold || S.Amgalanbaatar |
Abstract: Grasslands are the dominant landscape in Mongolia, accounting for 70-80 % of the national land area.Spreading of Mongolian gerbil distribution throughout the Mongolia including sandy desert, sandy steppe, steppe and forest steppe, zones of pasture has been shown significantly harmful effects on wildlife habitat. The distribution, morphology characteristic of the rodent Mongolian Gerbil's survey undertaken by researchers Institute of Plant Protection Mongolian in 2012-2013 are described. The expedition trapped a sample of Mongolian gerbils (Scientific name) in different regions, eastern, western and central regions. During the survey expedition collected /261/ Mongolian gerbils.............
Keywords: Mongolian gerbils, rodents, distribution, morphology, behavior
[1] А.Г. Банников., Млекопитающие монгольской народной республики.,Москва 1954он.,407-415.,
[2] В.Е. Соколов., В.Н Орлов., Определитель млекопитающих Монгольской Народной Республики ., Москва 1980 он.,159-160.,
[3] Buyandelger S., Ts Munkhzul, R. P. Reading and J. D. Murdoch. 2009. Small ammal body measurements and density in the semi-desert of Ikh Nart Nature Reserve, Dornogobi, Mongolia. 2004-2009 report to the Denver Zoo Wildlife of the Mongolian Steppe program of Denver Zoo.
[4] Д. Цэвэгмид., Д. Цэнджав., Монгол орны хөхтөн амьтан., Улаанбаатар 1988 он
[5] Доржготов, Д. 2003. Монгол орны хөрс. ШУА-ийн Газарзүйн хүрээлэн. УБ.,
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Antipseudomonal Activities of Aqueous and Methanolic Peel Extracts of Carica papaya L. (Pawpaw) Unripe Fruits |
| Country | : | Nigeria |
| Authors | : | Sulayman Tunde Balogun || Assumpta Okoro Nwabugwu || Ayodele Oluwasoji Akanmu || Justus Jibrin || Samson Gamache |
Abstract: In response to the multi-drug resistance of Pseudomonas aeruginosa, the discovery of new effective agents is important in overcoming the infections resulting from the organisms. The present study assessed the antipseudomonal activities of aqueous and methanolic peel extracts of Carica papaya L. (pawpaw) unripe fruits against ten (10) multi-drug resistant (MDR) P. aeruginosa clinical isolates obtained from patients attending University of Maiduguri Teaching Hospital. The unripe fruit peels were extracted by maceration using distilled water and methanol (99%) and the antipseudomonal activities evaluated by modified diffusion method using concentrations of 20, 40, 80 and 160mg/ml...............
Keywords: Pseudomonas aeruginosa, Carica papaya L., Unripe fruit, Antibacterial activity
[1]. A.J. Afolayan, Extracts from the shoots of Arctotis artotoides inhibits the growth of bacteria and fungi. Pharmaceutical Biology, 41, 2003, 22-25.
[2]. J. Morton, Papaya: Carica papaya L., 2006, Retrieved on July 4th, 2015 from http://www.hort.purdue.edu/newcrop/morton/papaya_ars.html.
[3]. A. Sofowora, Medicinal plants and traditional medicine in Africa, 3 (Ibadan: Spectrum Book Limited, 2008).
[4]. K.D. Thomas and B. Ajani, Antisickling agent in extract of unripe pawpaw fruit (Carica papaya), Transaction of the Royal Society of Tropical Medicine and Hygiene, 81, 1987, 510-511.
[5]. G. Aravind, B. Debjit, S. Duraivel and G. Harish, Traditional and medicinal uses of Carica papaya. Journal of Medicinal Plants Studies, 1(1), 2013, 7-15.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Correlation of Serum and Urinary Magnesium with eGFR in Chronic Kidney Disease Patients |
| Country | : | India |
| Authors | : | SabithaThummala || Noorjahan.M || Priscilla Abraham Chandran |
Abstract: Aim And Objectives: The aim of our study is to correlate eGFR with serum and urinary magnesium levels in CKD patients. Material And Methods: This study is a prospective studywith total of 30 CKD patients(stages II-V) admitted in nephrology ward, males(n=21) and females(n=9), with mean age of 62.37±12.41 years. Serum urea, creatinine, magnesium and spot urine magnesiumwere estimated.eGFR was calculated by MDRD 4 variable equation. Pearson's correlation done.P value of <0.05 is taken as significant..............
