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| Paper Type | : | Research Paper |
| Title | : | Isolation and Identification of Agar Degrading Bacteria from Marine Environment |
| Country | : | India |
| Authors | : | Neeraja Kolhatkar || Dr. Seema Sambrani |
 |
: | 10.9790/3008-1303030107  |
Abstract: Agarolytic bacteria use agarase to utilize agar as sole source of carbon. It is usually observed in life sciences labs that lot of agar medium needs to be thrown after its usage. The media gets solidified and clogs the drainage systems. Hence there is a need to isolate agarolytic bacteria and use them to solve this pertinent problem for efficient and eco-friendly disposal of agar in labs. Further the enzyme that these organisms produce would also help to harness its several applications in food, cosmetic and medical industries as agarase produces oligosaccharides with remarkable activities after breaking down agar. In this study agarolytic bacteria were isolated from marine water source...............
Keywords: Agar, Agarase, Agarolytic Bacteria, Consortium, Enzyme.
[1]. Xiao Ting Fu and Sang Moo Kim (2010). Agarase review of major sources , categories, purification method, enzyme characteristics
and applications. Marine drugs 2010, 8, 200-218.
[2]. Won-Jae Chi and Yong-Keun Chang and Soon-Kwang Hong. Agar degradation by microorganisms and agar- degrading enzymes.
Appl Microbiol Biotechnol (2012) 94:917–930.
[3]. Linda J. Ross. The Enzymic hydrolysis of Neoagarotetraose, a thesis submitted to the Facu1ty of Graduate Studies and Research in
partial fulfi1lment of the requirements for the degree of Masters of Science Department of Microbio1ogy and Immuno1ogy McGi11
University Montreal June, 1970.
[4]. L.C.-M Chenl, J.S. Craigiel & Z.K. Xie (1993). Protoplast production from prophyra linearis using a simplified agarase producer
capable of commercial application. Journal of Applied Phycology 6: 35-39, 1994.
[5]. B. V. Hofsten and M. Malmqvist (1974). Degradation of Agar by a Gram-negative Bacterium. Journal of General Microbiology
Printed in Great Britain..
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| Paper Type | : | Research Paper |
| Title | : | Phytochemical and Antimicrobial Screening of Cocculus hirsutus L. |
| Country | : | India |
| Authors | : | Kumidini M || Ranganayakulu GS |
|
: | 10.9790/3008-1303030811 |
Abstract: Many number of the plant species including Cocculus hirsutus L. is being used as the sources of herbal medicine. Present work was mainly focused with the identification of the therapeutic properties of Cocculus hirsutus L. leaf extracts. The leaf extracts of methanol, aqueous, chloroform and benzene showed solvent dependent phytochemical present and anti-microbial activity. Whereas the leaf extracts of methanol and chloroform showed significant antimicrobial activity than water and benzene extracts. These extracts were further studied and analyzed for different phytochemical compounds. Leaf extracts possesses more flavonoids, steroids, alkaloids, tannins, glycosides and lignins in methanol, chloroform, benzene extracts than water extracts. The antimicrobial activity of different extracts of Cocculus hirsutus were analyzed and discussed...
Key words: Phytochemical screening, Anti-microbial activity, Cocculus hirsutus L.
[1]. De Pasquale A (1984) Pharmacognosy, The oldest modern science, Journal of ethanol pharmacology 11:1-16.
[2]. Adwan,G. & Mhanna, M. (2008). Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from
Clinical specimens. Middle-East Journal of Scientific Research 3 (3): 134-139.
[3]. Siddique KI, Uddin MMN, Islam MS, Parvin S and Shahriar M (2013) Phytochemical screenings, thrombolytic activity and
antimicrobial properties of the bark extracts of Averrhoa bilimbi. Journal of Applied Pharmaceutical Science 3(3): 94-96.
[4]. Parvin D, Uddin MMN, Islam MS, Parvin S, Shahriar M (2013) Phytochemical screenings, thrombolytic activity and antimicrobial
properties of the leaf extracts of Lablab parpureus. Am J Res Commun 1(2): 49-55.