[1] De Baaij JHF, Joost GJ et al. Regulation of magnesium bal-ance: lessons learned from human genetic disease. Clin Kid-ney J 2012; 5 (Suppl 1): i15–i24
[2] Graham L, Caesar J, Burgen A. Gastrointestinal absorption and excretion of Mg 28 in man. Metabolism 1960; 9: 646–659
[3] Navarro-Gonzalez JF, Mora-Fernandez C, Garcia-Perez J. Clinical implications of disordered magnesium homeostasis in chronic renal failure and dialysis. Semin Dial 2009; 22: 37–44
[4] Fine KD, Santa Ana CA, Porter JL, Fordtran JS. Intestinal ab-sorption of magnesium from food and supplements. J Clin Invest 1991; 88: 396–402
[5] Massry SG, Seelig MS. Hypomagnesemia and hypermagnese-mia. ClinNephrol 1977; 7: 147–153.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Entomotoxicity of Six Indegenous Plants Extracts in Controlling Callosobrochus Maculatus (Fabricius) (Coleoptera: Chrysomelidae) Infestation in Stored Vigna Unguiculata L.Walp. |
| Country | : | Nigeria |
| Authors | : | Mofunanya, A. A. J || Nta, A. I |
Abstract: Biological investigations were conducted to assess the insecticidal activities of n-hexane leaf extract of Solanum tuberosum, Annona muricata, Cymbopogon citrates, Vernonia amygddalina, Caesalpinia pulcherima and Lantana camara individually against the cowpea weevil Callosobruchus maculatus. The six plants leaf extracts showed significant (p≤0.05) insecticidal activities relative to control by increasing repellant and mortality rate, inhibiting oviposition and first filial generation progeny emergence and reducing seed damage................
Keywords: Callosobruchus maculatus, Entomotoxicity, Plant extracts, Vigna unguiculata
[1] M. Ndiaye, Ecology and management of charcoat rot (Macrophomina phaseolina) on the cowpea in the Sahel. Ph.D. thesis, Wagennigen University, The Nertherland with summary in English, French and Dutch, 2007, pp.114.
[2] C. O. Adedire, O.O. Obembe, R. O. Akinkurolele and O. Oduleye, Response of Callosobruchus maculatus (Coleoptera: Chysomelidae: Bruchidae) to extracts of cashew kernels. Journal of Plant Diseases and Protection. 118(2), 2011, 75-79.
[3] R. Bressani, Nutritive Value of cowpea. In S.R. Singh and K.O. Rachie (Eds.), cowpea research production and utilization. Wiley J., Sons Ltd., New York, USA, 1985, pp. 135-155.
[4] A. I. Nta, Y. B. Ibiang, E.A. Uyoh, N. E. Edu, B.E. Ekanem and Q.E. John, Insect pest damage to leaves of cowpea (Vigna unguiculata L. Walp): comparative effects of aqueous extracts of Piper guineensis, Allium sativum and Myristica fragrans. IOSR Journal of environmental Science, Toxicology and Food Technology. 3(2),2013, 17-20.
[5] M. K. Tripathy, P. Sahoo, B.C. Das and S. Mohanty, Efficacy of botanical oils, plant powders and extracts against Callosobruchus chunensis Linn. attacking blackgram (Cv. T9). Legume Research. 24, 2001, 82-86..
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | The Innate Differences in the Adipocyte Membrane Fatty Acid Composition Could Be Modified By Dietary Fats in Obese Mice |
| Country | : | Taiwan |
| Authors | : | Ming-Der Chen |
Abstract: Obesity-related insulin resistance is the risk factor for developing type 2 diabetes mellitus. The fatty acid composition of plasma membrane is known as a mediator to affect insulin action. This study aimed to investigate the differences in the adipocyte membrane fatty acid profile between genetically obese (ob/ob) mice and their lean (+/?) counterparts. In addition, the influence of dietary manipulation with different polyunsaturated/saturated (P/S) fatty acid ratio was also studied.................