[5]. Pushpangadan P, Rajasekharan R, Ratheshkumar PK, Jawahar CR, Velayudhan V, Nair, Lakshmi N, and Sarada Amma L( 1988)
Arogyappacha' (trichopus zeylanicus gaerin), the 'ginseng' of kani tribes of agashyar hills (kerala) for ever green healh and vitality.
Ancient Science of Life, Vol. VIII, Nos. 1. July 1988, Pages 13-16...
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| Paper Type | : | Research Paper |
| Title | : | Development and Validation of A RP-HPLC Method For Estimation of Flunitrazepam In A Tablet Dosage Form |
| Country | : | India |
| Authors | : | Debasis Bora || Prof(Dr) S K Gupta |
|
: | 10.9790/3008-1303031216 |
Abstract: A simple, sensitive, and precise high performance liquid chromatographic method for the analysis of Flunitrazepamhas been developed and validated for the determination of compound in commercial pharmaceutical products. The compounds were well separated on BDS Hypersil C18 reverse phase column by the use of a mobile phase of mixed phosphate buffer and acetonitrile in a ratio of 40:60 v/v, at a flow rate of 1.0 ml/min with detection wavelength at 248nm. The method was validated in terms of linearity, precision, accuracy, and specificity, robustness and solution stability. The method does require only 10 minutes as runtime for analysis which prove the adoptability of the method for the routine quality control analysis of the drug.
Key words:Flunitrazepam, RP-HPLC.
[1]. Gurudeepchatwal, G.R. Anand, Instrumental methods of chemical analysis, ed-5th, Himalaya Publishing house, New Delhi., PP.2.624-2.629, 2007.
[2]. Skoog D.A.Holler, F.G.Nieman., Principles of Instrumental Analysis, ed-5th, Thomson Brooks/Cole Asia Pvt.Ltd., Singapore, PP.4-7, 2004.
[3]. Hobort, H.Willard, Instrumental methods analysis, ed-2nd, JP publishers, New Delhi, PP.529-563, 1986.
[4]. Sethi P.D., High performance liquid chromatography,ed-1st,CBS publishers and distributors, New Delhi,PP.12-15,2001
[5]. Anjaneyulu.Y & Marayah, Quality assurance & Quality management in pharmaceutical industry, ed-4th, pharma book publishers, Hyd, PP.78-108, 2005..
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| Paper Type | : | Research Paper |
| Title | : | How To Over Come Antibiotic Drug Resistance? Drug Resistance is Disorder of Second Order Of Universe Either By Drug Itself Or By Alteration In Implementation Due To Changed Behavior (Mutation) Of Target B.B.Bs. This Second Order of Universe Could be Reset by First Order Of Universe (AGE). |
| Country | : | India |
| Authors | : | Dr V M Das |
|
: | 10.9790/3008-1303031746 |
Abstract: Any disorder in implementation of second order of universe carried by drug by alteration in behavior of target B.B.Bs ( mutations ) , drug resistance develop . Drugs or antibiotics are set of information's (Code PCPs bactericidal / bacteriostatic activity ) that trigger ( second order carried by photons ) bactericidal / bacteriostatic effects by giving specific order to bacterial cell . If this order ( Code PcPs bactericidal / bacteriostatic activity ) is failed to deliver ( drug half life or dose related ) to bacterial cell or failed to implement due to changed behavior by target B.B.Bs ( due to mutation ) , drug resistance would be there . This disorder could be reset by certain drugs when added with antibiotics . Resistance-modifying agents are capable of partly or completely suppressing bacterial resistance...............
Keywords: Basic Building Blocks , Mind and Mass , Atomic genetics , Atomic transcription and Translation , Tachyons
[1]. V.M.Das IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) Volume 12, Issue 5 Ver. VI (Sep. – Oct. 2017), PP
08-42 https://en.wikipedia.org/wiki/Antibiotics
[2]. V M Das International journal of innovative research and development Volume 3 Issue 8 p 194- 210 (2014)
[3]. V M Das International Journal of Sciences: Basic and Applied Research (IJSBAR) Volume 17, No 1, p 292-324 (2014)
[4]. V M Das International Journal of Sciences: Basic and Applied Research,Volume 17, No 1, p 148-185 (2014)
[5]. V M Das International Journal of Sciences: Basic and Applied Research) Volume 16, No 2, p 274-309 (2014).