Keywords: Adipose, Insulin resistance, Obesity, P/S ratio, Unsaturation index
[1]. M. M. Finucane, G. A. Stevens, M. J. Cowan, et al, National, regional, and global trends in body-mass index since 1980: systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9.1 million participants, Lancet, 377, 2011, 557-567.
[2]. P. E. Scherer, Adipose tissue: from lipid storage compartment to endocrine organ, Diabetes, 55, 2006, 1537-1545.
[3]. J. D. McGarry, Dysregulation of fatty acid metabolism in the etiology of type 2 diabetes, Diabetes, 51, 2002, 7-18.
[4]. C. Rattarasarn, Physiological and pathophysiological regulation of regional adipose tissue in the development of insulin resistance and type 2 diabetes, Acta Physiol, 186, 2006, 87-101.
[5]. M. T. Clandinin, S. Cheema, C. J. Field, M. L. Garg, J. Venkatraman, T. R. Clandinin, Dietary fat: exogenous determination of membrane structure and cell function, FASEB J, 5, 1991, 2761-2769.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Biomaterials of Marine Crustaceans – Prospects and Perspectives |
| Country | : | India |
| Authors | : | Ganga.G |
Abstract: Food processing produces large quantities of by-products. Disposal of waste can lead to environmental and human health problems, yet often they can be turned into high value, useful products. Crustacean shell wastes from shrimp, crab, lobster, and krill contain large amounts of chitin, a polysaccharide that may be extracted after deproteinisation and demineralization of the exoskeletons .crustacean shellfish wastes its derivatives, have many biological activities (e.g., anti-cancer, antioxidant, and immune-enhancing) and can be used in various applications................
Keywords: Biomaterial , Chitin ,Hemolymph,Marine, Industrial application, Pharmaceutical Application
[1]. W Sylvester Fredric and K.Ravichandran SAsian Pac J Trop Biomed. Jun; 2(6): 2012 496–502. doi: 10.1016/S2221-1691(12)60084-7 PMCID: PMC3609333
[2]. Glossary. Natural History Museum of Los Angeles County. Retrieved February 26, 2010.
[3]. "Species Fact Sheets: Scylla serrata". Food and Agriculture Organization. Retrieved September 2012.
[4]. A.D.Sowers,S.P. Young, , M. Grosell, C.L Browdy, andJ.R Tomasso,. "Hemolymph osmolality and cation concentrations in Litopenaeus vannamei during exposure to artificial sea salt or a mixed-ion solution: Relationship to potassium flux". Comparative Biochemistry and Physiology145: 2006. 176–180.
[5]. Y .Xu, M Bajaj, R Schneider,S. L Grage , A. S Ulrich, J Winter, andC.Gallert, Transformation of the matrix structure of shrimp shells during bacterial deproteination and demineralization. Microbial Cell Factories, 12, 2013,9089
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Identification and Enumeration of Bacterial Species From Rice Rhizosphere to Determine the Ratio of Nitrogen Fixers on Agricultural Aspect |
| Country | : | Bangladesh |
| Authors | : | Sazin Islam || Md. Masiuzzaman || Toriqul Islam || Muhammad Arif Iqbal || Md. Jahid Al Jakaria || Md. Mahabub Hasan || Md. Mohbub Alam |
Abstract: Soil sample was collected from agricultural land of Chuadanga district, Bangladesh for Microbial Characterization and Analysis of N2-Fixing bacterial species. The process was initiated with rice rhizosphere during seedling (after 30 days) of rice (BR 28, Oryza sativa L.). N-fertilizers mixing ration at that soil was 60kgha-1yr.-1. The soil was usually cultivated by Aus, Aman and Boro crops, as well as Rabi crops in winter. 1500 grams of crushed wet soil samples were collected. Drying process was initiated under 65°C for four days. 1227 grams samples were found after drying process...............
Keywords: Enterobacter spp., Klebsiella spp., Bacillus spp., Azospirllum spp.,Rhizosphere, Bangladesh
[1]. Collee, J.G. and Miles, R.S. (1989): Tests for identification of bacteria in Mackie and Maccartney practical medical microbiology.Churchill Livingstone, London. pp. 141-160.
[2]. Cottyn, B., Regalado, E., Lanoot, B., De Cleene, M., Mew T.W. and Swings, J. (2001): Bacterial Populations Associated with Rice Seed in the Tropical Environment. Phytopathology, 91, pp. 282-292.
[3]. Postgate, J. (1998). Nitrogen Fixation, 3rd Edition. Cambridge University Press, Cambridge UK
[4]. Postgate, J. R.(1982) The Fundamentals of Nitrogen Fixation. New York, NY: Cambridge University Press
[5]. Prescott, H. (2002) Laboratory Exercises inMicrobiology, 5thEdition, The McGraw−HillCompanies Riando, G. and Dommerques, Y. (1971): Validity of estimating biological nitrogen fixation in the
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Antimicrobial Activity of Cinnamonextracts against Foodborne Pathogens E.Coli, S.Tyhimurium and S.Aureus & L.Monocytogens |
| Country | : | India |
| Authors | : | Bharath M.R || Dr.M.A.Azeem || Sherien Basha || Keerthan H V |
Abstract: Solvents extracts of Cinnamon (Cinnamomum cassia) were analyzed for determination of antibacterial activity against four food borne pathogens: (4strains), two gram negative, Escherichia coli, Salmonella typhi, and two gram positive Staphylococcus aureus & listeria monocytogens .Screening of cinnamon extracts showed antibacterial activity against the test organisms. Cinnamon extracts showed antibacterial activity against all test bacteria with zone inhibition ranged from 10mm-35mm. MIC values for cinnamon is ranges from 23.04mg/kg to 64.44mg/kg.
Keywords: Antibacterial Activity, Cinnamon extracts, Food Borne Pathogens and Spoilage Bacteria.
[1]. Snyder, O. P., Antimicrobial effects of spices and herbs. Hospitality Institute of Technology and Management. St. Paul, Minnaesota. http ://www. hitm.com/Documents/Spices.html.(1997).
[2]. Shelef, L. A., Antimicrobial effects of spices. J.Food Safety, 6, 29-44(1983).
[3]. Hirasa, K., Takemasa, M. (eds.), Spices Science and Technology. Marcel Dekker Inc. New York, USA, pp1-232 (1998).
[4]. Nevas, M., Korhonen, A..R., Lindtrom, M., Turkki, P., Korkeala, H., Antibacterial efficiency ofFinnish spices essential oils against pathogenicand spoilage bacteria. J. Food Prot., 67, 199-202(2004).
[5]. Kim, J., Marshall, M. R., Wei, C., Antibacterial activity of some essential oil components against five food borne pathogens. J. Agric. Food Chem., 43, 2839-2845 (1994).
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Evaluating the Effect of PIH on Rats-Liver Overloaded with Iron |
| Country | : | Egypt |
| Authors | : | Marwan A. Ibrahim || Alaa-Eldin Salah-Eldin |
Abstract: The accumulation of iron (Fe) in the liver is a serious complication of thalassemia and other iron-loading anemias requiring long-term blood transfusions. Such patients are directed to suffer death unless they are treated with Fe chelating agents. Desferrioxamine (DFO), deferiprone (L1) and desferasirox (ICL-670) are clinically approved iron chelators used to treat secondary iron overload, but there is still need for new drug candidates due to limited long-term efficacy and drug toxicity. Pyridoxalisonicotinoylhydrazone (PIH) is tridentating iron chelator effective at scavenging and mobilizing iron. Therefore, the present study was undertaken to evaluate the role of PIH to improve rat antioxidant systems against iron overload (IOL) induced hepatic oxidative stress................
Keywords: Antioxidant, Iron overload, Lipid peroxidation, Liver, Oxidative stress, PIH.
[1]. Pulla Reddy A.C., Lokesh, B.R.Effect of curcumin and eugenol on iron induced hepatic toxicity in rats, Toxicology (1996) 9–45.
[2]. Bonkovsky, H.L. (1991):Iron and the liver. Am. J. Med. Sci., 301: 32: 43.
[3]. F.M.Ward, Daly Hepatic disease, in: R. Walker, C. Edwards (Eds.), Clinical Pharmacy and Therapeutics, Churchill Livingstone, New York, 1999, pp. 195–212.