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| Paper Type | : | Research Paper |
| Title | : | The Effect of the Chromatographic Fractions of Bares Precatorius Leaf on the Reproductive Hormones Using Female Westar Rats |
| Country | : | Nigeria |
| Authors | : | N.D. Ajibo || I.H.Ogbuehi || A.W Obianime || N. Brambaifa |
|
: | 10.9790/3008-1303034756 |
Abstract: AIM: To determine the effect of chromatographic fractions of Abrus precatorius extract on female reproductive Hormones in Wistar Rats. STUDY DESIGN: Female wistar rats were treated with chromatographic fractions of A. precatorius, F1, F2,F3 and F4 (30mg/kg, 60mg/kg,90mg/kg, 120mg/kg and 150mg/kg) for 4 estrus cycles. The hormones studied are Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Progesterone (P4), Estrogen (E2) and Prolactin (PRL). On the day 16 (4 estrous cycle) the animals were sacrificed, dissected and the uterus and ovaries obtained for histology. Some Liver enzymes such as Alanine Transferase (ALT) , Alanine Phosphate (ALP) and Aspartame Transferase (AST) were also studied to evaluate the effect of Abrus precatorius fractions on liver functions.
[1]. Adetoro, O.O, Ebomoyi, E.W (1997) "The Prevalence of Infertility in a rural Nigerian community. African Journal of Medicine and Medical Sciences‟‟. 20:23-27 (Pubmed).
[2]. Akhila, J.S., Deepa,S and Alwar, M.C (2007). "Acute toxicity studies and determination of median lethal dose". Current Science. 93(7) 917-920 Albino Mice.
[3]. Allan, A.L.,William, J.W., Bettina, H (2017) "Centre for Occupational and Environmental Medicine‟‟.vol 3.
[4]. Alvaro Viera, R. Subsidio Para O Estudo (1959). "Da Flora Medicinal Da Guinea Portuguesa Agencia-Geral Do Ultramar Lisboa‟‟. Am. J. Pharmacol. Toxicol. 10(2): 40.45
[5]. Anbu, J., Ravichandiran, V., Sumithra M., Chowdary, S.B., Swaroop Kumar,S. Kannadhasan, R. and Kumar, R.S (2011). "Anticancer activity of petroleum ether extract of
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| Paper Type | : | Research Paper |
| Title | : | Qualitative and Quantitative Analysis of Cissus Quadrangularis Using Different Extracts |
| Country | : | India |
| Authors | : | Sadhana. B. K || Gopinath, L. R || Archaya. S || Rajamuni. P || Suresh Kumar, B. T |
|
: | 10.9790/3008-1303035760 |
Abstract: C. quadrangularis Linn is very important medicinal plant which has potassium, calcium, zinc, sodium, iron, lead, cadmium, copper, calcium oxalate and magnesium. In this study qualitative and quantitative analysis of extracts of C. quadrangularis with different solvents was analysed and the presence of the phytochemicals were noticed. The quantity of Alkaloids, Flavonoids, Terpenoids, Glycosides and Saponins were found to be higher in methanol extract, tannins and steroids was high in aqueous extract..
Keywords: Basic Building Blocks , Mind and Mass , Atomic genetics , Atomic transcription and Translation , Tachyons
[1]. Barker, D. J. P. 1996. Growth in utero and coronary heart disease. Nutrition Reviews 54, SI-S7.
[2]. Blytt, H. J., Guscar, T. K., Butler, L. G., 1988. Antinutritional effects and ecological significance of dietary condensed tannins may not be due to binding and inhibiting digestive enzymes. J Chem Ecol. 14, 1455-1465.
[3]. De Bruyne, T., Pieters, L., Deelstra, H., Vlietinck, A. 1999. Condensed vegetables tannins: biodiversity in structure and biological activities. Biochem Syst Ecol 27:445–59.
[4]. Dolara, P., Luceri, C., De Filippo, C., Femia, A. P., Giovannelli, L., Carderni, G., Cecchini, C., Silvi, S., Orpianesi, C., Cresci, A. 2005. Red wine polyphenols influence carcinogenesis, intestinal microflora, oxidative damage and gene expression profiles of colonic mucosa in F344 rats. Mutation Research, 591:237–46.