[4]. D.A. Papanastasiou, D.V. Vayenas, A. Vassilopoulos, M. Repanti, Concentration of iron and distribution of iron and transferrin after experimental iron overload in rat tissues in vivo: study of the liver, the spleen, the central nervous system and other organs, Pathol. Res. Pract.196 (2000) 47–54.
[5]. L.S. Goodman, A. Gilman, The Pharmacological Basis of Therapeutics, 11th ed., McGraw-Hill, New York, 2006.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Co-Circulation Evidence of Dengue Virus Serotypes at the Kenyan Coast in 2014, 2015 |
| Country | : | Kenya |
| Authors | : | Katherine Munyuga || Joseph Ng'ang'a || Shingo Inoue || Charles K. Syengo || Caroline Ndege || A. Ole Kwallah || Kouichi Morita || Veronicah K. Anjichi || Matilu Mwau |
Abstract: Dengue virus is transmitted to humans by the Aedes aegypti and Aedes albopictus mosquitoes. These species of mosquito are ubiquitous in the Kenyan Coast. Dengue causes fever outbreaks and other fever associated symptoms that include Dengue fever (DF), Dengue Hemorrhagic fever's (DHF) and Dengue Shock Syndrome (DSS). Laboratory tests are almost always never done, and most patients are often given a course of antibiotics and painkillers after getting the diagnosis of fever of unknown origin. The study was done to determine the prevalence of DENV in the year of study and to provide evidence of co- circulation of DENV serotypes at the Kenyan Coast................
Keywords: ................
[1]. Henchal, E.A. and J.R. Putnak, The dengue viruses. Clin Microbiol Rev, 1990. 3(4): p. 376-96.
[2]. Gubler, D.J., Dengue and dengue hemorrhagic fever. Clin Microbiol Rev, 1998. 11(3): p. 480-96.
[3]. Amarasinghe, A., et al., Dengue virus infection in Africa. Emerg Infect Dis, 2011. 17(8): p. 1349-54.
[4]. Sang, R.C. and L.M. Dunster, The growing threat of arbovirus transmission and outbreaks in Kenya: a review. East Afr Med J, 2001. 78(12): p. 655-61.
[5]. ANNELIES WILDER-SMITH , S.Y., ARUL EARNEST, and R.S.A.N.I.I. PATON, Serological evidence for the co-circulation of multiple dengue virus serotypes in Singapore. Epidemiol. Infect. , 2005. 133( 667–671).
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Recent Analytical Trends in Lipidomics; Techniques and Applications in Clinical Medicine |
| Country | : | India |
| Authors | : | Manoj G Tyagi || Privy Varshney || Kapil D Sagar || Joyce Nirmala |
Abstract: Lipidomics is an emerging field of biomedical research which includes complex lipidome analysis. Basically it is the lipidome whichis the comprehensive and quantitative description of a set of lipid species present in an organism. Lipidomics encompasses the comprehensive analysis of molecular lipid species, including their quantitation and metabolic pathways. The wide diversity of native lipids and their modifications make lipidomic analyses challenging and difficult. The method of choice for sensitive detection and quantitation of molecular lipid species is mass spectrometry................
Keywords: Lipids, Lipidomics, analytical, chromatography, MALDI-TOF, spectrometry
[1]. M G Tyagi.Analysis of Phospholipids Using the High Performance Liquid Chromatography Technique.Int. J. Curr. Res. Biosci. Plant Biol. 2016, 3(4): 28-31
[2]. A Vijayakumar, P Vijayaraj, A Kumar Vijayakumar, and R Rajasekharan.The Arabidopsis ABHD11 Mutant Accumulates Polar Lipids in Leaves as a Consequence of Absent Acylhydrolase Activity.Plant Physiol. 2016 ; 170(1): 180–193.
[3]. Dennis, E.A, Deems, R.A, Harkewicz, R, Quehenberger, O, Brown, H.A., Milne, S.B., Myers, D.S, Glass, C.K, Hardiman, G., Reichart, D., Merrill Jr, A.H., Sullards, M.C., Wang, E., Murphy, R.C., Raetz, C.R, Garrett, T.A., Guan, Z, Ryan, A.C, Russell, D.W, McDonald, J.G, Thompson, B.M, Shaw, W.A, Sud, M, Zhao, Y, Gupta, S, Maurya, M.R, Fahy, E, Subramaniam, S.: A mouse macrophage lipidome. J. Biol. Chem. 2010,285, 39976–39985
[4]. T Mitchell. Lipidomics: Mass Spectrometry in a Big Fat World. Australia Biochemist. 46 No 3, 2015.13-23
[5]. Han, X.L.; Gross, R.W. Shotgun lipidomics: multidimensional MS analysis of cellular lipidomes. Expert Rev. Proteomics. 2005, 2, 253–264. 16.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Estimation of Crizotinib in Capsule Dosage Form by RP-HPLC |
| Country | : | India |
| Authors | : | Dr.Vijayakumar Bontha || Suman Emmadi || Sreekanth Gandhe |
Abstract: A simple, rapid, precise, sensitive and reproducible high performance liquid chromatographic method was developed for the estimation of Crizotinib in capsule dosage form. Chromatographic separation of the drug was achieved on a Syncronis C-18 (250 × 4.6 mm, 5μ) column, mobile phase comprising a mixture of methanol and buffer (60:40v/v) at a flow rate of 1 ml/min. The drug eluted was monitored at 267 nm, retention time, 4.15 min. & calibration curve was linear over the range of 10 μg-1000 μg/ml. The performance of the method was validated according to ICH guidelines. The method can be applied for determination of drug in its capsule dosage form without any interference from excipients. The proposed method is suitable for routine quality control analysis.
Keywords: Crizotinib, RP-HPLC, Validation.
[1]. Michael S. Roberts, David C. Turner, Alberto Broniscer, and Clinton F. Stewart. Determination of Crizotinib in Human and Mouse Plasma by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS). J Chromatogr B Analyt Technol Biomed Life Sci 2014; 960: 151–157.
[2]. Al-Majed, Nasr Y. Khalil, Tanveer A. Wani, Ibrahim A. Darwish, A. Highly Sensitive HPLC Method with Non-extractive Sample Preparation and Fluorescence Detection for Determination of Crizotinib in Human Plasma. Latin American Journal Of Pharmacy 2014; 33(6):1019-1026 ·
[3]. Wani T A, Iqbal M, Darwish I A, Khalil N Y, Zargar S. Development and validation of sensitive UPLC-Ms/Ms based method for the estimation of crizotinib in human plasma. Digest Journal of Nanomaterials and Biostructures2014;9(2): 693 – 704.
[4]. Feng Qiu, Yanan Gu, Tingting Wang, Ying ying Gao, Xiao Li, Xiang yu Gao and Shan Cheng. Quantification and pharmacokinetics of crizotinib in rats by liquid chromatography–tandem mass spectrometry. Biomedical Chromatography 2016;30(6): 962–968.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Impact of Disinfectants on Antimicrobial Potentials of Some Microorganisms |
| Country | : | Nigeria |
| Authors | : | Williams, Janet Olufunmilayo || White, Sukabari Precious |
Abstract: In hospitals and other health care sceneries, disinfectants are used expansively for a diversity of topical and hard-surface applications. They are vital parts of infection regulator exercises that also aid in the prevention of nosocomial infections. In order to avoid related diseases causedby pathogenic organisms, disinfectants are necessary for external use. This study investigated the effectiveness of varying commercial disinfectants for the control of pathogenic organisms. The clinical isolates used for the test were Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Epidermophyton floccosum while the commercial disinfectants used were liquid dettol.............
Keywords: Disinfectants, antimicrobial, effectiveness, experimental microorganisms.
[1] Awodele P.M., Emeka H.C, Agbamucheand Akintowa, A. (2007). The antimicrobial activities of some commonly used disinfectants on Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans. African j. of biotechnology, 6(8):987-990.
[2] Block, S.S., 1991. Disinfection, Sterilization and Preservation. 4th Edn., Lea and Febiger, Philadelphia.
[3] Cheesbrough M. (2002). District laboratory practice tropical countries part 2. Cambridge university press, UK.193-194.
[4] Denyer,S.P., Hugo W.B., Harding V.D. (2005). Synergy in preservative combination.Int. J. Pharm. 25: 245-253.