[5]. Farooq, A., Sajid, L., Muhammad, A., Anwarul-Hassan, G. 2007. Moringa oleifera: a food plant with multiple medicinal uses. Phytotheraphy Research. 21:17-25..
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| Paper Type | : | Research Paper |
| Title | : | Novel Method of Formulation, Optimization and Inviter Evaluation of Eudragit Fs30d Coated Tablets with Biodegradable Polymers for Targeted Colonic Delivery of Azathioprine by Core-In-Cup Tablet Dosage Form |
| Country | : | India |
| Authors | : | K. Malleswari || J. Vijaya Ratna |
|
: | 10.9790/3008-1303036173 |
Abstract: Colonic delivery has gained increasing importance for the delivery of drugs for the treatment of local diseases. To achieve a successful colonic delivery a drug needs to be protected from absorption in the GI tract and abruptly release in to the proximal colon which is considered the optimum site for colon-targeted delivery of drugs. The purpose of this study is to explore the possibility of core in cup tablet as a novel carrier for colonic delivery of a sparingly soluble drug, Azathioprine.The study involves designing a tablet for colon-target which consists of a central blended form of drug and polymer core and were further coated with different concentrations of Eudragit FS30D to thin film and fine thickness and finally the core-in-cup is over coated with pH-sensitive polymer, by spray coat........
Keywords: Azathioprine, Biodegradable gums, Eudragit, film coat, core in cup tablet
[1]. Ashford M, Fell JT. Targeting drugs to the colon: delivery systems for oral administration. J Drug Target. 1994; 2:241–57.
[2]. Rubinstein A. Approaches and opportunities in colon-specific drug delivery. Crit Rev Ther Drug Carrier Syst. 1995;12:101–49.
[3]. Watts PJ, Lllum L. Colonic Drug Delivery. Drug Dev Ind Pharm. 1997;23:893–913.
[4]. Kinget R, Kalala W, Vervoort L, Van Der Mooter G. Colonic drug targeting. J Drug Target. 1998;6:129–49.
[5]. Niwa K, Takaya T, Morimoto T, Takada K. Preparation and evaluation of a time-controlled release capsule made of ethylcellulose
for colon delivery of drugs. J Drug Target. 1995;3:83–9...
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| Paper Type | : | Research Paper |
| Title | : | Utilization of Blood Transfusion Services Provided by Al-Baha Central Blood Bank |
| Country | : | Saudi Arabia |
| Authors | : | Abuobaida E. Abukhelaif || Ahmed G. AlgHamedi || Abdullah M.AlFalah |
|
: | 10.9790/3008-1303037477 |
Abstract: Introduction: Blood transfusion plays vital roles in the medical and surgical practice. Blood safety is one of the most important issues in blood transfusion. Blood and blood componentsare frequently ordered and utilized without proper analysis of the real needs.Objectives: The main objectives of this study were toevaluate blood transfusion utilization at Al-Baha Central Blood Bank during the period 2012 -2015Methods: a retrospective 4-year analysis from January 2012 to December 2015 at Al-Baha Central Blood BankResults: In this study, the most common donors were Saudi donors (89.2%), volunteer blood donors comprised. The highest percentage (90.3%) of collected blood and 85.4% were crossed matched and issued to different hospital in the region.blood group O+ was the mostcommonly utilized type of blood........
Keywords: Blood transfusion services, voluntary blood donation,blood utilization, Al-Baha Central Blood Bank
[1]. Dutta AB. Transfusion practice clinical aspects and applications. In: Blood Banking and Transfusion 1st Ed. New Delhi: CBS Publishers; 2006.
[2]. Chawla T, Kakepoto GN, Khan MA. An Audit of Blood Cross-match ordering practices at the Aga Khan University Hospital: First step towards a Maximum Surgical Blood ordering Schedule . J Pak Med Assoc 2001; 51 : 251-54
[3]. Ankra-Badu GA, Ahmad MS, Sowayan SA, Bashawri L. Demographic characteristics of seropositive donors in Al-Khobar. Annals of Saudi Medicine 2001; 21: 113-116.