[5] El-Mahmood A.M, Doughari J.H. (2009). Bacteriological examination of some diluted disinfectants routinely used in the specialist hospital yola, Nigeria. African journal of pharmacy and pharmacology.3(5):185-190, htt://www.academicjournals.org/ajpp, s
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Bacteriological and Genetic Study of E-coli Isolated from different Infections in Diyala |
| Country | : | Iraq |
| Authors | : | Eman Abass Ali || Ibtihal Hameed Mohsin |
Abstract: The goal of present study was to Isolation and Identification of Escherichia coli from different Infections and detection the sensitivity and resistance of Ecoli to antimicrobials Additionally detection of virulence factors . This study was conducted from the period from 1 / 6 / 2016 to 1/ 9 / 2016 in Baquba city in Iraq.. It included; Fiftin samples were collected from different infections from Baquba General Hospital and AL-Batool Hospital. Twenty isolates were found to be Escherichia coli .The susceptibility test was applied on these isolates against deferent antibiotics. The results revealed that the highest resistances were for Piperacilline(93.3%) and highest Sensitive Imipenem with 100%, While the lowest resistance were for Tobramycin (9%)...........
Keywords: Escherichia coli , Extended Spectrumβ-Lactamases, Metallo β-Lactamases,Bacteriocin , Antibiotics
[1]. Hudault S, Guignot J, Servin AL (Jul 2001). "Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection". Gut. 49 (1): 47–55. doi:10.1136/gut.49.1.47. PMC 1728375. PMID 11413110 .
[2]. Nataro, J. P., Bopp, C. A., Fields, P. I., Kaper, J. B., & Strockbine, N. A. (2007). Escherichia, Shigella and Salmonella. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller (Eds.), Manual of Clinical Microbiology (9th ed., pp. 670-687). Washington, DC, USA: ASM press.
[3]. cookes1985, Feng, Peter, et al., "Enumeration of Escherichia coli and the Coliform Bacteria," in BACTERIOLOGICAL ANALYTICAL MANUAL (8th Ed. 2002), available online at http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods /Bacteriological Analytical Manual BAM/ucm064948.ht .
[4]. "Escherichia coli". CDC National Center for Emerging and Zoonotic Infectious Diseases. Retrieved 2012-10-02.
[5]. Wilson, W. R., Sande, M. A., & Drew, W. L. (2001). Current diagnosis & treatment in infectious diseases. New York: Lange Medical Books/McGraw-Hill.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Phytochemical, Antibacterial and Antioxidant Activity of Camellia Sinensis Methanolic and Aqueous Extracts |
| Country | : | Iraq |
| Authors | : | Noor S, Latteef |
Abstract: This study evaluated the antioxidant and antibacterial activity of green tea by using the methanolic and aqueous extract. phytochemical analysis also detected. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical. The methanolic crude extracts of green tea (Camellia sinensis) was the strongest, The EC50 of the methanolic extracts was 2.9 μg/ml and that of ascorbic acid was 2.9 μg/ml. Followed by BHT with value 4.0 μg/ml while The EC50 of the aqueous extracts was 6.5 μg/ml that showed less free radical scavenging activity with the DPPH method. The study reveals that the consumption of green tea has several beneficial effects by virtue of their antioxidant..........
Keywords: Antibacterial, Antioxidant, Camellia sinensis, DPPH, HPLC
[1]. Kukic, J,.Petrovic J and Niketic. antioxidant activity of tour endemic stachy a taxa. Bio, pharma. Bus. 29, 2006, 725-729.
[2]. Astley, S.B. Dictary antioxidants. Past, present and future. Trends. Food. Sci. Technol. 14, 2003, 93-98.
[3]. Farhoosh, R., Golmovahhed, G. A. and Khodaparast, M. H. H. Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chemistry. 100, 2007, 231 – 236.
[4]. Yilmaz, Y. Novel uses of catechins in foods. Trends in Food Science & Technology. 17, 2006, 64–71.
[5]. Si, W., Gong, J., Tsao, R., Kalab, M., Yang, R. and Yin, Y. Bioassay-guided purification and identification of antimicrobial components in Chinese green tea extract. Journal of Chromatography A. 1125, 2006, 204-210.