[4]. WHO-EM/LAB/327/E/L Report on the REGIONAL MEETING OF DIRECTORS OF BLOODTRANSFUSION SERVICES, Teheran, Islamic Republic of Iran, 16-20 October 1999)
[5]. Safe blood and blood products: indicators and quality of care. World Health Organization, Regional office for the western Pacific,Manila, Philippines. October 1996..
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| Paper Type | : | Research Paper |
| Title | : | Interactions ofMoringaoleifera Seed (Lam) With Cations Transport Adenosine Triphosphatasesin Protein Energy Malnourished Rats Skeletal Muscle |
| Country | : | Nigeria |
| Authors | : | Jegede, Eneji Raphael || Bewaji Clement Olatubosun || Didunyemi |
|
: | 10.9790/3008-1303037885 |
Abstract: The aim of the present study was to evaluate the interactions of dietary supplementation ofdefatted Moringaoleiferaseed (lam) with cations transport adenosine triphosphatases in skeletal muscle of muscle degenerated rats induced by Protein Energy Malnutrition (PEM). PEM has been associated with skeletal muscle degeneration and calcium homeostasis dysfunction. In the course of the experiment, twenty four (24) weanling female albino rats (Rattusnorvegicus) of the Wistar strain (average weight of 49.39±1.73) were used for this investigation. (PEM) was induced in eighteen (18) rats by feeding them with low protein diet and water for twenty eight days while the remaining six rats served as Positive control groupand received commercial feed containing 21% crude protein. These PEM........
[1]. Adelusi, S. A, and Olowokere, J.O (1985): A Rapid method of induction of postnatal protein-energy malnutrition (PEM) in laboratory animals. Niger. J. Appl. Sci 3: 171-174.
[2]. Anwar, F. andBhanger, M., I. (2003): Analytical characterization of Moringaoleiferaseed oils grown in temperate regions of Pakistan. J. Agric. Food Chem. 51: 6558-6563.
[3]. Anwar, F. Latif, S. Ashraf, M. and Gilani, A. H. (2007):Moringaoleifera, a food plant with multiple medicinal uses, Phytotherapy Research, vol. 21, No. 1, Pp 17–25.
[4]. Bababunmi E.A. (2002): Formulation and Method for Treating Skeletal Muscle Degeneration Caused By Malnutrition and Disease.United States Patent 6887499
[5]. Barzel, U. S. and Massey, L. K. (1998): Excess dietary protein can adversely affect bone. The Journal of Nutrition Vol. 128 (6) 1051-1053...
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| Paper Type | : | Research Paper |
| Title | : | Microarray Data: A Powerful Method for Identifying Differentially Expressed Genes |
| Country | : | |
| Authors | : | Md. Bipul Hossen || Md. Javed Ali || Mst. Noorunnahar |
|
: | 10.9790/3008-1303038694 |
Abstract: Microarray technology observing thousands of gene expressions at once, has been the most popular research topics in recent decades. The new data promise to improve fundamental understanding of life on a molecular level and may prove very helpful in medical diagnosis, treatment and drug design. Identification of differentially expressed (DE) genes across tissue samples or experimental conditions for the analysis of microarray data is the greatest challenge nowadays. Several approaches have already been used to improve the identification of DE genes. In this study, the most popular methods such as Significance Analysis of Microarrays, two Samples Mean Test........
Keywords: Microarray Technology, Identification of Differentially Expressed Genes, False Discovery Rate
[1]. Baldi, P., Long, A.D.; A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes. Bioinformatics 2001; 17: 509-519.
[2]. Smyth, G.K.; Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Statistical Applications in Genetics and Molecular Biology 2004; 3: Article 3.
[3]. Bokka, S. and Mathur, S.; A nonparametric likelihood ratio test to identify differentially expressed genes from microarray data. Appl Bioinform 2006; 5(4): 267–276.
[4]. Troyanskaya, O.G., Garber, M.E., Brown, P.O., Botstein, D and Altman, R.B.; Nonparametric methods for identifying differentially expressed genes in microarray data. Bioinformatics 2002; 18(11): 1454–1461.
[5]. Qin, H., Feng, T., Harding, S.A., Tsai, C-J and Zhang, S.; An efficient method to identify differentially expressed genes in microarray experiments. Bioinformatics 2008; 24(14): 1583–1589..